Nrv5F7

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$45.00
SKU: Nrv5F7-s
View product citations for antibody Nrv5F7 on CiteAb

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Available: 49

DSHB Data Sheet

Catalog Fields

Antigen: Nervana; Sodium/potassium-transporting ATPase subunit beta-1
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Salvaterra, P.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: City of Hope, Beckman Research Institute
Positive Tested Species Reactivity: Drosophila, Insects
Depositors Notes: Nervana is one of several components of the Drosophila nervous system that is recognized by horseradish peroxidase antibodies.
Antigen Molecular Weight: 35.3 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: nrv1
Immunogen: Brain glycoproteins purified by lentil lectin binding and HRP reactivity
Alternate Gene Names: Dmel_CG9258
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q24046 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 33952 
Additional Characterization:  
Antibody Registry ID: AB_528395 
Additional Information: RRID: AB_528395 FBgn0015776.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Nrv5F7 was deposited to the DSHB by Salvaterra, P.M. (DSHB Hybridoma Product Nrv5F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants.
    Dobler S
    Evolution; international journal of organic evolution 70.12 (2016 Dec): 2767-2777.

    Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation.
    Yamaguchi M
    Nucleic acids research 45.3 (2017 Feb 17): 1233-1254.

    Select Septate Junction Proteins Direct ROS-Mediated Paracrine Regulation of Drosophila Cardiac Function.
    Wang W
    Cell reports 28.6 (2019 Aug 6): 1455-1470.e4.

    WB References

    Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants.
    Dobler S
    Evolution; international journal of organic evolution 70.12 (2016 Dec): 2767-2777.

    Dual Roles for Membrane Association of Drosophila Axin in Wnt Signaling.
    Ahmed Y
    PLoS genetics 12.12 (2016 Dec): e1006494.

    IHC References

    Gene duplications circumvent trade-offs in enzyme function: Insect adaptation to toxic host plants.
    Dobler S
    Evolution; international journal of organic evolution 70.12 (2016 Dec): 2767-2777.

    All References

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