CF.2C7

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$40.00
SKU: CF.2C7
View product citations for antibody CF.2C7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin alphaPS2 (inflated)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Brower, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody doesn't work for immunoblotting and is aldehyde sensitive.
Antigen Molecular Weight: Multiple isoforms
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: if
Immunogen: Sonicated imaginal discs
Alternate Gene Names: alphaPS2, CG9623
Alternate Antibody Name: PS2, alphaPS2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3/NSI
Epitope Location or Sequence:
Uniprot ID: P12080 
Immunogen Sequence: Total protein
Entrez Gene ID: 32661 
Additional Characterization:
Antibody Registry ID: AB_528304 
Additional Information: Integrins are heterodimers of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain (resulting from the cleavage of the if gene product for alphaPS2) linked by a disulfide bond. AlphaPS2 associates with betaPS.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CF.2C7 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product CF.2C7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FACS References
  • All References
  • Initial Publication

    Proceedings of the National Academy of Sciences of the United States of America
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    IF References

    Proceedings of the National Academy of Sciences of the United States of America
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    Developmental biology
    Salzberg A
    Developmental biology 351.1 (2011 Mar 1): 99-109.

    Biology open
    Geisbrecht ER
    Biology open 4.5 (2015 Apr 10): 636-48.

    The Journal of cell biology
    Horne-Badovinac S
    The Journal of cell biology 200.6 (2013 Mar 18): 721-9.

    Development (Cambridge, England)
    Zavortink M
    Development (Cambridge, England) 120.7 (1994 Jul): 1805-15.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Lemosy EK
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3927-39.

    Neuron
    Grueber WB
    Neuron 73.1 (2012 Jan 12): 79-91.

    Developmental biology
    Brown NH
    Developmental biology 308.2 (2007 Aug 15): 294-308.

    Genetics
    Brower DL
    Genetics 162.1 (2002 Sep): 285-96.

    Developmental biology
    Reger LA
    Developmental biology 108.1 (1985 Mar): 120-30.

    IHC References

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Chiba A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.19 (1998 Oct 1): 7847-55.

    IP References

    Developmental dynamics : an official publication of the American Association of Anatomists
    Fessler JH
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 116-28.

    Development (Cambridge, England)
    Bier E
    Development (Cambridge, England) 130.16 (2003 Aug): 3851-64.

    FACS References

    Molecular biology of the cell
    Shattil SJ
    Molecular biology of the cell 19.8 (2008 Aug): 3589-98.

    The Journal of biological chemistry
    Brower DL
    The Journal of biological chemistry 273.29 (1998 Jul 17): 18235-41.

    Developmental biology
    Brown NH
    Developmental biology 308.2 (2007 Aug 15): 294-308.

    All References

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Chiba A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.19 (1998 Oct 1): 7847-55.

    Proceedings of the National Academy of Sciences of the United States of America
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    Developmental biology
    Salzberg A
    Developmental biology 351.1 (2011 Mar 1): 99-109.

    Biology open
    Geisbrecht ER
    Biology open 4.5 (2015 Apr 10): 636-48.

    The Journal of cell biology
    Horne-Badovinac S
    The Journal of cell biology 200.6 (2013 Mar 18): 721-9.

    Development (Cambridge, England)
    Zavortink M
    Development (Cambridge, England) 120.7 (1994 Jul): 1805-15.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Lemosy EK
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3927-39.

    Neuron
    Grueber WB
    Neuron 73.1 (2012 Jan 12): 79-91.

    Developmental biology
    Brown NH
    Developmental biology 308.2 (2007 Aug 15): 294-308.

    Genetics
    Brower DL
    Genetics 162.1 (2002 Sep): 285-96.

    Developmental biology
    Reger LA
    Developmental biology 108.1 (1985 Mar): 120-30.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Fessler JH
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 116-28.

    Development (Cambridge, England)
    Bier E
    Development (Cambridge, England) 130.16 (2003 Aug): 3851-64.

    Molecular biology of the cell
    Shattil SJ
    Molecular biology of the cell 19.8 (2008 Aug): 3589-98.

    The Journal of biological chemistry
    Brower DL
    The Journal of biological chemistry 273.29 (1998 Jul 17): 18235-41.

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