Clone ID / Name: 1B1
Anti - Hu-li tai shao, not hts-PC
IF of Drosophila melanogaster germarium stained with DSHB antibody anti-vasa against vasa(green), DSHB antibody 1B1 against hts (magenta), phalloidin (red), DAPI (blue). Image credit: Joana B Santos,
Catalog Fields
Identity
Clone ID / Name: 1B1
Alternate Antibody Name: —
Antigen Name: Hu-li tai shao, not hts-PC
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: hts
Entrez Gene ID: 37230
Antigen Sequence: —
Antigen Species: Drosophila
Antigen Mol. Weight: Multiple molecular weights due to multiple isoforms: the drosophila Adducin-like protein was initially reported to be 87 kDa
Uniprot ID: Q02645
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: HL-1
Antibody Registry / RRID: AB_528070
Available to For-Profits: Yes
Hybridoma Available: No
Immunogen & Epitope
Immunogen: GST-adducin fusion protein expressed in E. coli
Immunogen Sequence: aa. 465-903 Of N4 isoform [Uniprot ID Q02645-1]
Epitope Mapped: Yes
Epitope Location / Seq: aa 472-658 of isoform N4 (see additional information)
Clone: —
Additional Info: 1B1 recognizes all isoforms produced by alternative splicing except ShAdd (also known as hts-PC or N32/R2 [Uniprot ID Q02645-3]). By deduction, the 1B1 epitope is located in the region of N4 [Uniprot ID Q02645-1] common to all isoforms but absent in ShAdd, and corresponds to aa 472-658 of isoform N4. 1B1 recognizes only one fragment resulting from proteolysis of Ovhts (N4): Ovhts Fus is recognized but Ovhts-RC is not.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Drosophila
Predicted Reactivity: —
Depositor
Depositor Name: H.D. Lipshitz
Institution: Hospital for Sick Children
Deposit Date: December 2, 1997
Depositor Notes: This antigen is also known as adducin-like protein.
Recommended Applications
IF
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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