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p1B2 (anti-p120 catenin)

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$45.00 to $425.00
SKU: p1B2 (anti-p120 catenin)
View product citations for antibody p1B2 (anti-p120 catenin) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: catenin, p120
Available to For-Profits: Yes
Antigen Species: Drosophila melanogaster
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Parkhurst, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Fred Hutchinson Cancer Research Center
Antigen Molecular Weight: Predicted: 87 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant Drosophila p120 catenin/GST fusion protein
Gene: p120ctn
Alternate Antibody Name:
Alternate Gene Names: CG17484, p120, dP120ctn
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: No
Uniprot ID: Q7PLI0 
Epitope Location or Sequence:
Entrez Gene ID: 3355143 
Immunogen Sequence: Not disclosed (see "additional information).
Antibody Registry ID: AB_10660833 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: This mAb is not decribed in the "initial publication". Assuming that the immunogen used to generate this mAb was the same than the one used to generate the polyclonal antibody from [PMID 12135916], the epitope should be located in the first 222 aa of the protein.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
p1B2 (anti-p120 catenin) was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product p1B2 (anti-p120 catenin))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References
    WB References
    All References

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