anti-Gro
Catalog Fields
Antigen: Groucho protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Delidakis, C.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Molecular Biology & Biotechnology, FORTH, GREECE
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Predicted Species Reactivity:
Gene: gro
Immunogen: Recombinant Gro (aa 120-380, approximately)/GST fusion protein
Alternate Gene Names: CG8384, Groucho, m9/m10, E(spl)gro, E(spl)m9/10
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: aa 120-380, approximately
Uniprot ID: P16371
Immunogen Sequence: Partial protein
Entrez Gene ID: 43162
Antibody Registry ID: AB_528272
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Gro was deposited to the DSHB by Delidakis, C. (DSHB Hybridoma Product anti-Gro)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
10 References
Initial Publication
Two genetically and molecularly distinct functions involved in early neurogenesis reside within the Enhancer of split locus of Drosophila melanogaster.
Artavanis-Tsakonas S
Genetics 129.3 (1991 Nov): 803-23.