Antibody Name: kel 1B
Antigen:Kelch
Immunofluorescence image of a compound egg chamber from a sec3GT mutant Drosophila stained with DSHB antibody kel 1B against Kelch (green). PMID: 30984026, Fig. 1E.
Catalog Fields
Identity
Clone ID / Antibody Name: kel 1B
Alternate Antibody Name: 9C, kelch 1B
Antigen Name: Kelch
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: kel
Entrez Gene ID: 35084
Antigen Sequence: —
Antigen Species: Drosophila
Antigen Mol. Weight: Predicted: 77 kDa (short), 160 kDa (full lenght); Apparent: ~80 kDa (short), ~180 kDa (full length)
Uniprot ID: Q04652
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1, kappa light chain
Myeloma Strain: —
Antibody Registry / RRID: AB_528318
Available to For-Profits: No
Hybridoma Available: No
Immunogen & Epitope
Immunogen: Recombinant Kelch (first 330 aa of N-terminus)/GST fusion protein
Immunogen Sequence: Partial protein
Epitope Mapped: Yes
Epitope Location / Seq: BTB domain (aa 111-230)
Clone: —
Additional Info: Kelch is a component of ring canals that regulates the flow of cytoplasm between cells. kel 1B recognizes both the short and full lenght Kelch protein.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Drosophila, Drosophila melanogaster, Xenopus laevis
Predicted Reactivity: —
Depositor
Depositor Name: L. Cooley
Institution: Yale Medical School
Deposit Date: July 12, 2001
Depositor Notes: Use 1:10 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or 1:1 in PBT (PBS, 0.3% Triton-X-100, and 0.5% BSA) for ovary staining. (Xue and Cooley, 1993). This antibody doesn't appear to work for immunoprecipitation. No immunoreactivity in mutant fly.
Recommended Applications
IF
IHC
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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