anti-Gro

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$40.00
SKU: anti-Gro
View product citations for antibody anti-Gro on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Groucho protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Delidakis, C.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Molecular Biology & Biotechnology, FORTH, GREECE
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 80 kDa; Apparent: 80 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: gro
Immunogen: Recombinant Gro (aa 120-380, approximately)/GST fusion protein
Alternate Gene Names: CG8384, Groucho, m9/m10, E(spl)gro, E(spl)m9/10
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: aa 120-380, approximately
Uniprot ID: P16371 
Immunogen Sequence: Partial protein
Entrez Gene ID: 43162 
Additional Characterization:  
Antibody Registry ID: AB_528272 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Gro was deposited to the DSHB by Delidakis, C. (DSHB Hybridoma Product anti-Gro)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    Epitope Map References
    All References

    Two genetically and molecularly distinct functions involved in early neurogenesis reside within the Enhancer of split locus of Drosophila melanogaster.
    Artavanis-Tsakonas S
    Genetics 129.3 (1991 Nov): 803-23.

    Groucho mediates a Ci-independent mechanism of hedgehog repression in the anterior wing pouch.
    Delidakis C
    Development (Cambridge, England) 128.21 (2001 Nov): 4361-70.

    Brinker possesses multiple mechanisms for repression because its primary co-repressor, Groucho, may be unavailable in some cell types.
    Campbell G
    Development (Cambridge, England) 140.20 (2013 Oct): 4256-65.

    The Central Region of the Drosophila Co-repressor Groucho as a Regulatory Hub.
    Courey AJ
    The Journal of biological chemistry 290.50 (2015 Dec 11): 30119-30.

    The Tbx20 homolog Midline represses wingless in conjunction with Groucho during the maintenance of segment polarity.
    Brook WJ
    Developmental biology 369.2 (2012 Sep 15): 319-29.

    The unconserved groucho central region is essential for viability and modulates target gene specificity.
    Courey AJ
    PloS one 7.2 (2012): e30610.

    bHLH proteins involved in Drosophila neurogenesis are mutually regulated at the level of stability.
    Delidakis C
    Nucleic acids research 43.5 (2015 Mar 11): 2543-59.

    Essential roles of Da transactivation domains in neurogenesis and in E(spl)-mediated repression.
    Delidakis C
    Molecular and cellular biology 32.22 (2012 Nov): 4534-48.

    Role for Hes1-induced phosphorylation in Groucho-mediated transcriptional repression.
    Stifani S
    Molecular and cellular biology 22.2 (2002 Jan): 389-99.

    Hairless-mediated repression of notch target genes requires the combined activity of Groucho and CtBP corepressors.
    Preiss A
    Molecular and cellular biology 25.23 (2005 Dec): 10433-41.

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