CF.2C7
Catalog Fields
Product Name/ID: CF.2C7
Available to For-Profits: Yes
Alternate Antibody Name: PS2, alphaPS2
Gene Name: if
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Entrez Gene ID: 32661
Clonality: Monoclonal
Immunogen: Sonicated imaginal discs
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: P3/NSI
Epitope Mapped: No
Antigen Name: integrin alphaPS2 (inflated)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/17/2002
Antigen Molecular Weight: Multiple isoforms
Depositor: Brower, D.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Drosophila
Depositor Notes: This antibody doesn't work for immunoblotting and is aldehyde sensitive.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Integrins are heterodimers of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain (resulting from the cleavage of the if gene product for alphaPS2) linked by a disulfide bond. AlphaPS2 associates with betaPS.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CF.2C7 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product CF.2C7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs.
Reger LA
Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.