p4B2 (anti-p120 catenin)

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SKU: p4B2 (anti-p120 catenin)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: p4B2 (anti-p120 catenin)
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: p120ctn
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2088073 
Uniprot ID: Q7PLI0 
Entrez Gene ID: 3355143 
Clonality: Monoclonal
Immunogen: Recombinant Drosophila p120 catenin/GST fusion protein
Immunogen Sequence: Not disclosed (see "additional information).
Myeloma Strain:
Epitope Mapped: No
Antigen Name: catenin, p120
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/9/2008
Antigen Molecular Weight: Predicted: 87 kDa
Depositor: Parkhurst, S.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Drosophila melanogaster
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: This mAb is not decribed in the "initial publication". Assuming that the immunogen used to generate this mAb was the same than the one used to generate the polyclonal antibody from [PMID 12135916], the epitope should be located in the first 222 aa of the protein.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
p4B2 (anti-p120 catenin) was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product p4B2 (anti-p120 catenin))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication
    All References

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