EC11 anti-Pericardin

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$40.00
SKU: EC11 anti-Pericardin
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DSHB Data Sheet

Catalog Fields

Antigen: Pericardin
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Gratecos, Danielle
Isotype: MIgG3
Depositors Institution: Laboratoire de Genetique et Physiologie du Developpement (LGPD) / CNRS
Host Species: mouse
Depositors Notes: Fusion: 1986. Immunohistology: On whole mount Drosophila embryos, it decorates the extracellular matrix surrounding the pericardial cells and the cardial cells of the heart tube, from 10h embryogenesis onwards; it is synthesized and secreted by the pericardial cells; it also labels the ring gland and the oenocytes; no maternal expression; it is still present around the adult heart tube. Immunoblotting with EC11: three bands (200, 190, 165 kDa) differentially expressed during embryogenesis, the 165 kDa species being the only one to persist in the adult heart tube. Functional effects: In the absence of Pericardin, the heart epithelium appears disorganized during its migration in concert with the dorsal ectoderm. The alignment of the cardial and pericardial cells is interrupted by holes and both types of cells are found accumulated in clusters along the rows of cardioblasts. The protein is probably involved in the morphogenesis and in the maintenance of the heart epithelium during dorsal ectoderm closure. Immunohistochemistry: Pericardial cells, extracellular matrix surrounding the heart tube, ring gland, oenocytes.
Positive Tested Species Reactivity: Drosophila
Gene: prc
Antigen Molecular Weight: Predicted: 165kDa; Apparent: 200, 190 and 165kDa
Alternate Gene Names: pericardin; pc
Immunogen: Nitrocellulose containing embryonic (10-14 hours Drosophila melanogaster, Oregon R embryos) proteins after western blot. The excised nitrocellulose band contained proteins migrating at the same level than myosin heavy chain.
Clonality: Monoclonal
Myeloma Strain: P3X63-Ag.8-653
Epitope Mapped: No
Uniprot ID: Q9U617 
Immunogen Sequence: Total protein
Entrez Gene ID: 43930 
Additional Information: Pericardin is a type IV collagen-like ECM protein contributing to the ECM layer that surrounds the myocardial–pericardial network. EC1 1 was isolated by chance in a screen for monoclonal antibodies recognizing the muscle myosin heavy chain [PMID 3117889]. There are three Uniprot entries for this protein, two (Q7YU48 and Q9U617) are supported by experimental evidence at transcript level; the third, Q9VTR6, is a predicted protein; all three proteins are quite different; Q9U617 was selected as Uniprot ID because it encodes the NFQSTYYTK peptide identified in [PMID 12070098] by immunoprecipitation and sequencing. In [PMID 12070098], EC11 was used for immunogold on EM.
Antibody Registry ID: AB_528431 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
EC11 anti-Pericardin was deposited to the DSHB by Gratecos, Danielle (DSHB Hybridoma Product EC11 anti-Pericardin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication

    Cellular interactions during heart morphogenesis in the Drosophila embryo.
    Sémériva M
    Biology of the cell 84.1-2 (1995): 13-24.

    IF References

    Cellular interactions during heart morphogenesis in the Drosophila embryo.
    Sémériva M
    Biology of the cell 84.1-2 (1995): 13-24.

    Drosophila metalloproteases in development and differentiation: the role of ADAM proteins and their relatives.
    Paululat A
    European journal of cell biology 90.9 (2011 Sep): 770-8.

    A Drosophila model of high sugar diet-induced cardiomyopathy.
    Cagan R
    PLoS genetics 9.1 (2013): e1003175.

    Lateral positioning at the dorsal midline: Slit and Roundabout receptors guide Drosophila heart cell migration.
    Kramer SG
    Proceedings of the National Academy of Sciences of the United States of America 103.33 (2006 Aug 15): 12441-6.

    Pericardin, a Drosophila type IV collagen-like protein is involved in the morphogenesis and maintenance of the heart epithelium during dorsal ectoderm closure.
    Gratecos D
    Development (Cambridge, England) 129.13 (2002 Jul): 3241-53.

    The Drosophila homolog of vertebrate Islet1 is a key component in early cardiogenesis.
    Pandur P
    Development (Cambridge, England) 136.2 (2009 Jan): 317-26.

    GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis.
    Paululat A
    Journal of cell science 125.Pt 2 (2012 Jan 15): 461-72.

    Frazzled/DCC facilitates cardiac cell outgrowth and attachment during Drosophila dorsal vessel formation.
    Kramer SG
    Developmental biology 380.2 (2013 Aug 15): 233-42.

    The role of LamininB2 (LanB2) during mesoderm differentiation in Drosophila.
    Holz A
    Cellular and molecular life sciences : CMLS 69.2 (2012 Jan): 267-82.

    Phagocytic ability declines with age in adult Drosophila hemocytes.
    Starz-Gaiano M
    Aging cell 13.4 (2014 Aug): 719-28.

    Heart tube patterning in Drosophila requires integration of axial and segmental information provided by the Bithorax Complex genes and hedgehog signaling.
    Sémériva M
    Development (Cambridge, England) 129.19 (2002 Oct): 4509-21.

    A role for Drosophila Wnt-4 in heart development.
    Pandur P
    Genesis (New York, N.Y. : 2000) 50.6 (2012 Jun): 466-81.

    Drosophila cardiac tube organogenesis requires multiple phases of Hox activity.
    Semeriva M
    Developmental biology 272.2 (2004 Aug 15): 419-31.

    The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity.
    Paululat A
    PLoS genetics 9.7 (2013): e1003616.

    The bHLH transcription factor hand is required for proper wing heart formation in Drosophila.
    Paululat A
    Developmental biology 381.2 (2013 Sep 15): 446-59.

    The Iroquois complex is required in the dorsal mesoderm to ensure normal heart development in Drosophila.
    Pandur P
    PloS one 8.9 (2013): e76498.

    Distinct functions of the laminin β LN domain and collagen IV during cardiac extracellular matrix formation and stabilization of alary muscle attachments revealed by EMS mutagenesis in Drosophila.
    Reim I
    BMC developmental biology 14. (2014 Jun 17): 26.

    The heterotrimeric protein Go is required for the formation of heart epithelium in Drosophila.
    Sémériva M
    The Journal of cell biology 145.5 (1999 May 31): 1063-76.

    Distinct domains in the matricellular protein Lonely heart are crucial for cardiac extracellular matrix formation and heart function in <i>Drosophila</i>.
    Paululat A
    The Journal of biological chemistry 293.20 (2018 May 18): 7864-7879.

    WB References
    IHC References

    Cellular interactions during heart morphogenesis in the Drosophila embryo.
    Sémériva M
    Biology of the cell 84.1-2 (1995): 13-24.

    Loss of PTB or negative regulation of Notch mRNA reveals distinct zones of Notch and actin protein accumulation in Drosophila embryo.
    Wesley UV
    PloS one 6.7 (2011): e21876.

    The heterotrimeric protein Go is required for the formation of heart epithelium in Drosophila.
    Sémériva M
    The Journal of cell biology 145.5 (1999 May 31): 1063-76.

    Excessive Dpp signaling induces cardial apoptosis through dTAK1 and dJNK during late embryogenesis of Drosophila.
    Su MT
    Journal of biomedical science 18. (2011 Nov 24): 85.

    Causative role of oxidative stress in a Drosophila model of Friedreich ataxia.
    Moltó MD
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 21.2 (2007 Feb): 333-44.

    IP References
    All References

    Cellular interactions during heart morphogenesis in the Drosophila embryo.
    Sémériva M
    Biology of the cell 84.1-2 (1995): 13-24.

    Loss of PTB or negative regulation of Notch mRNA reveals distinct zones of Notch and actin protein accumulation in Drosophila embryo.
    Wesley UV
    PloS one 6.7 (2011): e21876.

    The heterotrimeric protein Go is required for the formation of heart epithelium in Drosophila.
    Sémériva M
    The Journal of cell biology 145.5 (1999 May 31): 1063-76.

    Excessive Dpp signaling induces cardial apoptosis through dTAK1 and dJNK during late embryogenesis of Drosophila.
    Su MT
    Journal of biomedical science 18. (2011 Nov 24): 85.

    Causative role of oxidative stress in a Drosophila model of Friedreich ataxia.
    Moltó MD
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 21.2 (2007 Feb): 333-44.

    Drosophila metalloproteases in development and differentiation: the role of ADAM proteins and their relatives.
    Paululat A
    European journal of cell biology 90.9 (2011 Sep): 770-8.

    A Drosophila model of high sugar diet-induced cardiomyopathy.
    Cagan R
    PLoS genetics 9.1 (2013): e1003175.

    Lateral positioning at the dorsal midline: Slit and Roundabout receptors guide Drosophila heart cell migration.
    Kramer SG
    Proceedings of the National Academy of Sciences of the United States of America 103.33 (2006 Aug 15): 12441-6.

    Pericardin, a Drosophila type IV collagen-like protein is involved in the morphogenesis and maintenance of the heart epithelium during dorsal ectoderm closure.
    Gratecos D
    Development (Cambridge, England) 129.13 (2002 Jul): 3241-53.

    The Drosophila homolog of vertebrate Islet1 is a key component in early cardiogenesis.
    Pandur P
    Development (Cambridge, England) 136.2 (2009 Jan): 317-26.

    GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis.
    Paululat A
    Journal of cell science 125.Pt 2 (2012 Jan 15): 461-72.

    Frazzled/DCC facilitates cardiac cell outgrowth and attachment during Drosophila dorsal vessel formation.
    Kramer SG
    Developmental biology 380.2 (2013 Aug 15): 233-42.

    The role of LamininB2 (LanB2) during mesoderm differentiation in Drosophila.
    Holz A
    Cellular and molecular life sciences : CMLS 69.2 (2012 Jan): 267-82.

    Phagocytic ability declines with age in adult Drosophila hemocytes.
    Starz-Gaiano M
    Aging cell 13.4 (2014 Aug): 719-28.

    Heart tube patterning in Drosophila requires integration of axial and segmental information provided by the Bithorax Complex genes and hedgehog signaling.
    Sémériva M
    Development (Cambridge, England) 129.19 (2002 Oct): 4509-21.

    A role for Drosophila Wnt-4 in heart development.
    Pandur P
    Genesis (New York, N.Y. : 2000) 50.6 (2012 Jun): 466-81.

    Drosophila cardiac tube organogenesis requires multiple phases of Hox activity.
    Semeriva M
    Developmental biology 272.2 (2004 Aug 15): 419-31.

    The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity.
    Paululat A
    PLoS genetics 9.7 (2013): e1003616.

    The bHLH transcription factor hand is required for proper wing heart formation in Drosophila.
    Paululat A
    Developmental biology 381.2 (2013 Sep 15): 446-59.

    The Iroquois complex is required in the dorsal mesoderm to ensure normal heart development in Drosophila.
    Pandur P
    PloS one 8.9 (2013): e76498.

    Distinct functions of the laminin β LN domain and collagen IV during cardiac extracellular matrix formation and stabilization of alary muscle attachments revealed by EMS mutagenesis in Drosophila.
    Reim I
    BMC developmental biology 14. (2014 Jun 17): 26.

    Distinct domains in the matricellular protein Lonely heart are crucial for cardiac extracellular matrix formation and heart function in <i>Drosophila</i>.
    Paululat A
    The Journal of biological chemistry 293.20 (2018 May 18): 7864-7879.

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