Talin E16B
Catalog Fields
Antigen: Talin carboxy terminus 534 amino acids
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2b
Depositor: White, R.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: University of Cambridge
Antigen Molecular Weight: Multiple isoforms; Predicted (isoform B): 307 kDa
Depositors Notes: Recommend use as a cocktail of monoclonal antibodies (A22A plus E16B) for immunohistochemistry.
Immunogen: Recombinant talin, C-terminal (534 amino acids of isoform B)/GST fusion protein
Gene: rhea
Alternate Antibody Name:
Alternate Gene Names: CG6831, tendrils, Tln, talin
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NSO
Epitope Mapped: Yes
Uniprot ID: Q9VSL8
Epitope Location or Sequence: C-terminal (aa 2302-2836) of isoform B
Entrez Gene ID: 38978
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_10683995
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: This antibody has been suggested to be used in a mix with anti-talin A22A for immunohistochemistry but a number of authors have reported using it alone. This antibody should recognize isoforms B and G and may recognize isoform H, but will not recognize isoforms C or I.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Talin E16B was deposited to the DSHB by White, R. (DSHB Hybridoma Product Talin E16B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Talin is essential for integrin function in Drosophila.
Fristrom JW
Developmental cell 3.4 (2002 Oct): 569-79.