2C11-2

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$40.00
SKU: 2C11-2
View product citations for antibody 2C11-2 on CiteAb

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Available: 85

DSHB Data Sheet

Catalog Fields

Antigen: Dynein heavy chain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Scholey, J.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Davis
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Dhc
Immunogen: preparation of Drosophila microtubule-associated proteins
Alternate Gene Names: dynein heavy chain, Dhc64C
Alternate Antibody Name: anti-DHC mAb alpha DHC mouse anti-Dhc
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: P37276 
Immunogen Sequence:
Entrez Gene ID: 38580 
Additional Characterization:  
Antibody Registry ID: AB_2091523 
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2C11-2 was deposited to the DSHB by Scholey, J.M. (DSHB Hybridoma Product 2C11-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication

    Functional coordination of three mitotic motors in Drosophila embryos.
    Scholey JM
    Molecular biology of the cell 11.1 (2000 Jan): 241-53.

    IF References
    WB References
    IHC References
    IP References
    FB References

    Functional coordination of three mitotic motors in Drosophila embryos.
    Scholey JM
    Molecular biology of the cell 11.1 (2000 Jan): 241-53.

    Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos.
    Scholey JM
    Nature cell biology 2.12 (2000 Dec): 922-30.

    All References

    Functional coordination of three mitotic motors in Drosophila embryos.
    Scholey JM
    Molecular biology of the cell 11.1 (2000 Jan): 241-53.

    Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos.
    Scholey JM
    Nature cell biology 2.12 (2000 Dec): 922-30.

    The golgin Lava lamp mediates dynein-based Golgi movements during Drosophila cellularization.
    Sisson JC
    Nature cell biology 7.6 (2005 Jun): 612-8.

    The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline.
    Palacios IM
    Development (Cambridge, England) 141.1 (2014 Jan): 176-86.

    A genetic screen based on in vivo RNA imaging reveals centrosome-independent mechanisms for localizing gurken transcripts in Drosophila.
    Ish-Horowicz D
    G3 (Bethesda, Md.) 4.4 (2014 Apr 16): 749-60.

    Drosophila Syd-1, liprin-α, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons.
    Wu C
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.25 (2014 Jun 18): 8474-87.

    Lipid droplets purified from Drosophila embryos as an endogenous handle for precise motor transport measurements.
    Shubeita GT
    Biophysical journal 105.5 (2013 Sep 3): 1182-91.

    Opposite-polarity motors activate one another to trigger cargo transport in live cells.
    Gelfand VI
    The Journal of cell biology 187.7 (2009 Dec 28): 1071-82.

    Microtubule binding by dynactin is required for microtubule organization but not cargo transport.
    Gelfand VI
    The Journal of cell biology 176.5 (2007 Feb 26): 641-51.

    Single-molecule assays reveal that RNA localization signals regulate dynein-dynactin copy number on individual transcript cargoes.
    Bullock SL
    Nature cell biology 14.4 (2012 Feb 26): 416-23.

    Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.
    Bullock SL
    The Journal of cell biology 202.3 (2013 Aug 5): 479-94.

    Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to coordinate recruitment of cargos to dynein.
    Bullock SL
    Genes & development 27.11 (2013 Jun 1): 1233-46.

    FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism.
    Darnell RB
    Cell 146.2 (2011 Jul 22): 247-61.

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