Antigen: Antennapedia protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Brower, D.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: University of Arizona
Positive Tested Species Reactivity: Drosophila, Grasshopper
Depositors Notes: Species Specificity: Drosophila
Antigen Molecular Weight: Predicted: 43kDa (multiple isoforms from 33 to 43kDa)
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: lacZ-Antp fusion protein
Alternate Gene Names: AntP; ANTP; AntP1;Dmel\CG1028;
Alternate Antibody Name: 8C11-1; 8C11.1
Alternate Antigen Name:
Myeloma Strain: NSO
Epitope Location or Sequence:
Uniprot ID: P02833
Entrez Gene ID: 40835
Antibody Registry ID: AB_528083
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Antp 8C11 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product anti-Antp 8C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).