anti-Antp 8C11

(0)No Reviews yet
$45.00 to $425.00
SKU: anti-Antp 8C11
View product citations for antibody anti-Antp 8C11 on CiteAb

In Stock

Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: Antennapedia protein
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Brower, D.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila, Grasshopper
Depositors Institution: University of Arizona
Antigen Molecular Weight: Predicted: 43kDa (multiple isoforms from 33 to 43kDa)
Depositors Notes: Species Specificity: Drosophila
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: lacZ-Antp fusion protein
Gene: Antp
Alternate Antibody Name: 8C11-1; 8C11.1
Alternate Gene Names: AntP; ANTP; AntP1;Dmel\CG1028;
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NSO
Epitope Mapped: No
Uniprot ID: P02833 
Epitope Location or Sequence:
Entrez Gene ID: 40835 
Immunogen Sequence:
Antibody Registry ID: AB_528083 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Antp 8C11 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product anti-Antp 8C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

30 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway.
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    Chromatin remodeling protein INO80 has a role in regulation of homeotic gene expression in Drosophila.
    Brahmachari V
    Genes to cells : devoted to molecular & cellular mechanisms 15.7 (2010 Jun): 725-35.

    Dual role for Hox genes and Hox co-factors in conferring leg motoneuron survival and identity in Drosophila.
    Mann RS
    Development (Cambridge, England) 140.9 (2013 May): 2027-38.

    Cross-regulatory protein-protein interactions between Hox and Pax transcription factors.
    Gehring WJ
    Proceedings of the National Academy of Sciences of the United States of America 105.36 (2008 Sep 9): 13439-44.

    Sumoylation is tumor-suppressive and confers proliferative quiescence to hematopoietic progenitors in Drosophila melanogaster larvae.
    Govind S
    Biology open 1.3 (2012 Mar 15): 161-72.

    Polydnaviral ankyrin proteins aid parasitic wasp survival by coordinate and selective inhibition of hematopoietic and immune NF-kappa B signaling in insect hosts.
    Govind S
    PLoS pathogens 9.8 (2013): e1003580.

    Retromer promotes immune quiescence by suppressing Spätzle-Toll pathway in Drosophila.
    Lin X
    Journal of cellular physiology 229.4 (2014 Apr): 512-520.

    The YPWM motif links Antennapedia to the basal transcriptional machinery.
    Gehring WJ
    Development (Cambridge, England) 135.9 (2008 May): 1669-79.

    Cell-type-specific mechanisms of transcriptional repression by the homeotic gene products UBX and ABD-A in Drosophila embryos.
    Sakonju S
    The EMBO journal 12.3 (1993 Mar): 1099-109.

    Control of antennal versus leg development in Drosophila.
    Mann RS
    Nature 392.6677 (1998 Apr 16): 723-6.

    Misregulation of homeotic gene expression in Drosophila larvae resulting from mutations at the extra sex combs locus.
    Brower DL
    Developmental biology 126.2 (1988 Apr): 219-27.

    Homeotic Complex (Hox) gene regulation and homeosis in the mesoderm of the Drosophila melanogaster embryo: the roles of signal transduction and cell autonomous regulation.
    Kaufman TC
    Mechanisms of development 102.1-2 (2001 Apr): 17-32.

    Drosophila cardiac tube organogenesis requires multiple phases of Hox activity.
    Semeriva M
    Developmental biology 272.2 (2004 Aug 15): 419-31.

    Persistent ectopic expression of Drosophila homeotic genes resulting from maternal deficiency of the extra sex combs gene product.
    Brower DL
    Developmental biology 142.2 (1990 Dec): 422-31.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit in vivo reprogramming of the developing Drosophila eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila.
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

    Hox dosage contributes to flight appendage morphology in Drosophila.
    Merabet S
    Nature communications 12.1 (2021 May 17): 2892.

    IHC References

    Sequence and expression of grasshopper antennapedia: comparison to Drosophila.
    Ball EE
    Developmental biology 172.2 (1995 Dec): 452-65.

    The Drosophila homeotic target gene centrosomin (cnn) encodes a novel centrosomal protein with leucine zippers and maps to a genomic region required for midgut morphogenesis.
    Kaufman TC
    Development (Cambridge, England) 121.11 (1995 Nov): 3861-76.

    Misregulation of homeotic gene expression in Drosophila larvae resulting from mutations at the extra sex combs locus.
    Brower DL
    Developmental biology 126.2 (1988 Apr): 219-27.

    Expression of the beta3 tubulin gene (beta Tub60D) in the visceral mesoderm of Drosophila is dependent on a complex enhancer that binds Tinman and UBX.
    Renkawitz-Pohl R
    Molecular & general genetics : MGG 262.4-5 (1999 Dec): 643-58.

    Homeotic Complex (Hox) gene regulation and homeosis in the mesoderm of the Drosophila melanogaster embryo: the roles of signal transduction and cell autonomous regulation.
    Kaufman TC
    Mechanisms of development 102.1-2 (2001 Apr): 17-32.

    Persistent ectopic expression of Drosophila homeotic genes resulting from maternal deficiency of the extra sex combs gene product.
    Brower DL
    Developmental biology 142.2 (1990 Dec): 422-31.

    SETDB1 modulates the differentiation of both the crystal cells and the lamellocytes in Drosophila.
    Mishra RK
    Developmental biology 456.1 (2019 Dec 1): 74-85.

    Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland.
    Mandal L
    PLoS genetics 17.8 (2021 Aug): e1009709.

    All References

    Sequence and expression of grasshopper antennapedia: comparison to Drosophila.
    Ball EE
    Developmental biology 172.2 (1995 Dec): 452-65.

    The Drosophila homeotic target gene centrosomin (cnn) encodes a novel centrosomal protein with leucine zippers and maps to a genomic region required for midgut morphogenesis.
    Kaufman TC
    Development (Cambridge, England) 121.11 (1995 Nov): 3861-76.

    Misregulation of homeotic gene expression in Drosophila larvae resulting from mutations at the extra sex combs locus.
    Brower DL
    Developmental biology 126.2 (1988 Apr): 219-27.

    Expression of the beta3 tubulin gene (beta Tub60D) in the visceral mesoderm of Drosophila is dependent on a complex enhancer that binds Tinman and UBX.
    Renkawitz-Pohl R
    Molecular & general genetics : MGG 262.4-5 (1999 Dec): 643-58.

    Homeotic Complex (Hox) gene regulation and homeosis in the mesoderm of the Drosophila melanogaster embryo: the roles of signal transduction and cell autonomous regulation.
    Kaufman TC
    Mechanisms of development 102.1-2 (2001 Apr): 17-32.

    Persistent ectopic expression of Drosophila homeotic genes resulting from maternal deficiency of the extra sex combs gene product.
    Brower DL
    Developmental biology 142.2 (1990 Dec): 422-31.

    SETDB1 modulates the differentiation of both the crystal cells and the lamellocytes in Drosophila.
    Mishra RK
    Developmental biology 456.1 (2019 Dec 1): 74-85.

    Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland.
    Mandal L
    PLoS genetics 17.8 (2021 Aug): e1009709.

    Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway.
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    Chromatin remodeling protein INO80 has a role in regulation of homeotic gene expression in Drosophila.
    Brahmachari V
    Genes to cells : devoted to molecular & cellular mechanisms 15.7 (2010 Jun): 725-35.

    Dual role for Hox genes and Hox co-factors in conferring leg motoneuron survival and identity in Drosophila.
    Mann RS
    Development (Cambridge, England) 140.9 (2013 May): 2027-38.

    Cross-regulatory protein-protein interactions between Hox and Pax transcription factors.
    Gehring WJ
    Proceedings of the National Academy of Sciences of the United States of America 105.36 (2008 Sep 9): 13439-44.

    Sumoylation is tumor-suppressive and confers proliferative quiescence to hematopoietic progenitors in Drosophila melanogaster larvae.
    Govind S
    Biology open 1.3 (2012 Mar 15): 161-72.

    Polydnaviral ankyrin proteins aid parasitic wasp survival by coordinate and selective inhibition of hematopoietic and immune NF-kappa B signaling in insect hosts.
    Govind S
    PLoS pathogens 9.8 (2013): e1003580.

    Retromer promotes immune quiescence by suppressing Spätzle-Toll pathway in Drosophila.
    Lin X
    Journal of cellular physiology 229.4 (2014 Apr): 512-520.

    The YPWM motif links Antennapedia to the basal transcriptional machinery.
    Gehring WJ
    Development (Cambridge, England) 135.9 (2008 May): 1669-79.

    Cell-type-specific mechanisms of transcriptional repression by the homeotic gene products UBX and ABD-A in Drosophila embryos.
    Sakonju S
    The EMBO journal 12.3 (1993 Mar): 1099-109.

    Control of antennal versus leg development in Drosophila.
    Mann RS
    Nature 392.6677 (1998 Apr 16): 723-6.

    Drosophila cardiac tube organogenesis requires multiple phases of Hox activity.
    Semeriva M
    Developmental biology 272.2 (2004 Aug 15): 419-31.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit in vivo reprogramming of the developing Drosophila eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila.
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

    Hox dosage contributes to flight appendage morphology in Drosophila.
    Merabet S
    Nature communications 12.1 (2021 May 17): 2892.

    The involvement of lysophosphoglycerides in neurotransmitter release; the composition and turnover of phospholipids of synaptic vesicles of guinea-pig cerebral cortex and Torpedo electric organ and the effect of stimulation.
    Whittaker VP
    Brain research 100.3 (1975 Dec 26): 629-44.

    Steroid hormone-dependent transformation of polyhomeotic mutant neurons in the Drosophila brain.
    Lee T
    Development (Cambridge, England) 133.7 (2006 Apr): 1231-40.

    Mis-specified cells die by an active gene-directed process, and inhibition of this death results in cell fate transformation in Drosophila.
    Bergmann A
    Development (Cambridge, England) 132.24 (2005 Dec): 5343-52.

    Polycomb genes interact with the tumor suppressor genes hippo and warts in the maintenance of Drosophila sensory neuron dendrites.
    Jan YN
    Genes & development 21.8 (2007 Apr 15): 956-72.

    Cross-regulation of Hox genes in the Drosophila melanogaster embryo.
    Kaufman T
    Mechanisms of development 102.1-2 (2001 Apr): 3-16.

    Coordinate regulation of downstream genes by extradenticle and the homeotic selector proteins.
    Wieschaus E
    The EMBO journal 13.15 (1994 Aug 1): 3561-9.

    Homeotic genes have specific functional roles in the establishment of the Drosophila embryonic peripheral nervous system.
    Kaufman TC
    Development (Cambridge, England) 115.1 (1992 May): 35-47.

    Requirements for transcriptional repression and activation by Engrailed in Drosophila embryos.
    Vincent JP
    Development (Cambridge, England) 130.4 (2003 Feb): 729-39.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review