DK.1A4

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SKU: DK.1A4

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DSHB Data Sheet

Catalog Fields

Product Name/ID: DK.1A4
Available to For-Profits: Yes
Alternate Antibody Name: PS1, alphaPS1
Gene Name: mew
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528303 
Uniprot ID: Q24247 
RRID:  
Entrez Gene ID: 32275 
Clonality: Monoclonal
Immunogen: Sonicated imaginal discs
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: P3/NSI
Epitope Mapped: No
Antigen Name: integrin alphaPS1 (multiple edematous wing)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/17/2002
Antigen Molecular Weight: Multiple isoforms
Depositor: Brower, D.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Drosophila
Depositor Notes: This antibody doesn't work for immunoblotting and is aldehyde sensitive.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Integrins are heterodimers of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain (resulting from the cleavage of the mew gene product for alphaPS1) linked by a disulfide bond. AlphaPS1 associates with betaPS.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DK.1A4 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product DK.1A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

12 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    A position-specific cell surface antigen in the drosophila wing imaginal disc.
    Smith RJ
    Cell 25.1 (1981 Jul): 159-64.

    Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs.
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    The integrin adhesion complex changes its composition and function during morphogenesis of an epithelium.
    Brown NH
    Journal of cell science 122.Pt 23 (2009 Dec 1): 4363-74.

    Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression.
    Salzberg A
    Developmental biology 351.1 (2011 Mar 1): 99-109.

    Integrins modulate Sog activity in the Drosophila wing.
    Bier E
    Development (Cambridge, England) 130.16 (2003 Aug): 3851-64.

    The role of apterous in the control of dorsoventral compartmentalization and PS integrin gene expression in the developing wing of Drosophila.
    Zavortink M
    Development (Cambridge, England) 120.7 (1994 Jul): 1805-15.

    Integrin alpha chains exhibit distinct temporal and spatial localization patterns in epithelial cells of the Drosophila ovary.
    Lemosy EK
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3927-39.

    Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons.
    Grueber WB
    Neuron 73.1 (2012 Jan 12): 79-91.

    Genetic interaction between integrins and moleskin, a gene encoding a Drosophila homolog of importin-7.
    Brower DL
    Genetics 162.1 (2002 Sep): 285-96.

    Developmental analysis of Drosophila position-specific antigens.
    Reger LA
    Developmental biology 108.1 (1985 Mar): 120-30.

    IHC References

    Genetic analysis on the role of integrin during axon guidance in Drosophila.
    Chiba A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.19 (1998 Oct 1): 7847-55.

    IP References

    Differentiation, extracellular matrix synthesis, and integrin assembly by Drosophila embryo cells cultured on vitronectin and laminin substrates.
    Fessler JH
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 116-28.

    Integrins modulate Sog activity in the Drosophila wing.
    Bier E
    Development (Cambridge, England) 130.16 (2003 Aug): 3851-64.

    All References

    Genetic analysis on the role of integrin during axon guidance in Drosophila.
    Chiba A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.19 (1998 Oct 1): 7847-55.

    A position-specific cell surface antigen in the drosophila wing imaginal disc.
    Smith RJ
    Cell 25.1 (1981 Jul): 159-64.

    Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs.
    Reger LA
    Proceedings of the National Academy of Sciences of the United States of America 81.23 (1984 Dec): 7485-9.

    The integrin adhesion complex changes its composition and function during morphogenesis of an epithelium.
    Brown NH
    Journal of cell science 122.Pt 23 (2009 Dec 1): 4363-74.

    Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression.
    Salzberg A
    Developmental biology 351.1 (2011 Mar 1): 99-109.

    Integrins modulate Sog activity in the Drosophila wing.
    Bier E
    Development (Cambridge, England) 130.16 (2003 Aug): 3851-64.

    The role of apterous in the control of dorsoventral compartmentalization and PS integrin gene expression in the developing wing of Drosophila.
    Zavortink M
    Development (Cambridge, England) 120.7 (1994 Jul): 1805-15.

    Integrin alpha chains exhibit distinct temporal and spatial localization patterns in epithelial cells of the Drosophila ovary.
    Lemosy EK
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3927-39.

    Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons.
    Grueber WB
    Neuron 73.1 (2012 Jan 12): 79-91.

    Genetic interaction between integrins and moleskin, a gene encoding a Drosophila homolog of importin-7.
    Brower DL
    Genetics 162.1 (2002 Sep): 285-96.

    Developmental analysis of Drosophila position-specific antigens.
    Reger LA
    Developmental biology 108.1 (1985 Mar): 120-30.

    Differentiation, extracellular matrix synthesis, and integrin assembly by Drosophila embryo cells cultured on vitronectin and laminin substrates.
    Fessler JH
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 116-28.

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