Antigen: Sex combs reduced homeotic protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Brower, D.
Host Species: mouse
Depositors Institution: University of Arizona
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The depositor indicates this antibody works for immunprecipitation applications.
Antigen Molecular Weight: Predicted: 44-kDa; Appparent: 54-58kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant LacZ-Scr Protein fragment
Alternate Gene Names: CG1030; Dmel\CG1030; DmScr; SCR
Alternate Antibody Name: anti-Scr
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: a.a. 80-131 (including a.a. 114-117)
Uniprot ID: P09077
Immunogen Sequence: a. a. 80-417
Entrez Gene ID: 40833
Antibody Registry ID: AB_528462
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Scr 6H4.1 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product anti-Scr 6H4.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References