SUK 4

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$40.00
SKU: SUK 4
View product citations for antibody SUK 4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: kinesin heavy chain, head region
Hybridoma Cells Available: Yes
Antigen Species: Sea Urchin
Depositor: Scholey, J.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Davis
Positive Tested Species Reactivity: Bovine, Canine, Drosophila, Human, Mouse, Planaria, Sea Urchin, Xenopus
Depositors Notes: Inhibits sea urchin egg kinesin-induced microtubule translocation. SUK 4 doesn't cross react with squid kinesin.
Antigen Molecular Weight: Predicted: 118 kDa; Apparent: 130 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Partially purified native kinesin from see urchin egg cytosol
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x635-68.653
Epitope Location or Sequence: Head region of kinesin heavy chain containing the microtubule-binding region. The epitope is located on aa 336-390 of the Drosophila kinesin heavy chain which corresponds to aa 328-382 of the urchin protein.
Uniprot ID: P35978 
Immunogen Sequence: Full length protein
Entrez Gene ID: 373178 
Additional Characterization:
Antibody Registry ID: AB_528326 
Additional Information: In mice, SUK 4 recognizes the three kinesin-1 KIF5A, KIF5B and KIF5C, with a more intense stainning for KIF5A [PMID: 10964943]. In any publications that result from the use of SUK 2, 4, or 5 antibody, Dr. Jonathan Scholey requests that the following be cited: Ingold, A.L., Cohn, S.A., and Scholey, J.M. (1988). Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657-3667.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SUK 4 was deposited to the DSHB by Scholey, J.M. (DSHB Hybridoma Product SUK 4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

24 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    IF References

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Casein kinase 2 reverses tail-independent inactivation of kinesin-1.
    Gross SP
    Nature communications 3. (2012 Mar 27): 754.

    Spatial and temporal characteristics of normal and perturbed vesicle transport.
    Gunawardena S
    PloS one 9.5 (2014): e97237.

    Drosophila Syd-1, liprin-α, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons.
    Wu C
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.25 (2014 Jun 18): 8474-87.

    Kinesin 5B is necessary for delivery of membrane and receptors during FcγR-mediated phagocytosis.
    Harrison RE
    Journal of immunology (Baltimore, Md. : 1950) 186.2 (2011 Jan 15): 816-25.

    Immunocytochemical localization of synaptic proteins to photoreceptor synapses of Drosophila melanogaster.
    Meinertzhagen IA
    The Journal of comparative neurology 518.7 (2010 Apr 1): 1133-55.

    Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs.
    Elinson RP
    The Journal of cell biology 114.5 (1991 Sep): 1017-28.

    Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles.
    Scholey JM
    Journal of cell science 103 ( Pt 2). (1992 Oct): 309-20.

    Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes.
    Mowry KL
    Developmental cell 15.3 (2008 Sep): 426-36.

    The 55-kDa tumor necrosis factor receptor induces clustering of mitochondria through its membrane-proximal region.
    Grooten J
    The Journal of biological chemistry 273.16 (1998 Apr 17): 9673-80.

    WB References

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    Identification of globular mechanochemical heads of kinesin.
    Goldstein LS
    Nature 338.6213 (1989 Mar 23): 355-7.

    Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.
    Scholey JM
    The Journal of cell biology 113.4 (1991 May): 817-33.

    KIF5C, a novel neuronal kinesin enriched in motor neurons.
    Hirokawa N
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.17 (2000 Sep 1): 6374-84.

    Characterization of axonal transport defects in Drosophila Huntingtin mutants.
    Littleton JT
    Journal of neurogenetics 30.3-4 (2016 Sep - Dec): 212-221.

    Isolation of a sea urchin egg kinesin-related protein using peptide antibodies.
    Scholey JM
    Journal of cell science 101 ( Pt 2). (1992 Feb): 291-301.

    Light chains of sea urchin kinesin identified by immunoadsorption.
    Scholey JM
    Cell motility and the cytoskeleton 16.3 (1990): 204-13.

    Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.
    Wordeman LG
    Journal of cell science 101 ( Pt 2). (1992 Feb): 303-13.

    Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs.
    Elinson RP
    The Journal of cell biology 114.5 (1991 Sep): 1017-28.

    Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles.
    Scholey JM
    Journal of cell science 103 ( Pt 2). (1992 Oct): 309-20.

    The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding.
    Scholey JM
    The Journal of biological chemistry 269.2 (1994 Jan 14): 1477-85.

    The 55-kDa tumor necrosis factor receptor induces clustering of mitochondria through its membrane-proximal region.
    Grooten J
    The Journal of biological chemistry 273.16 (1998 Apr 17): 9673-80.

    IHC References
    IP References

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    Identification of globular mechanochemical heads of kinesin.
    Goldstein LS
    Nature 338.6213 (1989 Mar 23): 355-7.

    Purification and assay of kinesin from sea urchin eggs and early embryos.
    Scholey JM
    Journal of cell science. Supplement 14. (1991): 109-15.

    Casein kinase 2 reverses tail-independent inactivation of kinesin-1.
    Gross SP
    Nature communications 3. (2012 Mar 27): 754.

    Isolation of a sea urchin egg kinesin-related protein using peptide antibodies.
    Scholey JM
    Journal of cell science 101 ( Pt 2). (1992 Feb): 291-301.

    Light chains of sea urchin kinesin identified by immunoadsorption.
    Scholey JM
    Cell motility and the cytoskeleton 16.3 (1990): 204-13.

    Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes.
    Mowry KL
    Developmental cell 15.3 (2008 Sep): 426-36.

    FB References

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    Purification and assay of kinesin from sea urchin eggs and early embryos.
    Scholey JM
    Journal of cell science. Supplement 14. (1991): 109-15.

    A Unique Role for Endothelial Cell Kinesin Light Chain 1, Variant 1 in Leukocyte Transendothelial Migration.
    Muller WA
    The American journal of pathology 186.5 (2016 May): 1375-86.

    Kinesin-1 heavy chain mediates microtubule sliding to drive changes in cell shape.
    Gelfand VI
    Proceedings of the National Academy of Sciences of the United States of America 107.27 (2010 Jul 6): 12151-6.

    Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release.
    Alderton JM
    Science (New York, N.Y.) 263.5145 (1994 Jan 21): 390-3.

    Kinesin- and myosin-driven steps of vesicle recruitment for Ca2+-regulated exocytosis.
    Steinhardt RA
    The Journal of cell biology 138.5 (1997 Sep 8): 999-1008.

    Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes.
    Mowry KL
    Developmental cell 15.3 (2008 Sep): 426-36.

    Epitope Map References

    Identification of globular mechanochemical heads of kinesin.
    Goldstein LS
    Nature 338.6213 (1989 Mar 23): 355-7.

    All References

    Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.
    Scholey JM
    The Journal of cell biology 113.4 (1991 May): 817-33.

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Casein kinase 2 reverses tail-independent inactivation of kinesin-1.
    Gross SP
    Nature communications 3. (2012 Mar 27): 754.

    Spatial and temporal characteristics of normal and perturbed vesicle transport.
    Gunawardena S
    PloS one 9.5 (2014): e97237.

    Drosophila Syd-1, liprin-α, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons.
    Wu C
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.25 (2014 Jun 18): 8474-87.

    Kinesin 5B is necessary for delivery of membrane and receptors during FcγR-mediated phagocytosis.
    Harrison RE
    Journal of immunology (Baltimore, Md. : 1950) 186.2 (2011 Jan 15): 816-25.

    Immunocytochemical localization of synaptic proteins to photoreceptor synapses of Drosophila melanogaster.
    Meinertzhagen IA
    The Journal of comparative neurology 518.7 (2010 Apr 1): 1133-55.

    Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs.
    Elinson RP
    The Journal of cell biology 114.5 (1991 Sep): 1017-28.

    Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles.
    Scholey JM
    Journal of cell science 103 ( Pt 2). (1992 Oct): 309-20.

    Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes.
    Mowry KL
    Developmental cell 15.3 (2008 Sep): 426-36.

    The 55-kDa tumor necrosis factor receptor induces clustering of mitochondria through its membrane-proximal region.
    Grooten J
    The Journal of biological chemistry 273.16 (1998 Apr 17): 9673-80.

    Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    Scholey JM
    The Journal of cell biology 107.6 Pt 2 (1988 Dec): 2657-67.

    Identification of globular mechanochemical heads of kinesin.
    Goldstein LS
    Nature 338.6213 (1989 Mar 23): 355-7.

    KIF5C, a novel neuronal kinesin enriched in motor neurons.
    Hirokawa N
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.17 (2000 Sep 1): 6374-84.

    Characterization of axonal transport defects in Drosophila Huntingtin mutants.
    Littleton JT
    Journal of neurogenetics 30.3-4 (2016 Sep - Dec): 212-221.

    Isolation of a sea urchin egg kinesin-related protein using peptide antibodies.
    Scholey JM
    Journal of cell science 101 ( Pt 2). (1992 Feb): 291-301.

    Light chains of sea urchin kinesin identified by immunoadsorption.
    Scholey JM
    Cell motility and the cytoskeleton 16.3 (1990): 204-13.

    Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.
    Wordeman LG
    Journal of cell science 101 ( Pt 2). (1992 Feb): 303-13.

    The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding.
    Scholey JM
    The Journal of biological chemistry 269.2 (1994 Jan 14): 1477-85.

    Purification and assay of kinesin from sea urchin eggs and early embryos.
    Scholey JM
    Journal of cell science. Supplement 14. (1991): 109-15.

    A Unique Role for Endothelial Cell Kinesin Light Chain 1, Variant 1 in Leukocyte Transendothelial Migration.
    Muller WA
    The American journal of pathology 186.5 (2016 May): 1375-86.

    Kinesin-1 heavy chain mediates microtubule sliding to drive changes in cell shape.
    Gelfand VI
    Proceedings of the National Academy of Sciences of the United States of America 107.27 (2010 Jul 6): 12151-6.

    Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release.
    Alderton JM
    Science (New York, N.Y.) 263.5145 (1994 Jan 21): 390-3.

    Kinesin- and myosin-driven steps of vesicle recruitment for Ca2+-regulated exocytosis.
    Steinhardt RA
    The Journal of cell biology 138.5 (1997 Sep 8): 999-1008.

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