MAb83F6
Catalog Fields
Antigen: Transient receptor potential protein (TRP)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology, Division of Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Overfixation reduces the efficacy of MAb83F6
Predicted Species Reactivity:
Gene: trp
Immunogen: Purified rhabdomeres
Alternate Gene Names: CG7875, 559
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Intracellular C-terminus
Uniprot ID: P19334
Immunogen Sequence: Full length protein
Entrez Gene ID: 43542
Antibody Registry ID: AB_528496
Additional Information: Doesn't stain null trp fly mutants. trp is a transmembrane light-sensitive calcium channel required for phototransduction. RRID: AB_528496. FBgn0003861
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MAb83F6 was deposited to the DSHB by Benzer, S. (DSHB Hybridoma Product MAb83F6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
18 References
Initial Publication
TRP, a protein essential for inositide-mediated Ca2+ influx is localized adjacent to the calcium stores in Drosophila photoreceptors.
Minke B
The Journal of neuroscience : the official journal of the Society for Neuroscience 15.5 Pt 2 (1995 May): 3747-60.