Antigen: Eyes Absent (Eya) protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S. / Bonini, N.M.
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila, Xenopus
Depositors Notes: Fusion: 1/28/93.
Antigen Molecular Weight: Predicted; 80 or 81kDa; Apparent: 94kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Peptide TGRARGRRHQQPSP from Drosophila Eyes Absent (Eya) protein
Alternate Gene Names: eyes absent; cli
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: aa 462-475 (TGRARGRRHQQPSP)
Uniprot ID: Q05201
Immunogen Sequence: aa 462-475
Entrez Gene ID: 33916
Antibody Registry ID: AB_528232
Additional Information: The eya gene product is a tyrosine phosphatase involved in transcription regulation during organogenesis. eya is essential in eye development and is required for the survival of eye progenitor cells.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
eya10H6 was deposited to the DSHB by Benzer, S. / Bonini, N.M. (DSHB Hybridoma Product eya10H6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).