hts RC

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$40.00
SKU: hts RC
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DSHB Data Sheet

Catalog Fields

Antigen: hu-li tai shao, hts-RC
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Cooley, L.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale Medical School
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Use 1:10 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or 1:1 in PBT (PBS, 0.3% Triton-X-100, and 0.5% BSA) for ovary staining. (Robinson et al., 1994). Identifies an epitope on the ring canal. Doesn’t work for Immunoprecipitation.
Antigen Molecular Weight: Apparent: 55 and 68 kDa have been reported
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: hts
Immunogen: Recombinant Drosophila hu-li tai shao protein (351 aa C-terminus of hts, isoform N4)/GST fusion.
Alternate Gene Names: CG43443, Add, adducin, hu li tai shao, add-like, add-hts, Ovhts
Alternate Antibody Name: anti-hts 655 4B
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: 351 aa C-terminal of isoform A (N4; Uniprot ID: Q02645-1) spanning aa 806-1156.
Uniprot ID: Q02645 
Immunogen Sequence: Partial protein
Entrez Gene ID: 37230 
Additional Characterization:  
Antibody Registry ID: AB_528289 
Additional Information: hts RC will not recognize any isoform missing the C-terminal portion of isoform N4. Rcognizes Ovhts-RC.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
hts RC was deposited to the DSHB by Cooley, L. (DSHB Hybridoma Product hts RC)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Morphogenesis of Drosophila ovarian ring canals.
    Cooley L
    Development (Cambridge, England) 120.7 (1994 Jul): 2015-25.

    IF References

    Morphogenesis of Drosophila ovarian ring canals.
    Cooley L
    Development (Cambridge, England) 120.7 (1994 Jul): 2015-25.

    Src64 is required for ovarian ring canal morphogenesis during Drosophila oogenesis.
    Simon MA
    Development (Cambridge, England) 125.15 (1998 Aug): 2883-92.

    Examination of the function of two kelch proteins generated by stop codon suppression.
    Cooley L
    Development (Cambridge, England) 124.7 (1997 Apr): 1405-17.

    Formation of the Drosophila ovarian ring canal inner rim depends on cheerio.
    Cooley L
    Genetics 145.4 (1997 Apr): 1063-72.

    Partial Functional Diversification of Drosophila melanogaster Septin Genes Sep2 and Sep5.
    Clark DV
    G3 (Bethesda, Md.) 6.7 (2016 Jul 7): 1947-57.

    Antibody Staining in Drosophila Germaria.
    Haglund K
    Methods in molecular biology (Clifton, N.J.) 1457. (2016): 19-33.

    The insulator protein Suppressor of Hairy wing is required for proper ring canal development during oogenesis in Drosophila.
    Labrador M
    Developmental biology 403.1 (2015 Jul 1): 57-68.

    Drosophila kelch is an oligomeric ring canal actin organizer.
    Cooley L
    The Journal of cell biology 138.4 (1997 Aug 25): 799-810.

    Drosophila non-muscle alpha-actinin is localized in nurse cell actin bundles and ring canals, but is not required for fertility.
    Heino TI
    Mechanisms of development 121.11 (2004 Nov): 1377-91.

    Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in Drosophila oogenesis.
    Wodarz A
    The Journal of cell biology 193.1 (2011 Apr 4): 71-80.

    Domains of Importin-alpha2 required for ring canal assembly during Drosophila oogenesis.
    Mechler BM
    Journal of structural biology 154.1 (2006 Apr): 27-41.

    Drosophila Argonaute 1 and its miRNA biogenesis partners are required for oocyte formation and germline cell division.
    Liu JL
    Developmental biology 365.2 (2012 May 15): 384-94.

    Mutations in the catalytic loop HRD motif alter the activity and function of Drosophila Src64.
    Thomas JH
    PloS one 6.11 (2011): e28100.

    The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.
    Lewellyn L
    Developmental biology 440.2 (2018 Aug 15): 99-112.

    WB References

    Morphogenesis of Drosophila ovarian ring canals.
    Cooley L
    Development (Cambridge, England) 120.7 (1994 Jul): 2015-25.

    Examination of the function of two kelch proteins generated by stop codon suppression.
    Cooley L
    Development (Cambridge, England) 124.7 (1997 Apr): 1405-17.

    Formation of the Drosophila ovarian ring canal inner rim depends on cheerio.
    Cooley L
    Genetics 145.4 (1997 Apr): 1063-72.

    Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in Drosophila oogenesis.
    Wodarz A
    The Journal of cell biology 193.1 (2011 Apr 4): 71-80.

    All References

    Morphogenesis of Drosophila ovarian ring canals.
    Cooley L
    Development (Cambridge, England) 120.7 (1994 Jul): 2015-25.

    Src64 is required for ovarian ring canal morphogenesis during Drosophila oogenesis.
    Simon MA
    Development (Cambridge, England) 125.15 (1998 Aug): 2883-92.

    Examination of the function of two kelch proteins generated by stop codon suppression.
    Cooley L
    Development (Cambridge, England) 124.7 (1997 Apr): 1405-17.

    Formation of the Drosophila ovarian ring canal inner rim depends on cheerio.
    Cooley L
    Genetics 145.4 (1997 Apr): 1063-72.

    Partial Functional Diversification of Drosophila melanogaster Septin Genes Sep2 and Sep5.
    Clark DV
    G3 (Bethesda, Md.) 6.7 (2016 Jul 7): 1947-57.

    Antibody Staining in Drosophila Germaria.
    Haglund K
    Methods in molecular biology (Clifton, N.J.) 1457. (2016): 19-33.

    The insulator protein Suppressor of Hairy wing is required for proper ring canal development during oogenesis in Drosophila.
    Labrador M
    Developmental biology 403.1 (2015 Jul 1): 57-68.

    Drosophila kelch is an oligomeric ring canal actin organizer.
    Cooley L
    The Journal of cell biology 138.4 (1997 Aug 25): 799-810.

    Drosophila non-muscle alpha-actinin is localized in nurse cell actin bundles and ring canals, but is not required for fertility.
    Heino TI
    Mechanisms of development 121.11 (2004 Nov): 1377-91.

    Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in Drosophila oogenesis.
    Wodarz A
    The Journal of cell biology 193.1 (2011 Apr 4): 71-80.

    Domains of Importin-alpha2 required for ring canal assembly during Drosophila oogenesis.
    Mechler BM
    Journal of structural biology 154.1 (2006 Apr): 27-41.

    Drosophila Argonaute 1 and its miRNA biogenesis partners are required for oocyte formation and germline cell division.
    Liu JL
    Developmental biology 365.2 (2012 May 15): 384-94.

    Mutations in the catalytic loop HRD motif alter the activity and function of Drosophila Src64.
    Thomas JH
    PloS one 6.11 (2011): e28100.

    The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.
    Lewellyn L
    Developmental biology 440.2 (2018 Aug 15): 99-112.

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