MAb83F6

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$40.00
SKU: MAb83F6
View product citations for antibody MAb83F6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Transient receptor potential protein (TRP)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology, Division of Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Overfixation reduces the efficacy of MAb83F6
Antigen Molecular Weight: 143 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: trp
Immunogen: Purified rhabdomeres
Alternate Gene Names: CG7875, 559
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Intracellular C-terminus
Uniprot ID: P19334 
Immunogen Sequence: Full length protein
Entrez Gene ID: 43542 
Additional Characterization:  
Antibody Registry ID: AB_528496 
Additional Information: Doesn't stain null trp fly mutants. trp is a transmembrane light-sensitive calcium channel required for phototransduction. RRID: AB_528496. FBgn0003861
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MAb83F6 was deposited to the DSHB by Benzer, S. (DSHB Hybridoma Product MAb83F6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    TRP, a protein essential for inositide-mediated Ca2+ influx is localized adjacent to the calcium stores in Drosophila photoreceptors.
    Minke B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.5 Pt 2 (1995 May): 3747-60.

    IF References

    TRP, a protein essential for inositide-mediated Ca2+ influx is localized adjacent to the calcium stores in Drosophila photoreceptors.
    Minke B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.5 Pt 2 (1995 May): 3747-60.

    Light-dependent phosphorylation of the drosophila transient receptor potential ion channel.
    Huber A
    The Journal of biological chemistry 285.19 (2010 May 7): 14275-84.

    Drosophila TRP channels require a protein with a distinctive motif encoded by the inaF locus.
    Nash HA
    Proceedings of the National Academy of Sciences of the United States of America 104.45 (2007 Nov 6): 17730-4.

    Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo.
    Huber A
    Journal of cell science 126.Pt 14 (2013 Jul 15): 3121-33.

    Phosphorylation of the Drosophila transient receptor potential ion channel is regulated by the phototransduction cascade and involves several protein kinases and phosphatases.
    Huber A
    PloS one 8.9 (2013): e73787.

    The GTP- and Phospholipid-Binding Protein TTD14 Regulates Trafficking of the TRPL Ion Channel in Drosophila Photoreceptor Cells.
    Huber A
    PLoS genetics 11.10 (2015 Oct): e1005578.

    Immunocytochemical Labeling of Rhabdomeric Proteins in <i>Drosophila</i> Photoreceptor Cells Is Compromised by a Light-dependent Technical Artifact.
    Huber A
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 67.10 (2019 Oct): 745-757.

    Functional characterization of the three Drosophila retinal degeneration C (RDGC) protein phosphatase isoforms.
    Huber A
    PloS one 13.9 (2018): e0204933.

    Solubility and subcellular localization of the three Drosophila RDGC phosphatase variants are determined by acylation.
    Voolstra O
    FEBS letters 592.14 (2018 Jul): 2403-2413.

    The latency of the light response is modulated by the phosphorylation state of Drosophila TRP at a specific site.
    Minke B
    Channels (Austin, Tex.) 11.6 (2017 Nov 2): 678-685.

    The GTP- and Phospholipid-Binding Protein TTD14 Regulates Trafficking of the TRPL Ion Channel in Drosophila Photoreceptor Cells.
    Huber A
    PLoS genetics 11.10 (2015 Oct): e1005578.

    TRP, TRPL and cacophony channels mediate Ca2+ influx and exocytosis in photoreceptors axons in Drosophila.
    Bacigalupo J
    PloS one 7.8 (2012): e44182.

    WB References

    TRP, a protein essential for inositide-mediated Ca2+ influx is localized adjacent to the calcium stores in Drosophila photoreceptors.
    Minke B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.5 Pt 2 (1995 May): 3747-60.

    Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca2+ entry.
    Minke B
    Proceedings of the National Academy of Sciences of the United States of America 93.24 (1996 Nov 26): 14146-51.

    Membrane lipid modulations remove divalent open channel block from TRP-like and NMDA channels.
    Minke B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.8 (2009 Feb 25): 2371-83.

    Light-dependent phosphorylation of the drosophila transient receptor potential ion channel.
    Huber A
    The Journal of biological chemistry 285.19 (2010 May 7): 14275-84.

    Drosophila TRP channels require a protein with a distinctive motif encoded by the inaF locus.
    Nash HA
    Proceedings of the National Academy of Sciences of the United States of America 104.45 (2007 Nov 6): 17730-4.

    Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo.
    Huber A
    Journal of cell science 126.Pt 14 (2013 Jul 15): 3121-33.

    Translocation of the Drosophila transient receptor potential-like (TRPL) channel requires both the N- and C-terminal regions together with sustained Ca2+ entry.
    Huber A
    The Journal of biological chemistry 286.39 (2011 Sep 30): 34234-43.

    An eGFP-based genetic screen for defects in light-triggered subcelluar translocation of the Drosophila photoreceptor channel TRPL.
    Huber A
    Fly 2.1 (2008 Jan-Feb): 36-46.

    A forward genetic screen in Drosophila melanogaster to identify mutations affecting INAD localization in photoreceptor cells.
    Tsunoda S
    Fly 4.2 (2010 Apr-Jun): 95-103.

    The GTP- and Phospholipid-Binding Protein TTD14 Regulates Trafficking of the TRPL Ion Channel in Drosophila Photoreceptor Cells.
    Huber A
    PLoS genetics 11.10 (2015 Oct): e1005578.

    The latency of the light response is modulated by the phosphorylation state of Drosophila TRP at a specific site.
    Minke B
    Channels (Austin, Tex.) 11.6 (2017 Nov 2): 678-685.

    IHC References
    IP References

    Light-dependent phosphorylation of the drosophila transient receptor potential ion channel.
    Huber A
    The Journal of biological chemistry 285.19 (2010 May 7): 14275-84.

    Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo.
    Huber A
    Journal of cell science 126.Pt 14 (2013 Jul 15): 3121-33.

    Epitope Map References

    Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo.
    Huber A
    Journal of cell science 126.Pt 14 (2013 Jul 15): 3121-33.

    All References

    Multilevel regulation of the glass locus during Drosophila eye development.
    Sprecher SG
    PLoS genetics 15.7 (2019 Jul): e1008269.

    Lysosomal Machinery Drives Extracellular Acidification to Direct Non-apoptotic Cell Death.
    McCall K
    Cell reports 27.1 (2019 Apr 2): 11-19.e3.

    TRP, a protein essential for inositide-mediated Ca2+ influx is localized adjacent to the calcium stores in Drosophila photoreceptors.
    Minke B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.5 Pt 2 (1995 May): 3747-60.

    Light-dependent phosphorylation of the drosophila transient receptor potential ion channel.
    Huber A
    The Journal of biological chemistry 285.19 (2010 May 7): 14275-84.

    Drosophila TRP channels require a protein with a distinctive motif encoded by the inaF locus.
    Nash HA
    Proceedings of the National Academy of Sciences of the United States of America 104.45 (2007 Nov 6): 17730-4.

    Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo.
    Huber A
    Journal of cell science 126.Pt 14 (2013 Jul 15): 3121-33.

    Phosphorylation of the Drosophila transient receptor potential ion channel is regulated by the phototransduction cascade and involves several protein kinases and phosphatases.
    Huber A
    PloS one 8.9 (2013): e73787.

    The GTP- and Phospholipid-Binding Protein TTD14 Regulates Trafficking of the TRPL Ion Channel in Drosophila Photoreceptor Cells.
    Huber A
    PLoS genetics 11.10 (2015 Oct): e1005578.

    Immunocytochemical Labeling of Rhabdomeric Proteins in <i>Drosophila</i> Photoreceptor Cells Is Compromised by a Light-dependent Technical Artifact.
    Huber A
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 67.10 (2019 Oct): 745-757.

    Functional characterization of the three Drosophila retinal degeneration C (RDGC) protein phosphatase isoforms.
    Huber A
    PloS one 13.9 (2018): e0204933.

    Solubility and subcellular localization of the three Drosophila RDGC phosphatase variants are determined by acylation.
    Voolstra O
    FEBS letters 592.14 (2018 Jul): 2403-2413.

    The latency of the light response is modulated by the phosphorylation state of Drosophila TRP at a specific site.
    Minke B
    Channels (Austin, Tex.) 11.6 (2017 Nov 2): 678-685.

    TRP, TRPL and cacophony channels mediate Ca2+ influx and exocytosis in photoreceptors axons in Drosophila.
    Bacigalupo J
    PloS one 7.8 (2012): e44182.

    Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca2+ entry.
    Minke B
    Proceedings of the National Academy of Sciences of the United States of America 93.24 (1996 Nov 26): 14146-51.

    Membrane lipid modulations remove divalent open channel block from TRP-like and NMDA channels.
    Minke B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.8 (2009 Feb 25): 2371-83.

    Translocation of the Drosophila transient receptor potential-like (TRPL) channel requires both the N- and C-terminal regions together with sustained Ca2+ entry.
    Huber A
    The Journal of biological chemistry 286.39 (2011 Sep 30): 34234-43.

    An eGFP-based genetic screen for defects in light-triggered subcelluar translocation of the Drosophila photoreceptor channel TRPL.
    Huber A
    Fly 2.1 (2008 Jan-Feb): 36-46.

    A forward genetic screen in Drosophila melanogaster to identify mutations affecting INAD localization in photoreceptor cells.
    Tsunoda S
    Fly 4.2 (2010 Apr-Jun): 95-103.

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