anti-Scr 6H4.1

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SKU: anti-Scr 6H4.1
View product citations for antibody anti-Scr 6H4.1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Sex combs reduced homeotic protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Brower, D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Arizona
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The depositor indicates this antibody works for immunprecipitation applications.
Antigen Molecular Weight: Predicted: 44-kDa; Appparent: 54-58kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Scr
Immunogen: Recombinant LacZ-Scr Protein fragment
Alternate Gene Names: CG1030; Dmel\CG1030; DmScr; SCR
Alternate Antibody Name: anti-Scr
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: a.a. 80-131 (including a.a. 114-117)
Uniprot ID: P09077 
Immunogen Sequence: a. a. 80-417
Entrez Gene ID: 40833 
Additional Characterization:  
Antibody Registry ID: AB_528462 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Scr 6H4.1 was deposited to the DSHB by Brower, D. (DSHB Hybridoma Product anti-Scr 6H4.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Expression of the Sex combs reduced protein in Drosophila larvae.
    Brower DL
    Developmental biology 127.1 (1988 May): 113-8.

    IF References
    WB References
    IHC References
    Epitope Map References
    All References

    Male- and female-specific variants of doublesex gene products have different roles to play towards regulation of Sex combs reduced expression and sex comb morphogenesis in Drosophila.
    Shyamala BV
    Journal of biosciences 38.3 (2013 Sep): 455-60.

    Evolution of sex comb from the primitive bristle pattern in Drosophila is associated with modification in the developmental regulatory protein Dachshund.
    Shyamala BV
    Genesis (New York, N.Y. : 2000) 51.2 (2013 Feb): 97-109.

    Drosophila Reptin and other TIP60 complex components promote generation of silent chromatin.
    Mannervik M
    Genetics 174.1 (2006 Sep): 241-51.

    Sex-specific expression of a HOX gene associated with rapid morphological evolution.
    Kopp A
    Developmental biology 311.2 (2007 Nov 15): 277-86.

    Expression of the Sex combs reduced protein in Drosophila larvae.
    Brower DL
    Developmental biology 127.1 (1988 May): 113-8.

    Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control.
    Saurin AJ
    EMBO reports 9.3 (2008 Mar): 260-6.

    Chromatin remodeling protein INO80 has a role in regulation of homeotic gene expression in Drosophila.
    Brahmachari V
    Genes to cells : devoted to molecular & cellular mechanisms 15.7 (2010 Jun): 725-35.

    Evolution of sex-specific traits through changes in HOX-dependent doublesex expression.
    Kopp A
    PLoS biology 9.8 (2011 Aug): e1001131.

    Three Drosophila Hox complex microRNAs do not have major effects on expression of evolutionarily conserved Hox gene targets during embryogenesis.
    McGinnis W
    PloS one 7.2 (2012): e31365.

    Sex and the single cell. II. There is a time and place for sex.
    Baker BS
    PLoS biology 8.5 (2010 May 4): e1000365.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila.
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

    Developmental competence and the induction of ectopic proboscises in Drosophila melanogaster.
    Teft WA
    Development genes and evolution 223.6 (2013 Nov): 375-387.

    Analysis of the sequence and phenotype of Drosophila Sex combs reduced alleles reveals potential functions of conserved protein motifs of the Sex combs reduced protein.
    Percival-Smith A
    Genetics 182.1 (2009 May): 191-203.

    Drosophila retinal pigment cell death is regulated in a position-dependent manner by a cell memory gene.
    Théodore L
    The International journal of developmental biology 52.1 (2008): 21-31.

    Steroid hormone-dependent transformation of polyhomeotic mutant neurons in the Drosophila brain.
    Lee T
    Development (Cambridge, England) 133.7 (2006 Apr): 1231-40.

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