kel 1B

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$40.00
SKU: kel 1B
View product citations for antibody kel 1B on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Kelch
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Cooley, L.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale Medical School
Positive Tested Species Reactivity: Drosophila, Drosophila melanogaster, Xenopus laevis
Depositors Notes: Use 1:10 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or 1:1 in PBT (PBS, 0.3% Triton-X-100, and 0.5% BSA) for ovary staining. (Xue and Cooley, 1993). This antibody doesn't appear to work for immunoprecipitation. No immunoreactivity in mutant fly.
Antigen Molecular Weight: Predicted: 77 kDa (short), 160 kDa (full lenght); Apparent: ~80 kDa (short), ~180 kDa (full length)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: kel
Immunogen: Recombinant Kelch (first 330 aa of N-terminus)/GST fusion protein
Alternate Gene Names: CG7210, Kelch
Alternate Antibody Name: 9C, kelch 1B
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: BTB domain (aa 111-230)
Uniprot ID: Q04652 
Immunogen Sequence: Partial protein
Entrez Gene ID: 35084 
Additional Characterization:  
Antibody Registry ID: AB_528318 
Additional Information: Kelch is a component of ring canals that regulates the flow of cytoplasm between cells. kel 1B recognizes both the short and full lenght Kelch protein.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
kel 1B was deposited to the DSHB by Cooley, L. (DSHB Hybridoma Product kel 1B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    kelch encodes a component of intercellular bridges in Drosophila egg chambers.
    Cooley L
    Cell 72.5 (1993 Mar 12): 681-93.

    Drosophila kelch is an oligomeric ring canal actin organizer.
    Cooley L
    The Journal of cell biology 138.4 (1997 Aug 25): 799-810.

    Morphogenesis of Drosophila ovarian ring canals.
    Cooley L
    Development (Cambridge, England) 120.7 (1994 Jul): 2015-25.

    Examination of the function of two kelch proteins generated by stop codon suppression.
    Cooley L
    Development (Cambridge, England) 124.7 (1997 Apr): 1405-17.

    Drosophila Kelch regulates actin organization via Src64-dependent tyrosine phosphorylation.
    Cooley L
    The Journal of cell biology 156.4 (2002 Feb 18): 703-13.

    Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeleton.
    Cooley L
    The Journal of cell biology 188.1 (2010 Jan 11): 29-37.

    Actin Cytoskeletal Organization in Drosophila Germline Ring Canals Depends on Kelch Function in a Cullin-RING E3 Ligase.
    Cooley L
    Genetics 201.3 (2015 Nov): 1117-31.

    Formation, architecture and polarity of female germline cyst in Xenopus.
    Etkin LD
    Developmental biology 266.1 (2004 Feb 1): 43-61.

    The insulator protein Suppressor of Hairy wing is required for proper ring canal development during oogenesis in Drosophila.
    Labrador M
    Developmental biology 403.1 (2015 Jul 1): 57-68.

    Capping protein beta is required for actin cytoskeleton organisation and cell migration during Drosophila oogenesis.
    Baricheva EM
    Cell biology international 37.2 (2013 Feb): 149-59.

    Evidence for the mechanosensor function of filamin in tissue development.
    Ylänne J
    Scientific reports 6. (2016 Sep 6): 32798.

    The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.
    Lewellyn L
    Developmental biology 440.2 (2018 Aug 15): 99-112.

    WB References
    IHC References

    Formation, architecture and polarity of female germline cyst in Xenopus.
    Etkin LD
    Developmental biology 266.1 (2004 Feb 1): 43-61.

    Epitope Map References

    Drosophila kelch is an oligomeric ring canal actin organizer.
    Cooley L
    The Journal of cell biology 138.4 (1997 Aug 25): 799-810.

    All References

    Formation, architecture and polarity of female germline cyst in Xenopus.
    Etkin LD
    Developmental biology 266.1 (2004 Feb 1): 43-61.

    kelch encodes a component of intercellular bridges in Drosophila egg chambers.
    Cooley L
    Cell 72.5 (1993 Mar 12): 681-93.

    Drosophila kelch is an oligomeric ring canal actin organizer.
    Cooley L
    The Journal of cell biology 138.4 (1997 Aug 25): 799-810.

    Morphogenesis of Drosophila ovarian ring canals.
    Cooley L
    Development (Cambridge, England) 120.7 (1994 Jul): 2015-25.

    Examination of the function of two kelch proteins generated by stop codon suppression.
    Cooley L
    Development (Cambridge, England) 124.7 (1997 Apr): 1405-17.

    Drosophila Kelch regulates actin organization via Src64-dependent tyrosine phosphorylation.
    Cooley L
    The Journal of cell biology 156.4 (2002 Feb 18): 703-13.

    Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeleton.
    Cooley L
    The Journal of cell biology 188.1 (2010 Jan 11): 29-37.

    Actin Cytoskeletal Organization in Drosophila Germline Ring Canals Depends on Kelch Function in a Cullin-RING E3 Ligase.
    Cooley L
    Genetics 201.3 (2015 Nov): 1117-31.

    The insulator protein Suppressor of Hairy wing is required for proper ring canal development during oogenesis in Drosophila.
    Labrador M
    Developmental biology 403.1 (2015 Jul 1): 57-68.

    Capping protein beta is required for actin cytoskeleton organisation and cell migration during Drosophila oogenesis.
    Baricheva EM
    Cell biology international 37.2 (2013 Feb): 149-59.

    Evidence for the mechanosensor function of filamin in tissue development.
    Ylänne J
    Scientific reports 6. (2016 Sep 6): 32798.

    The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.
    Lewellyn L
    Developmental biology 440.2 (2018 Aug 15): 99-112.

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