SUK 4
Catalog Fields
Clone ID/Product Name: SUK 4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 373178
Clonality: Monoclonal
Immunogen: Partially purified native kinesin from see urchin egg cytosol
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3x635-68.653
Epitope Mapped: Yes
Antigen Name: kinesin heavy chain, head region
Epitope Location or Sequence: Head region of kinesin heavy chain containing the microtubule-binding region. The epitope is located on aa 336-390 of the Drosophila kinesin heavy chain which corresponds to aa 328-382 of the urchin protein.
Alternate Antigen Name:
Deposit Date: 9/12/1991
Antigen Molecular Weight: Predicted: 118 kDa; Apparent: 130 kDa
Depositor: Scholey, J.M.
Antigen Sequence:
Depositor Institution: University of California, Davis
Antigen Species: Sea Urchin
Depositor Notes: Inhibits sea urchin egg kinesin-induced microtubule translocation. SUK 4 doesn't cross react with squid kinesin.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Canine, Drosophila, Human, Mouse, Planaria, Sea Urchin, Shark, Xenopus
Additional Information: In mice, SUK 4 recognizes the three kinesin-1 KIF5A, KIF5B and KIF5C, with a more intense stainning for KIF5A [PMID: 10964943]. In any publications that result from the use of SUK 2, 4, or 5 antibody, Dr. Jonathan Scholey requests that the following be cited: Ingold, A.L., Cohn, S.A., and Scholey, J.M. (1988). Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657-3667.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUK 4 was deposited to the DSHB by Scholey, J.M. (DSHB Hybridoma Product SUK 4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
26 References
Initial Publication
Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
Scholey JM
The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.