Nrv5F7

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$40.00
SKU: Nrv5F7-s
View product citations for antibody Nrv5F7 on CiteAb

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Available: 98

DSHB Data Sheet

Catalog Fields

Antigen: Nervana; Sodium/potassium-transporting ATPase subunit beta-1
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Salvaterra, P.M.
Isotype: MIgG1
Depositors Institution: Beckman Research Institute of the City of Hope
Host Species: mouse
Depositors Notes: Nervana is one of several components of the Drosophila nervous system that is recognized by horseradish peroxidase antibodies.
Positive Tested Species Reactivity: Drosophila, Insects
Gene: nrv1
Antigen Molecular Weight: 35.3 kDa
Alternate Gene Names: Dmel_CG9258
Immunogen: Brain glycoproteins purified by lentil lectin binding and HRP reactivity
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: No
Uniprot ID: Q24046 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 33952 
Additional Information: RRID: AB_528395 FBgn0015776.
Antibody Registry ID: AB_528395 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Nrv5F7 was deposited to the DSHB by Salvaterra, P.M. (DSHB Hybridoma Product Nrv5F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants.
    Dobler S
    Evolution; international journal of organic evolution 70.12 (2016 Dec): 2767-2777.

    Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation.
    Yamaguchi M
    Nucleic acids research 45.3 (2017 Feb 17): 1233-1254.

    Select Septate Junction Proteins Direct ROS-Mediated Paracrine Regulation of Drosophila Cardiac Function.
    Wang W
    Cell reports 28.6 (2019 Aug 6): 1455-1470.e4.

    WB References

    Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants.
    Dobler S
    Evolution; international journal of organic evolution 70.12 (2016 Dec): 2767-2777.

    Dual Roles for Membrane Association of Drosophila Axin in Wnt Signaling.
    Ahmed Y
    PLoS genetics 12.12 (2016 Dec): e1006494.

    IHC References

    Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants.
    Dobler S
    Evolution; international journal of organic evolution 70.12 (2016 Dec): 2767-2777.

    All References

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