DK.1A4
Catalog Fields
Antigen: integrin alphaPS1 (multiple edematous wing)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Brower, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody doesn't work for immunoblotting and is aldehyde sensitive.
Antigen Molecular Weight: Multiple isoforms
Human Protein Atlas:
Predicted Species Reactivity:
Gene: mew
Immunogen: Sonicated imaginal discs
Alternate Gene Names: alphaPS1, CG1771
Alternate Antibody Name: PS1, alphaPS1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3/NSI
Epitope Location or Sequence:
Uniprot ID: Q24247
Immunogen Sequence: Total protein
Entrez Gene ID: 32275
Additional Characterization:
Antibody Registry ID: AB_528303
Additional Information: Integrins are heterodimers of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain (resulting from the cleavage of the mew gene product for alphaPS1) linked by a disulfide bond. AlphaPS1 associates with betaPS.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DK.1A4 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product DK.1A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
A position-specific cell surface antigen in the drosophila wing imaginal disc.
Smith RJ
Cell 25.1 (1981 Jul): 159-64.