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p4B2 (anti-p120 catenin)

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SKU: p4B2 (anti-p120 catenin)
View product citations for antibody p4B2 (anti-p120 catenin) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: catenin, p120
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Parkhurst, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchinson Cancer Research Center
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 87 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: p120ctn
Immunogen: Recombinant Drosophila p120 catenin/GST fusion protein
Alternate Gene Names: CG17484, p120, dP120ctn
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q7PLI0 
Immunogen Sequence: Not disclosed (see "additional information).
Entrez Gene ID: 3355143 
Additional Characterization:  
Antibody Registry ID: AB_2088073 
Additional Information: This mAb is not decribed in the "initial publication". Assuming that the immunogen used to generate this mAb was the same than the one used to generate the polyclonal antibody from [PMID 12135916], the epitope should be located in the first 222 aa of the protein.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
p4B2 (anti-p120 catenin) was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product p4B2 (anti-p120 catenin))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
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  • Initial Publication
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