DK.1A4
Catalog Fields
Antigen: integrin alphaPS1 (multiple edematous wing)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Brower, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody doesn't work for immunoblotting and is aldehyde sensitive.
Predicted Species Reactivity:
Gene: mew
Immunogen: Sonicated imaginal discs
Alternate Gene Names: alphaPS1, CG1771
Alternate Antibody Name: PS1, alphaPS1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3/NSI
Epitope Location or Sequence:
Uniprot ID: Q24247
Immunogen Sequence: Total protein
Entrez Gene ID: 32275
Antibody Registry ID: AB_528303
Additional Information: Integrins are heterodimers of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain (resulting from the cleavage of the mew gene product for alphaPS1) linked by a disulfide bond. AlphaPS1 associates with betaPS.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DK.1A4 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product DK.1A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
A position-specific cell surface antigen in the drosophila wing imaginal disc.
Smith RJ
Cell 25.1 (1981 Jul): 159-64.