kel 1B
Catalog Fields
Antigen: Kelch
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Cooley, L.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila, Drosophila melanogaster, Xenopus laevis
Depositors Institution: Yale Medical School
Antigen Molecular Weight: Predicted: 77 kDa (short), 160 kDa (full lenght); Apparent: ~80 kDa (short), ~180 kDa (full length)
Depositors Notes: Use 1:10 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or 1:1 in PBT (PBS, 0.3% Triton-X-100, and 0.5% BSA) for ovary staining. (Xue and Cooley, 1993). This antibody doesn't appear to work for immunoprecipitation. No immunoreactivity in mutant fly.
Immunogen: Recombinant Kelch (first 330 aa of N-terminus)/GST fusion protein
Gene: kel
Alternate Antibody Name: 9C, kelch 1B
Alternate Gene Names: CG7210, Kelch
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: Yes
Uniprot ID: Q04652
Epitope Location or Sequence: BTB domain (aa 111-230)
Entrez Gene ID: 35084
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528318
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: Kelch is a component of ring canals that regulates the flow of cytoplasm between cells. kel 1B recognizes both the short and full lenght Kelch protein.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
kel 1B was deposited to the DSHB by Cooley, L. (DSHB Hybridoma Product kel 1B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
kelch encodes a component of intercellular bridges in Drosophila egg chambers.
Cooley L
Cell 72.5 (1993 Mar 12): 681-93.