PDF C7

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DSHB Data Sheet

Catalog Fields

Antigen: Pigment-dispersing factor neuropeptide
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Blau, Justin
Isotype: MIgG2b, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Biology, New York University
Positive Tested Species Reactivity: Cabbage root fly, Cockroach, Drosophila
Depositors Notes: Fusion date: 2005. Recognizes Drosophila larval and adult brains.
Antigen Molecular Weight: Predicted: PDF, 2kDa; PDF precursor, 11.5kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Pdf
Immunogen: amidated pigment dispersing factor neuropeptide
Alternate Gene Names: Dmel\CG6496; Drm-pdf; Drm-PDF; PAP; cPDH; PDH
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2-0/Ag14
Epitope Location or Sequence: NSELINSLLSLPKNMNDA-NH2
Uniprot ID: O96690 
Immunogen Sequence: NSELINSLLSLPKNMNDA-NH2
Entrez Gene ID: 43193 
Additional Characterization:  
Antibody Registry ID: AB_760350 AB_2315084 
Additional Information: The neuropeptide PDF represents the C-terminus portion (aa 83-100) of the Protein PDF [Uniprot O96690]. The neuropeptide PDF regulates circadian loco motor rhythms.
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PDF C7 was deposited to the DSHB by Blau, Justin (DSHB Hybridoma Product PDF C7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

34 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    The double-time protein kinase regulates the subcellular localization of the Drosophila clock protein period.
    Blau J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 25.22 (2005 Jun 1): 5430-7.

    IF References

    Differentially timed extracellular signals synchronize pacemaker neuron clocks.
    Blau J
    PLoS biology 12.9 (2014 Sep): e1001959.

    Identification of a circadian output circuit for rest:activity rhythms in Drosophila.
    Sehgal A
    Cell 157.3 (2014 Apr 24): 689-701.

    Dual PDF signaling pathways reset clocks via TIMELESS and acutely excite target neurons to control circadian behavior.
    Allada R
    PLoS biology 12.3 (2014 Mar): e1001810.

    Activity-dependent regulation of astrocyte GAT levels during synaptogenesis.
    Freeman MR
    Nature neuroscience 17.10 (2014 Oct): 1340-50.

    The ion transport peptide is a new functional clock neuropeptide in the fruit fly Drosophila melanogaster.
    Helfrich-Förster C
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.29 (2014 Jul 16): 9522-36.

    The logic of circadian organization in Drosophila.
    Rosato E
    Current biology : CB 24.19 (2014 Oct 6): 2257-66.

    Casein kinase 1 promotes synchrony of the circadian clock network.
    Sehgal A
    Molecular and cellular biology 34.14 (2014 Jul): 2682-94.

    The central molecular clock is robust in the face of behavioural arrhythmia in a Drosophila model of Alzheimer's disease.
    Crowther DC
    Disease models & mechanisms 7.4 (2014 Apr): 445-58.

    Myoinhibitory peptides in the brain of the cockroach Leucophaea maderae and colocalization with pigment-dispersing factor in circadian pacemaker cells.
    Stengl M
    The Journal of comparative neurology 520.5 (2012 Apr 1): 1078-97.

    Adult-specific electrical silencing of pacemaker neurons uncouples molecular clock from circadian outputs.
    Ceriani MF
    Current biology : CB 21.21 (2011 Nov 8): 1783-93.

    Circadian pacemaker coupling by multi-peptidergic neurons in the cockroach Leucophaea maderae.
    Reischig T
    Cell and tissue research 343.3 (2011 Mar): 559-77.

    Distinct visual pathways mediate Drosophila larval light avoidance and circadian clock entrainment.
    Sprecher SG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.17 (2011 Apr 27): 6527-34.

    Flies in the north: locomotor behavior and clock neuron organization of Drosophila montana.
    Hoikkala A
    Journal of biological rhythms 27.5 (2012 Oct): 377-87.

    GABA(B) receptors play an essential role in maintaining sleep during the second half of the night in Drosophila melanogaster.
    Helfrich-Förster C
    The Journal of experimental biology 216.Pt 20 (2013 Oct 15): 3837-43.

    The circadian clock network in the brain of different Drosophila species.
    Helfrich-Förster C
    The Journal of comparative neurology 521.2 (2013 Feb 1): 367-88.

    CULLIN-3 controls TIMELESS oscillations in the Drosophila circadian clock.
    Rouyer F
    PLoS biology 10.8 (2012): e1001367.

    Adult circadian behavior in Drosophila requires developmental expression of cycle, but not period.
    Wijnen H
    PLoS genetics 7.7 (2011 Jul): e1002167.

    Peptidomics of the agriculturally damaging larval stage of the cabbage root fly Delia radicum (Diptera: Anthomyiidae).
    Wegener C
    PloS one 7.7 (2012): e41543.

    Sensorimotor structure of Drosophila larva phototaxis.
    Samuel AD
    Proceedings of the National Academy of Sciences of the United States of America 110.40 (2013 Oct 1): E3868-77.

    Synchronized bilateral synaptic inputs to Drosophila melanogaster neuropeptidergic rest/arousal neurons.
    Nitabach MN
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.22 (2011 Jun 1): 8181-93.

    A role for O-GlcNAcylation in setting circadian clock speed.
    Cho JW
    Genes & development 26.5 (2012 Mar 1): 490-502.

    Drosophila spaghetti and doubletime link the circadian clock and light to caspases, apoptosis and tauopathy.
    Price JL
    PLoS genetics 11.5 (2015 May): e1005171.

    The Drosophila larval visual system: high-resolution analysis of a simple visual neuropil.
    Hartenstein V
    Developmental biology 358.1 (2011 Oct 1): 33-43.

    Using Drosophila as an integrated model to study mild repetitive traumatic brain injury.
    Ratliff EP
    Scientific reports 6. (2016 May 4): 25252.

    Fbxl4 Serves as a Clock Output Molecule that Regulates Sleep through Promotion of Rhythmic Degradation of the GABA<sub>A</sub> Receptor.
    Han J
    Current biology : CB 27.23 (2017 Dec 4): 3616-3625.e5.

    The neuropeptide SIFamide in the brain of three cockroach species.
    Stengl M
    The Journal of comparative neurology 524.7 (2016 May 1): 1337-60.

    IHC References

    Glutathione peroxidase activity is neuroprotective in models of Huntington's disease.
    Giorgini F
    Nature genetics 45.10 (2013 Oct): 1249-54.

    Mushroom body signaling is required for locomotor activity rhythms in Drosophila.
    Sakai T
    Neuroscience research 111. (2016 Oct): 25-33.

    The neuropeptide SIFamide in the brain of three cockroach species.
    Stengl M
    The Journal of comparative neurology 524.7 (2016 May 1): 1337-60.

    Epitope Map References

    The double-time protein kinase regulates the subcellular localization of the Drosophila clock protein period.
    Blau J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 25.22 (2005 Jun 1): 5430-7.

    All References

    Glutathione peroxidase activity is neuroprotective in models of Huntington's disease.
    Giorgini F
    Nature genetics 45.10 (2013 Oct): 1249-54.

    Mushroom body signaling is required for locomotor activity rhythms in Drosophila.
    Sakai T
    Neuroscience research 111. (2016 Oct): 25-33.

    The neuropeptide SIFamide in the brain of three cockroach species.
    Stengl M
    The Journal of comparative neurology 524.7 (2016 May 1): 1337-60.

    Differentially timed extracellular signals synchronize pacemaker neuron clocks.
    Blau J
    PLoS biology 12.9 (2014 Sep): e1001959.

    Identification of a circadian output circuit for rest:activity rhythms in Drosophila.
    Sehgal A
    Cell 157.3 (2014 Apr 24): 689-701.

    Dual PDF signaling pathways reset clocks via TIMELESS and acutely excite target neurons to control circadian behavior.
    Allada R
    PLoS biology 12.3 (2014 Mar): e1001810.

    Activity-dependent regulation of astrocyte GAT levels during synaptogenesis.
    Freeman MR
    Nature neuroscience 17.10 (2014 Oct): 1340-50.

    The ion transport peptide is a new functional clock neuropeptide in the fruit fly Drosophila melanogaster.
    Helfrich-Förster C
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.29 (2014 Jul 16): 9522-36.

    The logic of circadian organization in Drosophila.
    Rosato E
    Current biology : CB 24.19 (2014 Oct 6): 2257-66.

    Casein kinase 1 promotes synchrony of the circadian clock network.
    Sehgal A
    Molecular and cellular biology 34.14 (2014 Jul): 2682-94.

    The central molecular clock is robust in the face of behavioural arrhythmia in a Drosophila model of Alzheimer's disease.
    Crowther DC
    Disease models & mechanisms 7.4 (2014 Apr): 445-58.

    Myoinhibitory peptides in the brain of the cockroach Leucophaea maderae and colocalization with pigment-dispersing factor in circadian pacemaker cells.
    Stengl M
    The Journal of comparative neurology 520.5 (2012 Apr 1): 1078-97.

    Adult-specific electrical silencing of pacemaker neurons uncouples molecular clock from circadian outputs.
    Ceriani MF
    Current biology : CB 21.21 (2011 Nov 8): 1783-93.

    Circadian pacemaker coupling by multi-peptidergic neurons in the cockroach Leucophaea maderae.
    Reischig T
    Cell and tissue research 343.3 (2011 Mar): 559-77.

    Distinct visual pathways mediate Drosophila larval light avoidance and circadian clock entrainment.
    Sprecher SG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.17 (2011 Apr 27): 6527-34.

    Flies in the north: locomotor behavior and clock neuron organization of Drosophila montana.
    Hoikkala A
    Journal of biological rhythms 27.5 (2012 Oct): 377-87.

    GABA(B) receptors play an essential role in maintaining sleep during the second half of the night in Drosophila melanogaster.
    Helfrich-Förster C
    The Journal of experimental biology 216.Pt 20 (2013 Oct 15): 3837-43.

    The circadian clock network in the brain of different Drosophila species.
    Helfrich-Förster C
    The Journal of comparative neurology 521.2 (2013 Feb 1): 367-88.

    CULLIN-3 controls TIMELESS oscillations in the Drosophila circadian clock.
    Rouyer F
    PLoS biology 10.8 (2012): e1001367.

    Adult circadian behavior in Drosophila requires developmental expression of cycle, but not period.
    Wijnen H
    PLoS genetics 7.7 (2011 Jul): e1002167.

    Peptidomics of the agriculturally damaging larval stage of the cabbage root fly Delia radicum (Diptera: Anthomyiidae).
    Wegener C
    PloS one 7.7 (2012): e41543.

    Sensorimotor structure of Drosophila larva phototaxis.
    Samuel AD
    Proceedings of the National Academy of Sciences of the United States of America 110.40 (2013 Oct 1): E3868-77.

    Synchronized bilateral synaptic inputs to Drosophila melanogaster neuropeptidergic rest/arousal neurons.
    Nitabach MN
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.22 (2011 Jun 1): 8181-93.

    A role for O-GlcNAcylation in setting circadian clock speed.
    Cho JW
    Genes & development 26.5 (2012 Mar 1): 490-502.

    Drosophila spaghetti and doubletime link the circadian clock and light to caspases, apoptosis and tauopathy.
    Price JL
    PLoS genetics 11.5 (2015 May): e1005171.

    The Drosophila larval visual system: high-resolution analysis of a simple visual neuropil.
    Hartenstein V
    Developmental biology 358.1 (2011 Oct 1): 33-43.

    Using Drosophila as an integrated model to study mild repetitive traumatic brain injury.
    Ratliff EP
    Scientific reports 6. (2016 May 4): 25252.

    Fbxl4 Serves as a Clock Output Molecule that Regulates Sleep through Promotion of Rhythmic Degradation of the GABA<sub>A</sub> Receptor.
    Han J
    Current biology : CB 27.23 (2017 Dec 4): 3616-3625.e5.

    The double-time protein kinase regulates the subcellular localization of the Drosophila clock protein period.
    Blau J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 25.22 (2005 Jun 1): 5430-7.

    The transcription factor Mef2 links the Drosophila core clock to Fas2, neuronal morphology, and circadian behavior.
    Rosbash M
    Neuron 79.2 (2013 Jul 24): 281-92.

    Insulin/IGF-regulated size scaling of neuroendocrine cells expressing the bHLH transcription factor Dimmed in Drosophila.
    Nässel DR
    PLoS genetics 9.12 (2013): e1004052.

    Regulation of circadian behavioral output via a MicroRNA-JAK/STAT circuit.
    Sehgal A
    Cell 148.4 (2012 Feb 17): 765-79.

    Cellular requirements for LARK in the Drosophila circadian system.
    Jackson FR
    Journal of biological rhythms 27.3 (2012 Jun): 183-95.

    Sexual interactions influence the molecular oscillations in DN1 pacemaker neurons in Drosophila melanogaster.
    Yoshii T
    PloS one 8.12 (2013): e84495.

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