Clone ID / Name: SUK 4
Anti - kinesin heavy chain, head region
Immunofluorescent image of control HeLa cells (top) or KIF5B siRNA transfected HeLa cells (bottom) labeled with DSHB antibody SUK4 against KIF5B (red). Image Credit: J. Nylandsted. PMID: 19242560, 3A
Catalog Fields
Identity
Clone ID / Name: SUK 4
Alternate Antibody Name: —
Antigen Name: kinesin heavy chain, head region
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: —
Entrez Gene ID: 373178
Antigen Sequence: —
Antigen Species: Sea Urchin
Antigen Mol. Weight: Predicted: 118 kDa; Apparent: 130 kDa
Uniprot ID: P35978
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: MIgG1
Myeloma Strain: P3x635-68.653
Antibody Registry / RRID: AB_528326
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Partially purified native kinesin from see urchin egg cytosol
Immunogen Sequence: Full length protein
Epitope Mapped: Yes
Epitope Location / Seq: Head region of kinesin heavy chain containing the microtubule-binding region. The epitope is located on aa 336-390 of the Drosophila kinesin heavy chain which corresponds to aa 328-382 of the urchin protein.
Clone: —
Additional Info: In mice, SUK 4 recognizes the three kinesin-1 KIF5A, KIF5B and KIF5C, with a more intense stainning for KIF5A [PMID: 10964943]. In any publications that result from the use of SUK 2, 4, or 5 antibody, Dr. Jonathan Scholey requests that the following be cited: Ingold, A.L., Cohn, S.A., and Scholey, J.M. (1988). Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657-3667.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Bovine, Canine, Drosophila, Human, Mouse, Planaria, Sea Urchin, Shark, Xenopus
Predicted Reactivity: —
Depositor
Depositor Name: J.M. Scholey
Institution: University of California, Davis
Deposit Date: September 12, 1991
Depositor Notes: Inhibits sea urchin egg kinesin-induced microtubule translocation. SUK 4 doesn't cross react with squid kinesin.
Recommended Applications
Function Blocking
IF
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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