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SKU: 1B1
View product citations for antibody 1B1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: hu-li tai shao, not hts-PC
Hybridoma Cells Available:
Antigen Species: Drosophila
Depositor: Lipshitz, H.D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Hospital for Sick Children
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antigen is also known as adducin-like protein.
Antigen Molecular Weight: Multiple molecular weights due to multiple isoforms: the drosophila Adducin-like protein was initially reported to be 87 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: hts
Immunogen: GST-adducin fusion protein expressed in E. coli
Alternate Gene Names: add; Add; Add-hts; add-like; adducin; hu li tai shao; Ovhts; CG43443
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: aa 472-658 of isoform N4 (see additional information)
Uniprot ID: Q02645 
Immunogen Sequence: aa. 465-903 Of N4 isoform [Uniprot ID Q02645-1]
Entrez Gene ID: 37230 
Additional Characterization:
Antibody Registry ID: AB_528070 
Additional Information: 1B1 recognizes all isoforms produced by alternative splicing except ShAdd (also known as hts-PC or N32/R2 [Uniprot ID Q02645-3]). By deduction, the 1B1 epitope is located in the region of N4 [Uniprot ID Q02645-1] common to all isoforms but absent in ShAdd, and corresponds to aa 472-658 of isoform N4. 1B1 recognizes only one fragment resulting from proteolysis of Ovhts (N4): Ovhts Fus is recognized but Ovhts-RC is not.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1B1 was deposited to the DSHB by Lipshitz, H.D. (DSHB Hybridoma Product 1B1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

40 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    Epitope Map References
    All References

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