Antigen: Nervana; Sodium/potassium-transporting ATPase subunit beta-1
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Salvaterra, P.M.
Host Species: mouse
Depositors Institution: Beckman Research Institute of the City of Hope
Positive Tested Species Reactivity: Drosophila, Insects
Depositors Notes: Nervana is one of several components of the Drosophila nervous system that is recognized by horseradish peroxidase antibodies.
Predicted Species Reactivity:
Immunogen: Brain glycoproteins purified by lentil lectin binding and HRP reactivity
Alternate Gene Names: Dmel_CG9258
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q24046
Immunogen Sequence: Full length sequence
Entrez Gene ID: 33952
Antibody Registry ID: AB_528395
Additional Information: RRID: AB_528395 FBgn0015776.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Nrv5F7 was deposited to the DSHB by Salvaterra, P.M. (DSHB Hybridoma Product Nrv5F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.