Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Spradling, A.C. / Williams, D.
Isotype: RIgM, kappa light chain
Host Species: rat
Depositors Institution: Carnegie Institute of Washington
Positive Tested Species Reactivity: Drosophila
Antigen Molecular Weight: Predicted: 72 kDa; Apparent 72 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant fusion protein GST-vasa
Alternate Gene Names: CG46283
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Location or Sequence:
Uniprot ID: P09052
Immunogen Sequence: Full length protein
Entrez Gene ID: 26067080
Antibody Registry ID: AB_760351
Additional Information: Vasa is a germline specific marker
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-vasa was deposited to the DSHB by Spradling, A.C. / Williams, D. (DSHB Hybridoma Product anti-vasa)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).