anti-vasa

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$40.00
SKU: anti-vasa
View product citations for antibody anti-vasa on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: vasa
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Spradling, A.C. / Williams, D.
Isotype: RIgM, kappa light chain
Depositors Institution: Carnegie Institute of Washington
Host Species: rat
Gene: vas
Positive Tested Species Reactivity: Drosophila
Alternate Gene Names: CG46283
Antigen Molecular Weight: Predicted: 72 kDa; Apparent 72 kDa
Clonality: Monoclonal
Immunogen: Recombinant fusion protein GST-vasa
Epitope Mapped: No
Uniprot ID: P09052 
Immunogen Sequence: Full length protein
Entrez Gene ID: 26067080 
Additional Information: Vasa is a germline specific marker
Antibody Registry ID: AB_760351 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-vasa was deposited to the DSHB by Spradling, A.C. / Williams, D. (DSHB Hybridoma Product anti-vasa)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

17 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Regulation of cell growth by Notch signaling and its differential requirement in normal vs. tumor-forming stem cells in Drosophila.
    Lu B
    Genes & development 25.24 (2011 Dec 15): 2644-58.

    vasa is expressed in somatic cells of the embryonic gonad in a sex-specific manner in Drosophila melanogaster.
    Renault AD
    Biology open 1.10 (2012 Oct 15): 1043-8.

    Germline progenitors escape the widespread phenomenon of homolog pairing during Drosophila development.
    Wu CT
    PLoS genetics 9.12 (2013): e1004013.

    Identification of seven genes essential for male fertility through a genome-wide association study of non-obstructive azoospermia and RNA interference-mediated large-scale functional screening in Drosophila.
    Tong C
    Human molecular genetics 24.5 (2015 Mar 1): 1493-503.

    Histone H3K9 trimethylase Eggless controls germline stem cell maintenance and differentiation.
    Xie T
    PLoS genetics 7.12 (2011 Dec): e1002426.

    Reduced fertility of Drosophila melanogaster hybrid male rescue (Hmr) mutant females is partially complemented by Hmr orthologs from sibling species.
    Barbash DA
    Genetics 181.4 (2009 Apr): 1437-50.

    RNA helicase Belle/DDX3 regulates transgene expression in Drosophila.
    Deng WM
    Developmental biology 412.1 (2016 Apr 1): 57-70.

    The Hmr and Lhr hybrid incompatibility genes suppress a broad range of heterochromatic repeats.
    Barbash DA
    PLoS genetics 10.3 (2014 Mar): e1004240.

    Protein competition switches the function of COP9 from self-renewal to differentiation.
    Xie T
    Nature 514.7521 (2014 Oct 9): 233-6.

    Drosophila piwi mutants exhibit germline stem cell tumors that are sustained by elevated Dpp signaling.
    Lai EC
    Current biology : CB 23.15 (2013 Aug 5): 1442-8.

    Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar.
    Pai LM
    Development (Cambridge, England) 141.3 (2014 Feb): 563-73.

    Dorso-ventral axis formation of the Drosophila oocyte requires Cyclin G.
    Preiss A
    Hereditas 149.5 (2012 Oct): 186-96.

    Germ cell connectivity enhances cell death in response to DNA damage in the <i>Drosophila</i> testis.
    Yamashita YM
    eLife 6. (2017 Aug 15): .

    <i>Drosophila small ovary</i> gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation.
    Erdélyi M
    Development (Cambridge, England) 145.23 (2018 Dec 4): .

    WB References

    Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling.
    Tootle TL
    Molecular biology of the cell 23.23 (2012 Dec): 4567-78.

    The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis.
    Tootle TL
    Developmental biology 393.2 (2014 Sep 15): 209-226.

    Cyclin G is involved in meiotic recombination repair in Drosophila melanogaster.
    Preiss A
    Journal of cell science 125.Pt 22 (2012 Nov 15): 5555-63.

    IHC References
    All References

    vasa is expressed in somatic cells of the embryonic gonad in a sex-specific manner in Drosophila melanogaster.
    Renault AD
    Biology open 1.10 (2012 Oct 15): 1043-8.

    Regulation of cell growth by Notch signaling and its differential requirement in normal vs. tumor-forming stem cells in Drosophila.
    Lu B
    Genes & development 25.24 (2011 Dec 15): 2644-58.

    Germline progenitors escape the widespread phenomenon of homolog pairing during Drosophila development.
    Wu CT
    PLoS genetics 9.12 (2013): e1004013.

    Identification of seven genes essential for male fertility through a genome-wide association study of non-obstructive azoospermia and RNA interference-mediated large-scale functional screening in Drosophila.
    Tong C
    Human molecular genetics 24.5 (2015 Mar 1): 1493-503.

    Histone H3K9 trimethylase Eggless controls germline stem cell maintenance and differentiation.
    Xie T
    PLoS genetics 7.12 (2011 Dec): e1002426.

    Reduced fertility of Drosophila melanogaster hybrid male rescue (Hmr) mutant females is partially complemented by Hmr orthologs from sibling species.
    Barbash DA
    Genetics 181.4 (2009 Apr): 1437-50.

    RNA helicase Belle/DDX3 regulates transgene expression in Drosophila.
    Deng WM
    Developmental biology 412.1 (2016 Apr 1): 57-70.

    The Hmr and Lhr hybrid incompatibility genes suppress a broad range of heterochromatic repeats.
    Barbash DA
    PLoS genetics 10.3 (2014 Mar): e1004240.

    Protein competition switches the function of COP9 from self-renewal to differentiation.
    Xie T
    Nature 514.7521 (2014 Oct 9): 233-6.

    Drosophila piwi mutants exhibit germline stem cell tumors that are sustained by elevated Dpp signaling.
    Lai EC
    Current biology : CB 23.15 (2013 Aug 5): 1442-8.

    Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar.
    Pai LM
    Development (Cambridge, England) 141.3 (2014 Feb): 563-73.

    Dorso-ventral axis formation of the Drosophila oocyte requires Cyclin G.
    Preiss A
    Hereditas 149.5 (2012 Oct): 186-96.

    Germ cell connectivity enhances cell death in response to DNA damage in the <i>Drosophila</i> testis.
    Yamashita YM
    eLife 6. (2017 Aug 15): .

    <i>Drosophila small ovary</i> gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation.
    Erdélyi M
    Development (Cambridge, England) 145.23 (2018 Dec 4): .

    Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling.
    Tootle TL
    Molecular biology of the cell 23.23 (2012 Dec): 4567-78.

    The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis.
    Tootle TL
    Developmental biology 393.2 (2014 Sep 15): 209-226.

    Cyclin G is involved in meiotic recombination repair in Drosophila melanogaster.
    Preiss A
    Journal of cell science 125.Pt 22 (2012 Nov 15): 5555-63.

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