anti-Antp 8C11

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SKU: anti-Antp 8C11
View product citations for antibody anti-Antp 8C11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Antennapedia protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Brower, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Arizona
Positive Tested Species Reactivity: Drosophila, Grasshopper
Depositors Notes: Species Specificity: Drosophila
Antigen Molecular Weight: Predicted: 43kDa (multiple isoforms from 33 to 43kDa)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Antp
Immunogen: lacZ-Antp fusion protein
Alternate Gene Names: AntP; ANTP; AntP1;Dmel\CG1028;
Alternate Antibody Name: 8C11-1; 8C11.1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NSO
Epitope Location or Sequence:
Uniprot ID: P02833 
Immunogen Sequence:
Entrez Gene ID: 40835 
Additional Characterization:
Antibody Registry ID: AB_528083 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Antp 8C11 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product anti-Antp 8C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

28 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References
    IHC References
    All References

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