anti-Antp 8C11
Catalog Fields
Antigen: Antennapedia protein
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Brower, D.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila, Grasshopper
Depositors Institution: University of Arizona
Antigen Molecular Weight: Predicted: 43kDa (multiple isoforms from 33 to 43kDa)
Depositors Notes: Species Specificity: Drosophila
Immunogen: lacZ-Antp fusion protein
Gene: Antp
Alternate Antibody Name: 8C11-1; 8C11.1
Alternate Gene Names: AntP; ANTP; AntP1;Dmel\CG1028;
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NSO
Epitope Mapped: No
Uniprot ID: P02833
Epitope Location or Sequence:
Entrez Gene ID: 40835
Immunogen Sequence:
Antibody Registry ID: AB_528083
Recommended Applications: Immunofluorescence, Immunohistochemistry
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Antp 8C11 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product anti-Antp 8C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
30 References
Initial Publication
The involvement of lysophosphoglycerides in neurotransmitter release; the composition and turnover of phospholipids of synaptic vesicles of guinea-pig cerebral cortex and Torpedo electric organ and the effect of stimulation.
Whittaker VP
Brain research 100.3 (1975 Dec 26): 629-44.