anti-Antp 4C3

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$40.00
SKU: anti-Antp 4C3
View product citations for antibody anti-Antp 4C3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Antennapedia protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Brower, D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Arizona
Positive Tested Species Reactivity: Beetle, Broad species, Drosophila, Grasshopper
Depositors Notes: Species Specificity: shows broad species binding.
Antigen Molecular Weight: Predicted: 43kDa (multiple isoforms from 33 to 43kDa)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Antp
Immunogen: lacZ-Antp fusion protein
Alternate Gene Names: AntP; ANTP; AntP1;Dmel\CG1028;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NSO
Epitope Location or Sequence:
Uniprot ID: P02833 
Immunogen Sequence:
Entrez Gene ID: 40835 
Additional Characterization:
Antibody Registry ID: AB_528082 
Additional Information:
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Antp 4C3 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product anti-Antp 4C3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

32 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control.
    Saurin AJ
    EMBO reports 9.3 (2008 Mar): 260-6.

    Ebi alleviates excessive growth signaling through multiple epigenetic functions in Drosophila.
    Tsuda L
    Genes to cells : devoted to molecular and cellular mechanisms 18.11 (2013 Nov): 909-20.

    The homeobox gene cut interacts genetically with the homeotic genes proboscipedia and Antennapedia.
    Blochlinger K
    Genetics 149.1 (1998 May): 131-42.

    Serpent, suppressor of hairless and U-shaped are crucial regulators of hedgehog niche expression and prohemocyte maintenance during Drosophila larval hematopoiesis.
    Schulz RA
    Development (Cambridge, England) 137.21 (2010 Nov): 3561-8.

    bantam miRNA is important for Drosophila blood cell homeostasis and a regulator of proliferation in the hematopoietic progenitor niche.
    Schulz RA
    Biochemical and biophysical research communications 453.3 (2014 Oct 24): 467-72.

    Asrij maintains the stem cell niche and controls differentiation during Drosophila lymph gland hematopoiesis.
    Inamdar MS
    PloS one 6.11 (2011): e27667.

    A unique Extradenticle recruitment mode in the Drosophila Hox protein Ultrabithorax.
    Graba Y
    Proceedings of the National Academy of Sciences of the United States of America 104.43 (2007 Oct 23): 16946-51.

    Segment-specific prevention of pioneer neuron apoptosis by cell-autonomous, postmitotic Hox gene activity.
    Thor S
    Development (Cambridge, England) 131.24 (2004 Dec): 6093-105.

    Gene regulatory networks controlling hematopoietic progenitor niche cell production and differentiation in the Drosophila lymph gland.
    Schulz RA
    PloS one 7.7 (2012): e41604.

    Additional sex combs affects antennal development by means of spatially restricted repression of Antp and wg.
    Salzberg A
    Developmental dynamics : an official publication of the American Association of Anatomists 236.8 (2007 Aug): 2118-30.

    A single origin for nymphalid butterfly eyespots followed by widespread loss of associated gene expression.
    Monteiro A
    PLoS genetics 8.8 (2012): e1002893.

    Cross-regulatory protein-protein interactions between Hox and Pax transcription factors.
    Gehring WJ
    Proceedings of the National Academy of Sciences of the United States of America 105.36 (2008 Sep 9): 13439-44.

    Over-expression of Ultrabithorax alters embryonic body plan and wing patterns in the butterfly Bicyclus anynana.
    Monteiro A
    Developmental biology 394.2 (2014 Oct 15): 357-66.

    Insights into Hox protein function from a large scale combinatorial analysis of protein domains.
    Graba Y
    PLoS genetics 7.10 (2011 Oct): e1002302.

    Three Drosophila Hox complex microRNAs do not have major effects on expression of evolutionarily conserved Hox gene targets during embryogenesis.
    McGinnis W
    PloS one 7.2 (2012): e31365.

    The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system.
    Alonso CR
    Development (Cambridge, England) 141.10 (2014 May): 2046-56.

    Misregulation of homeotic gene expression in Drosophila larvae resulting from mutations at the extra sex combs locus.
    Brower DL
    Developmental biology 126.2 (1988 Apr): 219-27.

    Persistent ectopic expression of Drosophila homeotic genes resulting from maternal deficiency of the extra sex combs gene product.
    Brower DL
    Developmental biology 142.2 (1990 Dec): 422-31.

    WB References

    The YPWM motif links Antennapedia to the basal transcriptional machinery.
    Gehring WJ
    Development (Cambridge, England) 135.9 (2008 May): 1669-79.

    IHC References

    Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control.
    Saurin AJ
    EMBO reports 9.3 (2008 Mar): 260-6.

    Drosophila midgut morphogenesis requires the function of the segmentation gene odd-paired.
    Sakonju S
    Developmental biology 169.2 (1995 Jun): 580-95.

    The Tribolium homeotic gene Abdominal is homologous to abdominal-A of the Drosophila bithorax complex.
    Denell RE
    Development (Cambridge, England) 117.1 (1993 Jan): 233-43.

    Sequence and expression of grasshopper antennapedia: comparison to Drosophila.
    Ball EE
    Developmental biology 172.2 (1995 Dec): 452-65.

    Misregulation of homeotic gene expression in Drosophila larvae resulting from mutations at the extra sex combs locus.
    Brower DL
    Developmental biology 126.2 (1988 Apr): 219-27.

    Expression and function of the homoeotic genes Antennapedia and Sex combs reduced in the embryonic midgut of Drosophila.
    Scott MP
    Development (Cambridge, England) 109.2 (1990 Jun): 289-303.

    Trithorax regulates multiple homeotic genes in the bithorax and Antennapedia complexes and exerts different tissue-specific, parasegment-specific and promoter-specific effects on each.
    Harte PJ
    Development (Cambridge, England) 117.1 (1993 Jan): 119-34.

    Persistent ectopic expression of Drosophila homeotic genes resulting from maternal deficiency of the extra sex combs gene product.
    Brower DL
    Developmental biology 142.2 (1990 Dec): 422-31.

    Ectopic expression and function of the Antp and Scr homeotic genes: the N terminus of the homeodomain is critical to functional specificity.
    Scott MP
    Development (Cambridge, England) 118.2 (1993 Jun): 339-52.

    Gel Supershift References
    All References

    Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control.
    Saurin AJ
    EMBO reports 9.3 (2008 Mar): 260-6.

    Drosophila midgut morphogenesis requires the function of the segmentation gene odd-paired.
    Sakonju S
    Developmental biology 169.2 (1995 Jun): 580-95.

    The Tribolium homeotic gene Abdominal is homologous to abdominal-A of the Drosophila bithorax complex.
    Denell RE
    Development (Cambridge, England) 117.1 (1993 Jan): 233-43.

    Sequence and expression of grasshopper antennapedia: comparison to Drosophila.
    Ball EE
    Developmental biology 172.2 (1995 Dec): 452-65.

    Misregulation of homeotic gene expression in Drosophila larvae resulting from mutations at the extra sex combs locus.
    Brower DL
    Developmental biology 126.2 (1988 Apr): 219-27.

    Expression and function of the homoeotic genes Antennapedia and Sex combs reduced in the embryonic midgut of Drosophila.
    Scott MP
    Development (Cambridge, England) 109.2 (1990 Jun): 289-303.

    Trithorax regulates multiple homeotic genes in the bithorax and Antennapedia complexes and exerts different tissue-specific, parasegment-specific and promoter-specific effects on each.
    Harte PJ
    Development (Cambridge, England) 117.1 (1993 Jan): 119-34.

    Persistent ectopic expression of Drosophila homeotic genes resulting from maternal deficiency of the extra sex combs gene product.
    Brower DL
    Developmental biology 142.2 (1990 Dec): 422-31.

    Ectopic expression and function of the Antp and Scr homeotic genes: the N terminus of the homeodomain is critical to functional specificity.
    Scott MP
    Development (Cambridge, England) 118.2 (1993 Jun): 339-52.

    Ebi alleviates excessive growth signaling through multiple epigenetic functions in Drosophila.
    Tsuda L
    Genes to cells : devoted to molecular and cellular mechanisms 18.11 (2013 Nov): 909-20.

    The homeobox gene cut interacts genetically with the homeotic genes proboscipedia and Antennapedia.
    Blochlinger K
    Genetics 149.1 (1998 May): 131-42.

    Serpent, suppressor of hairless and U-shaped are crucial regulators of hedgehog niche expression and prohemocyte maintenance during Drosophila larval hematopoiesis.
    Schulz RA
    Development (Cambridge, England) 137.21 (2010 Nov): 3561-8.

    bantam miRNA is important for Drosophila blood cell homeostasis and a regulator of proliferation in the hematopoietic progenitor niche.
    Schulz RA
    Biochemical and biophysical research communications 453.3 (2014 Oct 24): 467-72.

    Asrij maintains the stem cell niche and controls differentiation during Drosophila lymph gland hematopoiesis.
    Inamdar MS
    PloS one 6.11 (2011): e27667.

    A unique Extradenticle recruitment mode in the Drosophila Hox protein Ultrabithorax.
    Graba Y
    Proceedings of the National Academy of Sciences of the United States of America 104.43 (2007 Oct 23): 16946-51.

    Segment-specific prevention of pioneer neuron apoptosis by cell-autonomous, postmitotic Hox gene activity.
    Thor S
    Development (Cambridge, England) 131.24 (2004 Dec): 6093-105.

    Gene regulatory networks controlling hematopoietic progenitor niche cell production and differentiation in the Drosophila lymph gland.
    Schulz RA
    PloS one 7.7 (2012): e41604.

    Additional sex combs affects antennal development by means of spatially restricted repression of Antp and wg.
    Salzberg A
    Developmental dynamics : an official publication of the American Association of Anatomists 236.8 (2007 Aug): 2118-30.

    A single origin for nymphalid butterfly eyespots followed by widespread loss of associated gene expression.
    Monteiro A
    PLoS genetics 8.8 (2012): e1002893.

    Cross-regulatory protein-protein interactions between Hox and Pax transcription factors.
    Gehring WJ
    Proceedings of the National Academy of Sciences of the United States of America 105.36 (2008 Sep 9): 13439-44.

    Over-expression of Ultrabithorax alters embryonic body plan and wing patterns in the butterfly Bicyclus anynana.
    Monteiro A
    Developmental biology 394.2 (2014 Oct 15): 357-66.

    Insights into Hox protein function from a large scale combinatorial analysis of protein domains.
    Graba Y
    PLoS genetics 7.10 (2011 Oct): e1002302.

    Three Drosophila Hox complex microRNAs do not have major effects on expression of evolutionarily conserved Hox gene targets during embryogenesis.
    McGinnis W
    PloS one 7.2 (2012): e31365.

    The RNA-binding protein ELAV regulates Hox RNA processing, expression and function within the Drosophila nervous system.
    Alonso CR
    Development (Cambridge, England) 141.10 (2014 May): 2046-56.

    The YPWM motif links Antennapedia to the basal transcriptional machinery.
    Gehring WJ
    Development (Cambridge, England) 135.9 (2008 May): 1669-79.

    [Demonstration of tumor inhibiting properties of a strongly immunostimulating low-molecular weight substance. Comparative studies with ifosfamide on the immuno-labile DS carcinosarcoma. Stimulation of the autoimmune activity for approx. 20 days by BA 1, a N-(2-cyanoethylene)-urea. Novel prophylactic possibilities].
    Reitnauer PG
    Arzneimittel-Forschung 25.9 (1975 Sep): 1369-79.

    Hox transcription factor Antp regulates sericin-1 gene expression in the terminal differentiated silk gland of Bombyx mori.
    Takiya S
    Developmental biology 386.1 (2014 Feb 1): 64-71.

    Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.
    Gould AP
    PLoS biology 6.3 (2008 Mar 11): e58.

    Drosophila retinal pigment cell death is regulated in a position-dependent manner by a cell memory gene.
    Théodore L
    The International journal of developmental biology 52.1 (2008): 21-31.

    Polycomb genes interact with the tumor suppressor genes hippo and warts in the maintenance of Drosophila sensory neuron dendrites.
    Jan YN
    Genes and development 21.8 (2007 Apr 15): 956-72.

    Evolving role of Antennapedia protein in arthropod limb patterning.
    Hayashi S
    Development (Cambridge, England) 129.15 (2002 Aug): 3555-61.

    Homeotic genes regulate the spatial expression of putative growth factors in the visceral mesoderm of Drosophila embryos.
    Scott MP
    Development (Cambridge, England) 110.4 (1990 Dec): 1031-40.

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