Talin A22A

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$40.00
SKU: Talin A22A
View product citations for antibody Talin A22A on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Talin carboxy terminus 534 amino acids
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: White, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Recommend use as a cocktail of monoclonal antibodies (A22A plus E16B) for immunohistochemistry.
Antigen Molecular Weight: Multiple isoforms; Predicted (isoform B): 307 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: rhea
Immunogen: Recombinant talin, C-terminal (534 amino acids of isoform B)/GST fusion protein
Alternate Gene Names: CG6831, tendrils, Tln, talin
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: C-terminal (aa 2302-2836) of isoform B
Uniprot ID: Q9VSL8 
Immunogen Sequence: Partial protein
Entrez Gene ID: 38978 
Additional Characterization:
Antibody Registry ID: AB_10660289 
Additional Information: This antibody should recognize isoforms B and G and may recognize isoform H, but will not recognize isoforms C or I.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Talin A22A was deposited to the DSHB by White, R. (DSHB Hybridoma Product Talin A22A)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Talin is essential for integrin function in Drosophila.
    Fristrom JW
    Developmental cell 3.4 (2002 Oct): 569-79.

    IF References
    WB References

    Talin is essential for integrin function in Drosophila.
    Fristrom JW
    Developmental cell 3.4 (2002 Oct): 569-79.

    Epitope Map References

    Talin is essential for integrin function in Drosophila.
    Fristrom JW
    Developmental cell 3.4 (2002 Oct): 569-79.

    All References

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