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View product citations for antibody KLAR-M 4B5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: klarsicht, exon 9
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Wieschaus, E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University
Positive Tested Species Reactivity: Drosophila melanogaster
Depositors Notes: mutant analysis indicates that it specifically recognizes Klar by western blotting and immunohistochemistry. Works well with heat-fixed tissues.
Antigen Molecular Weight: Predicted: multiple isoforms from 56 to 246 kDa; Apparent: major band above 250 kDa and multiple additional bands.
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: klar
Immunogen: Recombinant GST/Klarsicht (isoform A, aa 875-1169) fusion protein
Alternate Gene Names: CG17046, kls, marb
Alternate Antibody Name: KLAR-M
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: aa 875-1169 of isoform A
Uniprot ID: Q9W0Q4 
Immunogen Sequence: Partial protein
Entrez Gene ID: 38067 
Additional Characterization:  
Antibody Registry ID: AB_10573114 
Additional Information: KLAR-M should recognize isoformsA, B, D, E, F, and H (not C, G, or I) [isoform nomenclature from FlyBase].
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
KLAR-M 4B5 was deposited to the DSHB by Wieschaus, E. (DSHB Hybridoma Product KLAR-M 4B5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Organelle-specific control of intracellular transport: distinctly targeted isoforms of the regulator Klar.
    Welte MA
    Molecular biology of the cell 16.3 (2005 Mar): 1406-16.

    Klar ensures thermal robustness of oskar localization by restraining RNP motility.
    Welte MA
    The Journal of cell biology 206.2 (2014 Jul 21): 199-215.

    Novel isoforms of the transport regulator klar.
    Welte M
    PloS one 8.2 (2013): e55070.

    Organelle positioning in muscles requires cooperation between two KASH proteins and microtubules.
    Volk T
    The Journal of cell biology 198.5 (2012 Sep 3): 833-46.

    Targeting the motor regulator Klar to lipid droplets.
    Welte MA
    BMC cell biology 12. (2011 Feb 24): 9.

    Epitope Map References
    All References

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