CF.2C7

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SKU: CF.2C7

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DSHB Data Sheet

Catalog Fields

Product Name/ID: CF.2C7
Available to For-Profits: Yes
Alternate Antibody Name: PS2, alphaPS2
Gene Name: if
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528304 
Uniprot ID: P12080 
RRID:  
Entrez Gene ID: 32661 
Clonality: Monoclonal
Immunogen: Sonicated imaginal discs
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: P3/NSI
Epitope Mapped: No
Antigen Name: integrin alphaPS2 (inflated)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/17/2002
Antigen Molecular Weight: Multiple isoforms
Depositor: Brower, D.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Drosophila
Depositor Notes: This antibody doesn't work for immunoblotting and is aldehyde sensitive.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Integrins are heterodimers of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain (resulting from the cleavage of the if gene product for alphaPS2) linked by a disulfide bond. AlphaPS2 associates with betaPS.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CF.2C7 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product CF.2C7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

15 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FACS References
  • All References
  • Initial Publication

    Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs.
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    IF References

    Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs.
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression.
    Salzberg A
    Developmental biology 351.1 (2011 Mar 1): 99-109.

    Loss of a Clueless-dGRASP complex results in ER stress and blocks Integrin exit from the perinuclear endoplasmic reticulum in Drosophila larval muscle.
    Geisbrecht ER
    Biology open 4.5 (2015 Apr 10): 636-48.

    Misshapen decreases integrin levels to promote epithelial motility and planar polarity in Drosophila.
    Horne-Badovinac S
    The Journal of cell biology 200.6 (2013 Mar 18): 721-9.

    The role of apterous in the control of dorsoventral compartmentalization and PS integrin gene expression in the developing wing of Drosophila.
    Zavortink M
    Development (Cambridge, England) 120.7 (1994 Jul): 1805-15.

    Integrin alpha chains exhibit distinct temporal and spatial localization patterns in epithelial cells of the Drosophila ovary.
    Lemosy EK
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3927-39.

    Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons.
    Grueber WB
    Neuron 73.1 (2012 Jan 12): 79-91.

    Mutations in the Drosophila alphaPS2 integrin subunit uncover new features of adhesion site assembly.
    Brown NH
    Developmental biology 308.2 (2007 Aug 15): 294-308.

    Genetic interaction between integrins and moleskin, a gene encoding a Drosophila homolog of importin-7.
    Brower DL
    Genetics 162.1 (2002 Sep): 285-96.

    Developmental analysis of Drosophila position-specific antigens.
    Reger LA
    Developmental biology 108.1 (1985 Mar): 120-30.

    IHC References

    Genetic analysis on the role of integrin during axon guidance in Drosophila.
    Chiba A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.19 (1998 Oct 1): 7847-55.

    IP References

    Differentiation, extracellular matrix synthesis, and integrin assembly by Drosophila embryo cells cultured on vitronectin and laminin substrates.
    Fessler JH
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 116-28.

    Integrins modulate Sog activity in the Drosophila wing.
    Bier E
    Development (Cambridge, England) 130.16 (2003 Aug): 3851-64.

    FACS References
    All References

    Genetic analysis on the role of integrin during axon guidance in Drosophila.
    Chiba A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.19 (1998 Oct 1): 7847-55.

    Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs.
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression.
    Salzberg A
    Developmental biology 351.1 (2011 Mar 1): 99-109.

    Loss of a Clueless-dGRASP complex results in ER stress and blocks Integrin exit from the perinuclear endoplasmic reticulum in Drosophila larval muscle.
    Geisbrecht ER
    Biology open 4.5 (2015 Apr 10): 636-48.

    Misshapen decreases integrin levels to promote epithelial motility and planar polarity in Drosophila.
    Horne-Badovinac S
    The Journal of cell biology 200.6 (2013 Mar 18): 721-9.

    The role of apterous in the control of dorsoventral compartmentalization and PS integrin gene expression in the developing wing of Drosophila.
    Zavortink M
    Development (Cambridge, England) 120.7 (1994 Jul): 1805-15.

    Integrin alpha chains exhibit distinct temporal and spatial localization patterns in epithelial cells of the Drosophila ovary.
    Lemosy EK
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3927-39.

    Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons.
    Grueber WB
    Neuron 73.1 (2012 Jan 12): 79-91.

    Mutations in the Drosophila alphaPS2 integrin subunit uncover new features of adhesion site assembly.
    Brown NH
    Developmental biology 308.2 (2007 Aug 15): 294-308.

    Genetic interaction between integrins and moleskin, a gene encoding a Drosophila homolog of importin-7.
    Brower DL
    Genetics 162.1 (2002 Sep): 285-96.

    Developmental analysis of Drosophila position-specific antigens.
    Reger LA
    Developmental biology 108.1 (1985 Mar): 120-30.

    Differentiation, extracellular matrix synthesis, and integrin assembly by Drosophila embryo cells cultured on vitronectin and laminin substrates.
    Fessler JH
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 116-28.

    Integrins modulate Sog activity in the Drosophila wing.
    Bier E
    Development (Cambridge, England) 130.16 (2003 Aug): 3851-64.

    Differences in regulation of Drosophila and vertebrate integrin affinity by talin.
    Shattil SJ
    Molecular biology of the cell 19.8 (2008 Aug): 3589-98.

    Splice variants of the Drosophila PS2 integrins differentially interact with RGD-containing fragments of the extracellular proteins tiggrin, ten-m, and D-laminin 2.
    Brower DL
    The Journal of biological chemistry 273.29 (1998 Jul 17): 18235-41.

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