Talin E16B
Catalog Fields
Antigen: Talin carboxy terminus 534 amino acids
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: White, R.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Recommend use as a cocktail of monoclonal antibodies (A22A plus E16B) for immunohistochemistry.
Predicted Species Reactivity:
Gene: rhea
Immunogen: Recombinant talin, C-terminal (534 amino acids of isoform B)/GST fusion protein
Alternate Gene Names: CG6831, tendrils, Tln, talin
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: C-terminal (aa 2302-2836) of isoform B
Uniprot ID: Q9VSL8
Immunogen Sequence: Partial protein
Entrez Gene ID: 38978
Antibody Registry ID: AB_10683995
Additional Information: This antibody has been suggested to be used in a mix with anti-talin A22A for immunohistochemistry but a number of authors have reported using it alone. This antibody should recognize isoforms B and G and may recognize isoform H, but will not recognize isoforms C or I.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Talin E16B was deposited to the DSHB by White, R. (DSHB Hybridoma Product Talin E16B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Talin is essential for integrin function in Drosophila.
Fristrom JW
Developmental cell 3.4 (2002 Oct): 569-79.