kel 1B
Catalog Fields
Clone ID/Product Name: kel 1B
Available to For-Profits: No
Alternate Antibody Name: 9C, kelch 1B
Gene Symbol: kel
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 35084
Clonality: Monoclonal
Immunogen: Recombinant Kelch (first 330 aa of N-terminus)/GST fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: Kelch
Epitope Location or Sequence: BTB domain (aa 111-230)
Alternate Antigen Name:
Deposit Date: 7/12/2001
Antigen Molecular Weight: Predicted: 77 kDa (short), 160 kDa (full lenght); Apparent: ~80 kDa (short), ~180 kDa (full length)
Depositor: Cooley, L.
Antigen Sequence:
Depositor Institution: Yale Medical School
Antigen Species: Drosophila
Depositor Notes: Use 1:10 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or 1:1 in PBT (PBS, 0.3% Triton-X-100, and 0.5% BSA) for ovary staining. (Xue and Cooley, 1993). This antibody doesn't appear to work for immunoprecipitation. No immunoreactivity in mutant fly.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila, Drosophila melanogaster, Xenopus laevis
Additional Information: Kelch is a component of ring canals that regulates the flow of cytoplasm between cells. kel 1B recognizes both the short and full lenght Kelch protein.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot