Antigen: hu-li tai shao, not hts-PC
Hybridoma Cells Available:
Antigen Species: Drosophila
Depositor: Lipshitz, H.D.
Host Species: mouse
Depositors Institution: Hospital for Sick Children
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antigen is also known as adducin-like protein.
Antigen Molecular Weight: Multiple molecular weights due to multiple isoforms: the drosophila Adducin-like protein was initially reported to be 87 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: GST-adducin fusion protein expressed in E. coli
Alternate Gene Names: add; Add; Add-hts; add-like; adducin; hu li tai shao; Ovhts; CG43443
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: aa 472-658 of isoform N4 (see additional information)
Uniprot ID: Q02645
Immunogen Sequence: aa. 465-903 Of N4 isoform [Uniprot ID Q02645-1]
Entrez Gene ID: 37230
Antibody Registry ID: AB_528070
Additional Information: 1B1 recognizes all isoforms produced by alternative splicing except ShAdd (also known as hts-PC or N32/R2 [Uniprot ID Q02645-3]). By deduction, the 1B1 epitope is located in the region of N4 [Uniprot ID Q02645-1] common to all isoforms but absent in ShAdd, and corresponds to aa 472-658 of isoform N4. 1B1 recognizes only one fragment resulting from proteolysis of Ovhts (N4): Ovhts Fus is recognized but Ovhts-RC is not.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1B1 was deposited to the DSHB by Lipshitz, H.D. (DSHB Hybridoma Product 1B1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References