eya10H6

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$40.00
SKU: eya10H6
View product citations for antibody eya10H6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Eyes Absent (Eya) protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S. / Bonini, N.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila, Xenopus
Depositors Notes: Fusion: 1/28/93.
Antigen Molecular Weight: Predicted; 80 or 81kDa; Apparent: 94kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: eya
Immunogen: Peptide TGRARGRRHQQPSP from Drosophila Eyes Absent (Eya) protein
Alternate Gene Names: eyes absent; cli
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: aa 462-475 (TGRARGRRHQQPSP)
Uniprot ID: Q05201 
Immunogen Sequence: aa 462-475
Entrez Gene ID: 33916 
Additional Characterization:
Antibody Registry ID: AB_528232 
Additional Information: The eya gene product is a tyrosine phosphatase involved in transcription regulation during organogenesis. eya is essential in eye development and is required for the survival of eye progenitor cells.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
eya10H6 was deposited to the DSHB by Benzer, S. / Bonini, N.M. (DSHB Hybridoma Product eya10H6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

49 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Development (Cambridge, England)
    DiNardo S
    Development (Cambridge, England) 124.5 (1997 Mar): 971-82.

    IF References

    The Journal of cell biology
    Pittman RN
    The Journal of cell biology 143.6 (1998 Dec 14): 1457-70.

    Developmental biology
    DiNardo S
    Developmental biology 258.1 (2003 Jun 1): 117-28.

    Developmental biology
    Van Doren M
    Developmental biology 294.1 (2006 Jun 1): 92-103.

    Development (Cambridge, England)
    Van Doren M
    Development (Cambridge, England) 130.18 (2003 Sep): 4417-26.

    Developmental biology
    Noselli S
    Developmental biology 368.1 (2012 Aug 1): 76-85.

    Mechanisms of development
    Van Doren M
    Mechanisms of development 121.11 (2004 Nov): 1323-33.

    Developmental cell
    Deng WM
    Developmental cell 12.3 (2007 Mar): 431-42.

    Development (Cambridge, England)
    Van Doren M
    Development (Cambridge, England) 133.6 (2006 Mar): 1143-53.

    Development (Cambridge, England)
    Mardon G
    Development (Cambridge, England) 130.13 (2003 Jul): 3053-62.

    PLoS biology
    Thor S
    PLoS biology 5.2 (2007 Feb): e37.

    Development (Cambridge, England)
    Van Doren M
    Development (Cambridge, England) 138.16 (2011 Aug): 3357-66.

    Developmental biology
    Van Doren M
    Developmental biology 353.2 (2011 May 15): 217-28.

    Development (Cambridge, England)
    Montell D
    Development (Cambridge, England) 129.23 (2002 Dec): 5377-88.

    Genetics
    Cline TW
    Genetics 180.4 (2008 Dec): 1963-81.

    Developmental biology
    Baker NE
    Developmental biology 327.2 (2009 Mar 15): 366-75.

    Cell stem cell
    Matunis EL
    Cell stem cell 5.2 (2009 Aug 7): 191-203.

    Development (Cambridge, England)
    Thor S
    Development (Cambridge, England) 139.4 (2012 Feb): 678-89.

    Development (Cambridge, England)
    Montell DJ
    Development (Cambridge, England) 131.23 (2004 Dec): 5971-80.

    Development (Cambridge, England)
    Thor S
    Development (Cambridge, England) 138.24 (2011 Dec): 5311-20.

    PloS one
    Mardon G
    PloS one 8.3 (2013): e58818.

    Developmental cell
    Van Doren M
    Developmental cell 14.2 (2008 Feb): 275-86.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Casares F
    Developmental dynamics : an official publication of the American Association of Anatomists 240.1 (2011 Jan): 75-85.

    Developmental biology
    Schulz C
    Developmental biology 394.2 (2014 Oct 15): 217-27.

    Developmental cell
    Schedl P
    Developmental cell 12.2 (2007 Feb): 275-86.

    Proceedings of the National Academy of Sciences of the United States of America
    Montell DJ
    Proceedings of the National Academy of Sciences of the United States of America 110.19 (2013 May 7): E1734-42.

    PloS one
    Mardon G
    PloS one 7.12 (2012): e50776.

    Development (Cambridge, England)
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    PLoS biology
    Thor S
    PLoS biology 8.5 (2010 May 11): e1000368.

    RNA biology
    Förstemann K
    RNA biology 9.5 (2012 May): 633-43.

    Genetics
    Verheyen EM
    Genetics 180.1 (2008 Sep): 283-99.

    Development (Cambridge, England)
    Shilo BZ
    Development (Cambridge, England) 134.6 (2007 Mar): 1161-9.

    Development (Cambridge, England)
    Fuller MT
    Development (Cambridge, England) 141.1 (2014 Jan): 73-82.

    Development (Cambridge, England)
    Thor S
    Development (Cambridge, England) 131.23 (2004 Dec): 5837-48.

    Journal of cellular physiology
    Hou SX
    Journal of cellular physiology 223.2 (2010 May): 500-10.

    Biology open
    Renault AD
    Biology open 1.10 (2012 Oct 15): 1043-8.

    EMBO reports
    Noselli S
    EMBO reports 9.7 (2008 Jul): 676-82.

    PLoS genetics
    Allan DW
    PLoS genetics 8.2 (2012): e1002501.

    Developmental biology
    Du W
    Developmental biology 313.2 (2008 Jan 15): 787-801.

    Developmental biology
    DiNardo S
    Developmental biology 253.2 (2003 Jan 15): 189-99.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Allan DW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.12 (2009 Mar 25): 3852-64.

    Developmental cell
    St Johnston D
    Developmental cell 5.4 (2003 Oct): 547-58.

    Developmental biology
    Pignoni F
    Developmental biology 421.1 (2017 Jan 1): 67-76.

    Nature communications
    Montell DJ
    Nature communications 8.1 (2017 Nov 1): 1244.

    PLoS genetics
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    Development (Cambridge, England)
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    IHC References

    Development (Cambridge, England)
    DiNardo S
    Development (Cambridge, England) 124.5 (1997 Mar): 971-82.

    Development (Cambridge, England)
    Mardon G
    Development (Cambridge, England) 130.13 (2003 Jul): 3053-62.

    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
    Kablar B
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 22.4 (2004 Jun): 215-24.

    PLoS biology
    Benito-Sipos J
    PLoS biology 14.5 (2016 May): e1002450.

    Developmental cell
    Hassan BA
    Developmental cell 50.6 (2019 Sep 23): 780-792.e7.

    All References

    Development (Cambridge, England)
    DiNardo S
    Development (Cambridge, England) 124.5 (1997 Mar): 971-82.

    Development (Cambridge, England)
    Mardon G
    Development (Cambridge, England) 130.13 (2003 Jul): 3053-62.

    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
    Kablar B
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 22.4 (2004 Jun): 215-24.

    PLoS biology
    Benito-Sipos J
    PLoS biology 14.5 (2016 May): e1002450.

    Developmental cell
    Hassan BA
    Developmental cell 50.6 (2019 Sep 23): 780-792.e7.

    The Journal of cell biology
    Pittman RN
    The Journal of cell biology 143.6 (1998 Dec 14): 1457-70.

    Developmental biology
    DiNardo S
    Developmental biology 258.1 (2003 Jun 1): 117-28.

    Developmental biology
    Van Doren M
    Developmental biology 294.1 (2006 Jun 1): 92-103.

    Development (Cambridge, England)
    Van Doren M
    Development (Cambridge, England) 130.18 (2003 Sep): 4417-26.

    Developmental biology
    Noselli S
    Developmental biology 368.1 (2012 Aug 1): 76-85.

    Mechanisms of development
    Van Doren M
    Mechanisms of development 121.11 (2004 Nov): 1323-33.

    Developmental cell
    Deng WM
    Developmental cell 12.3 (2007 Mar): 431-42.

    Development (Cambridge, England)
    Van Doren M
    Development (Cambridge, England) 133.6 (2006 Mar): 1143-53.

    PLoS biology
    Thor S
    PLoS biology 5.2 (2007 Feb): e37.

    Development (Cambridge, England)
    Van Doren M
    Development (Cambridge, England) 138.16 (2011 Aug): 3357-66.

    Developmental biology
    Van Doren M
    Developmental biology 353.2 (2011 May 15): 217-28.

    Development (Cambridge, England)
    Montell D
    Development (Cambridge, England) 129.23 (2002 Dec): 5377-88.

    Genetics
    Cline TW
    Genetics 180.4 (2008 Dec): 1963-81.

    Developmental biology
    Baker NE
    Developmental biology 327.2 (2009 Mar 15): 366-75.

    Cell stem cell
    Matunis EL
    Cell stem cell 5.2 (2009 Aug 7): 191-203.

    Development (Cambridge, England)
    Thor S
    Development (Cambridge, England) 139.4 (2012 Feb): 678-89.

    Development (Cambridge, England)
    Montell DJ
    Development (Cambridge, England) 131.23 (2004 Dec): 5971-80.

    Development (Cambridge, England)
    Thor S
    Development (Cambridge, England) 138.24 (2011 Dec): 5311-20.

    PloS one
    Mardon G
    PloS one 8.3 (2013): e58818.

    Developmental cell
    Van Doren M
    Developmental cell 14.2 (2008 Feb): 275-86.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Casares F
    Developmental dynamics : an official publication of the American Association of Anatomists 240.1 (2011 Jan): 75-85.

    Developmental biology
    Schulz C
    Developmental biology 394.2 (2014 Oct 15): 217-27.

    Developmental cell
    Schedl P
    Developmental cell 12.2 (2007 Feb): 275-86.

    Proceedings of the National Academy of Sciences of the United States of America
    Montell DJ
    Proceedings of the National Academy of Sciences of the United States of America 110.19 (2013 May 7): E1734-42.

    PloS one
    Mardon G
    PloS one 7.12 (2012): e50776.

    Development (Cambridge, England)
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    PLoS biology
    Thor S
    PLoS biology 8.5 (2010 May 11): e1000368.

    RNA biology
    Förstemann K
    RNA biology 9.5 (2012 May): 633-43.

    Genetics
    Verheyen EM
    Genetics 180.1 (2008 Sep): 283-99.

    Development (Cambridge, England)
    Shilo BZ
    Development (Cambridge, England) 134.6 (2007 Mar): 1161-9.

    Development (Cambridge, England)
    Fuller MT
    Development (Cambridge, England) 141.1 (2014 Jan): 73-82.

    Development (Cambridge, England)
    Thor S
    Development (Cambridge, England) 131.23 (2004 Dec): 5837-48.

    Journal of cellular physiology
    Hou SX
    Journal of cellular physiology 223.2 (2010 May): 500-10.

    Biology open
    Renault AD
    Biology open 1.10 (2012 Oct 15): 1043-8.

    EMBO reports
    Noselli S
    EMBO reports 9.7 (2008 Jul): 676-82.

    PLoS genetics
    Allan DW
    PLoS genetics 8.2 (2012): e1002501.

    Developmental biology
    Du W
    Developmental biology 313.2 (2008 Jan 15): 787-801.

    Developmental biology
    DiNardo S
    Developmental biology 253.2 (2003 Jan 15): 189-99.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Allan DW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.12 (2009 Mar 25): 3852-64.

    Developmental cell
    St Johnston D
    Developmental cell 5.4 (2003 Oct): 547-58.

    Developmental biology
    Pignoni F
    Developmental biology 421.1 (2017 Jan 1): 67-76.

    Nature communications
    Montell DJ
    Nature communications 8.1 (2017 Nov 1): 1244.

    PLoS genetics
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    Development (Cambridge, England)
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

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