eya10H6

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$40.00
SKU: eya10H6
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DSHB Data Sheet

Catalog Fields

Antigen: Eyes Absent (Eya) protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S. / Bonini, N.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila, Xenopus
Depositors Notes: Fusion: 1/28/93.
Antigen Molecular Weight: Predicted; 80 or 81kDa; Apparent: 94kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: eya
Immunogen: Peptide TGRARGRRHQQPSP from Drosophila Eyes Absent (Eya) protein
Alternate Gene Names: eyes absent; cli
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: aa 462-475 (TGRARGRRHQQPSP)
Uniprot ID: Q05201 
Immunogen Sequence: aa 462-475
Entrez Gene ID: 33916 
Additional Characterization:  
Antibody Registry ID: AB_528232 
Additional Information: The eya gene product is a tyrosine phosphatase involved in transcription regulation during organogenesis. eya is essential in eye development and is required for the survival of eye progenitor cells.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
eya10H6 was deposited to the DSHB by Benzer, S. / Bonini, N.M. (DSHB Hybridoma Product eya10H6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

49 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Recruitment and the role of nuclear localization in polyglutamine-mediated aggregation.
    Pittman RN
    The Journal of cell biology 143.6 (1998 Dec 14): 1457-70.

    A somatic role for eyes absent (eya) and sine oculis (so) in Drosophila spermatocyte development.
    DiNardo S
    Developmental biology 258.1 (2003 Jun 1): 117-28.

    Development of the male germline stem cell niche in Drosophila.
    Van Doren M
    Developmental biology 294.1 (2006 Jun 1): 92-103.

    Drosophila E-cadherin is essential for proper germ cell-soma interaction during gonad morphogenesis.
    Van Doren M
    Development (Cambridge, England) 130.18 (2003 Sep): 4417-26.

    Drosophila apc regulates delamination of invasive epithelial clusters.
    Noselli S
    Developmental biology 368.1 (2012 Aug 1): 76-85.

    Abdominal-B is essential for proper sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Mechanisms of development 121.11 (2004 Nov): 1323-33.

    Hindsight mediates the role of notch in suppressing hedgehog signaling and cell proliferation.
    Deng WM
    Developmental cell 12.3 (2007 Mar): 431-42.

    Zinc transport activity of Fear of Intimacy is essential for proper gonad morphogenesis and DE-cadherin expression.
    Van Doren M
    Development (Cambridge, England) 133.6 (2006 Mar): 1143-53.

    Mechanism of hedgehog signaling during Drosophila eye development.
    Mardon G
    Development (Cambridge, England) 130.13 (2003 Jul): 3053-62.

    Specification of neuronal identities by feedforward combinatorial coding.
    Thor S
    PLoS biology 5.2 (2007 Feb): e37.

    no child left behind encodes a novel chromatin factor required for germline stem cell maintenance in males but not females.
    Van Doren M
    Development (Cambridge, England) 138.16 (2011 Aug): 3357-66.

    A genetic screen for mutations affecting gonad formation in Drosophila reveals a role for the slit/robo pathway.
    Van Doren M
    Developmental biology 353.2 (2011 May 15): 217-28.

    Eyes absent, a key repressor of polar cell fate during Drosophila oogenesis.
    Montell D
    Development (Cambridge, England) 129.23 (2002 Dec): 5377-88.

    Sexual back talk with evolutionary implications: stimulation of the Drosophila sex-determination gene sex-lethal by its target transformer.
    Cline TW
    Genetics 180.4 (2008 Dec): 1963-81.

    Retinal determination genes as targets and possible effectors of extracellular signals.
    Baker NE
    Developmental biology 327.2 (2009 Mar 15): 366-75.

    Dedifferentiating spermatogonia outcompete somatic stem cells for niche occupancy in the Drosophila testis.
    Matunis EL
    Cell stem cell 5.2 (2009 Aug 7): 191-203.

    Control of neuronal cell fate and number by integration of distinct daughter cell proliferation modes with temporal progression.
    Thor S
    Development (Cambridge, England) 139.4 (2012 Feb): 678-89.

    A role for extra macrochaetae downstream of Notch in follicle cell differentiation.
    Montell DJ
    Development (Cambridge, England) 131.23 (2004 Dec): 5971-80.

    Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development.
    Thor S
    Development (Cambridge, England) 138.24 (2011 Dec): 5311-20.

    Eyes absent tyrosine phosphatase activity is not required for Drosophila development or survival.
    Mardon G
    PloS one 8.3 (2013): e58818.

    Nonautonomous sex determination controls sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Developmental cell 14.2 (2008 Feb): 275-86.

    Regulation of ocellar specification and size by twin of eyeless and homothorax.
    Casares F
    Developmental dynamics : an official publication of the American Association of Anatomists 240.1 (2011 Jan): 75-85.

    Ecdysone signaling opposes epidermal growth factor signaling in regulating cyst differentiation in the male gonad of Drosophila melanogaster.
    Schulz C
    Developmental biology 394.2 (2014 Oct 15): 217-27.

    The master switch gene sex-lethal promotes female development by negatively regulating the N-signaling pathway.
    Schedl P
    Developmental cell 12.2 (2007 Feb): 275-86.

    Castor is required for Hedgehog-dependent cell-fate specification and follicle stem cell maintenance in Drosophila oogenesis.
    Montell DJ
    Proceedings of the National Academy of Sciences of the United States of America 110.19 (2013 May 7): E1734-42.

    MAPK target sites of eyes absent are not required for eye development or survival in Drosophila.
    Mardon G
    PloS one 7.12 (2012): e50776.

    A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes.
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    Segment-specific neuronal subtype specification by the integration of anteroposterior and temporal cues.
    Thor S
    PLoS biology 8.5 (2010 May 11): e1000368.

    Drosophila Pur-α binds to trinucleotide-repeat containing cellular RNAs and translocates to the early oocyte.
    Förstemann K
    RNA biology 9.5 (2012 May): 633-43.

    Drosophila nemo promotes eye specification directed by the retinal determination gene network.
    Verheyen EM
    Genetics 180.1 (2008 Sep): 283-99.

    Drosophila follicle cells are patterned by multiple levels of Notch signaling and antagonism between the Notch and JAK/STAT pathways.
    Shilo BZ
    Development (Cambridge, England) 134.6 (2007 Mar): 1161-9.

    The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells.
    Fuller MT
    Development (Cambridge, England) 141.1 (2014 Jan): 73-82.

    Independent roles of the dachshund and eyes absent genes in BMP signaling, axon pathfinding and neuronal specification.
    Thor S
    Development (Cambridge, England) 131.23 (2004 Dec): 5837-48.

    Competitiveness for the niche and mutual dependence of the germline and somatic stem cells in the Drosophila testis are regulated by the JAK/STAT signaling.
    Hou SX
    Journal of cellular physiology 223.2 (2010 May): 500-10.

    vasa is expressed in somatic cells of the embryonic gonad in a sex-specific manner in Drosophila melanogaster.
    Renault AD
    Biology open 1.10 (2012 Oct 15): 1043-8.

    Drosophila RalA is essential for the maintenance of Jak/Stat signalling in ovarian follicles.
    Noselli S
    EMBO reports 9.7 (2008 Jul): 676-82.

    Developmental transcriptional networks are required to maintain neuronal subtype identity in the mature nervous system.
    Allan DW
    PLoS genetics 8.2 (2012): e1002501.

    Direct control of the proneural gene atonal by retinal determination factors during Drosophila eye development.
    Du W
    Developmental biology 313.2 (2008 Jan 15): 787-801.

    Mutations in Nop60B, the Drosophila homolog of human dyskeratosis congenita 1, affect the maintenance of the germ-line stem cell lineage during spermatogenesis.
    DiNardo S
    Developmental biology 253.2 (2003 Jan 15): 189-99.

    Neuronal phenotype in the mature nervous system is maintained by persistent retrograde bone morphogenetic protein signaling.
    Allan DW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.12 (2009 Mar 25): 3852-64.

    A Notch/Delta-dependent relay mechanism establishes anterior-posterior polarity in Drosophila.
    St Johnston D
    Developmental cell 5.4 (2003 Oct): 547-58.

    Distinct regulation of atonal in a visual organ of Drosophila: Organ-specific enhancer and lack of autoregulation in the larval eye.
    Pignoni F
    Developmental biology 421.1 (2017 Jan 1): 67-76.

    Quantitative microscopy of the Drosophila ovary shows multiple niche signals specify progenitor cell fate.
    Montell DJ
    Nature communications 8.1 (2017 Nov 1): 1244.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    IHC References

    Expression and function of clift in the development of somatic gonadal precursors within the Drosophila mesoderm.
    DiNardo S
    Development (Cambridge, England) 124.5 (1997 Mar): 971-82.

    Mechanism of hedgehog signaling during Drosophila eye development.
    Mardon G
    Development (Cambridge, England) 130.13 (2003 Jul): 3053-62.

    MyoD-lacZ transgenes are early markers in the neural retina, but MyoD function appears to be inhibited in the developing retinal cells.
    Kablar B
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 22.4 (2004 Jun): 215-24.

    Neuronal Cell Fate Specification by the Convergence of Different Spatiotemporal Cues on a Common Terminal Selector Cascade.
    Benito-Sipos J
    PLoS biology 14.5 (2016 May): e1002450.

    Altering the Temporal Regulation of One Transcription Factor Drives Evolutionary Trade-Offs between Head Sensory Organs.
    Hassan BA
    Developmental cell 50.6 (2019 Sep 23): 780-792.e7.

    All References

    Expression and function of clift in the development of somatic gonadal precursors within the Drosophila mesoderm.
    DiNardo S
    Development (Cambridge, England) 124.5 (1997 Mar): 971-82.

    Mechanism of hedgehog signaling during Drosophila eye development.
    Mardon G
    Development (Cambridge, England) 130.13 (2003 Jul): 3053-62.

    MyoD-lacZ transgenes are early markers in the neural retina, but MyoD function appears to be inhibited in the developing retinal cells.
    Kablar B
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 22.4 (2004 Jun): 215-24.

    Neuronal Cell Fate Specification by the Convergence of Different Spatiotemporal Cues on a Common Terminal Selector Cascade.
    Benito-Sipos J
    PLoS biology 14.5 (2016 May): e1002450.

    Altering the Temporal Regulation of One Transcription Factor Drives Evolutionary Trade-Offs between Head Sensory Organs.
    Hassan BA
    Developmental cell 50.6 (2019 Sep 23): 780-792.e7.

    Recruitment and the role of nuclear localization in polyglutamine-mediated aggregation.
    Pittman RN
    The Journal of cell biology 143.6 (1998 Dec 14): 1457-70.

    A somatic role for eyes absent (eya) and sine oculis (so) in Drosophila spermatocyte development.
    DiNardo S
    Developmental biology 258.1 (2003 Jun 1): 117-28.

    Development of the male germline stem cell niche in Drosophila.
    Van Doren M
    Developmental biology 294.1 (2006 Jun 1): 92-103.

    Drosophila E-cadherin is essential for proper germ cell-soma interaction during gonad morphogenesis.
    Van Doren M
    Development (Cambridge, England) 130.18 (2003 Sep): 4417-26.

    Drosophila apc regulates delamination of invasive epithelial clusters.
    Noselli S
    Developmental biology 368.1 (2012 Aug 1): 76-85.

    Abdominal-B is essential for proper sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Mechanisms of development 121.11 (2004 Nov): 1323-33.

    Hindsight mediates the role of notch in suppressing hedgehog signaling and cell proliferation.
    Deng WM
    Developmental cell 12.3 (2007 Mar): 431-42.

    Zinc transport activity of Fear of Intimacy is essential for proper gonad morphogenesis and DE-cadherin expression.
    Van Doren M
    Development (Cambridge, England) 133.6 (2006 Mar): 1143-53.

    Specification of neuronal identities by feedforward combinatorial coding.
    Thor S
    PLoS biology 5.2 (2007 Feb): e37.

    no child left behind encodes a novel chromatin factor required for germline stem cell maintenance in males but not females.
    Van Doren M
    Development (Cambridge, England) 138.16 (2011 Aug): 3357-66.

    A genetic screen for mutations affecting gonad formation in Drosophila reveals a role for the slit/robo pathway.
    Van Doren M
    Developmental biology 353.2 (2011 May 15): 217-28.

    Eyes absent, a key repressor of polar cell fate during Drosophila oogenesis.
    Montell D
    Development (Cambridge, England) 129.23 (2002 Dec): 5377-88.

    Sexual back talk with evolutionary implications: stimulation of the Drosophila sex-determination gene sex-lethal by its target transformer.
    Cline TW
    Genetics 180.4 (2008 Dec): 1963-81.

    Retinal determination genes as targets and possible effectors of extracellular signals.
    Baker NE
    Developmental biology 327.2 (2009 Mar 15): 366-75.

    Dedifferentiating spermatogonia outcompete somatic stem cells for niche occupancy in the Drosophila testis.
    Matunis EL
    Cell stem cell 5.2 (2009 Aug 7): 191-203.

    Control of neuronal cell fate and number by integration of distinct daughter cell proliferation modes with temporal progression.
    Thor S
    Development (Cambridge, England) 139.4 (2012 Feb): 678-89.

    A role for extra macrochaetae downstream of Notch in follicle cell differentiation.
    Montell DJ
    Development (Cambridge, England) 131.23 (2004 Dec): 5971-80.

    Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development.
    Thor S
    Development (Cambridge, England) 138.24 (2011 Dec): 5311-20.

    Eyes absent tyrosine phosphatase activity is not required for Drosophila development or survival.
    Mardon G
    PloS one 8.3 (2013): e58818.

    Nonautonomous sex determination controls sexually dimorphic development of the Drosophila gonad.
    Van Doren M
    Developmental cell 14.2 (2008 Feb): 275-86.

    Regulation of ocellar specification and size by twin of eyeless and homothorax.
    Casares F
    Developmental dynamics : an official publication of the American Association of Anatomists 240.1 (2011 Jan): 75-85.

    Ecdysone signaling opposes epidermal growth factor signaling in regulating cyst differentiation in the male gonad of Drosophila melanogaster.
    Schulz C
    Developmental biology 394.2 (2014 Oct 15): 217-27.

    The master switch gene sex-lethal promotes female development by negatively regulating the N-signaling pathway.
    Schedl P
    Developmental cell 12.2 (2007 Feb): 275-86.

    Castor is required for Hedgehog-dependent cell-fate specification and follicle stem cell maintenance in Drosophila oogenesis.
    Montell DJ
    Proceedings of the National Academy of Sciences of the United States of America 110.19 (2013 May 7): E1734-42.

    MAPK target sites of eyes absent are not required for eye development or survival in Drosophila.
    Mardon G
    PloS one 7.12 (2012): e50776.

    A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes.
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    Segment-specific neuronal subtype specification by the integration of anteroposterior and temporal cues.
    Thor S
    PLoS biology 8.5 (2010 May 11): e1000368.

    Drosophila Pur-α binds to trinucleotide-repeat containing cellular RNAs and translocates to the early oocyte.
    Förstemann K
    RNA biology 9.5 (2012 May): 633-43.

    Drosophila nemo promotes eye specification directed by the retinal determination gene network.
    Verheyen EM
    Genetics 180.1 (2008 Sep): 283-99.

    Drosophila follicle cells are patterned by multiple levels of Notch signaling and antagonism between the Notch and JAK/STAT pathways.
    Shilo BZ
    Development (Cambridge, England) 134.6 (2007 Mar): 1161-9.

    The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells.
    Fuller MT
    Development (Cambridge, England) 141.1 (2014 Jan): 73-82.

    Independent roles of the dachshund and eyes absent genes in BMP signaling, axon pathfinding and neuronal specification.
    Thor S
    Development (Cambridge, England) 131.23 (2004 Dec): 5837-48.

    Competitiveness for the niche and mutual dependence of the germline and somatic stem cells in the Drosophila testis are regulated by the JAK/STAT signaling.
    Hou SX
    Journal of cellular physiology 223.2 (2010 May): 500-10.

    vasa is expressed in somatic cells of the embryonic gonad in a sex-specific manner in Drosophila melanogaster.
    Renault AD
    Biology open 1.10 (2012 Oct 15): 1043-8.

    Drosophila RalA is essential for the maintenance of Jak/Stat signalling in ovarian follicles.
    Noselli S
    EMBO reports 9.7 (2008 Jul): 676-82.

    Developmental transcriptional networks are required to maintain neuronal subtype identity in the mature nervous system.
    Allan DW
    PLoS genetics 8.2 (2012): e1002501.

    Direct control of the proneural gene atonal by retinal determination factors during Drosophila eye development.
    Du W
    Developmental biology 313.2 (2008 Jan 15): 787-801.

    Mutations in Nop60B, the Drosophila homolog of human dyskeratosis congenita 1, affect the maintenance of the germ-line stem cell lineage during spermatogenesis.
    DiNardo S
    Developmental biology 253.2 (2003 Jan 15): 189-99.

    Neuronal phenotype in the mature nervous system is maintained by persistent retrograde bone morphogenetic protein signaling.
    Allan DW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.12 (2009 Mar 25): 3852-64.

    A Notch/Delta-dependent relay mechanism establishes anterior-posterior polarity in Drosophila.
    St Johnston D
    Developmental cell 5.4 (2003 Oct): 547-58.

    Distinct regulation of atonal in a visual organ of Drosophila: Organ-specific enhancer and lack of autoregulation in the larval eye.
    Pignoni F
    Developmental biology 421.1 (2017 Jan 1): 67-76.

    Quantitative microscopy of the Drosophila ovary shows multiple niche signals specify progenitor cell fate.
    Montell DJ
    Nature communications 8.1 (2017 Nov 1): 1244.

    Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye.
    Treisman JE
    PLoS genetics 14.1 (2018 Jan): e1007173.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

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