Ag10.2(EcR common)

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SKU: Ag10.2(EcR common)
View product citations for antibody Ag10.2(EcR common) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ecdysone receptor (EcR common)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Thummel, C. / Hogness, D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Utah
Positive Tested Species Reactivity: Amblyomma hebraeum, Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 91 kDa (EcR-A), 94 kDa (EcR-B1), 73 kDa (EcR-B2); Apparent; 105 kDa (EcR-A and EcR-B1) and 80 kDa (EcR-B2)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: EcR
Immunogen: TrpE-EcR-B1 fusion protein containing both isoform-specific and common segments of EcR-Bl (residues 68-878).
Alternate Gene Names: NR1H1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: aa 649-878
Uniprot ID: P34021 
Immunogen Sequence: Partial protein
Entrez Gene ID: 35540 
Additional Characterization:
Antibody Registry ID: AB_10683832 
Additional Information:
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ag10.2(EcR common) was deposited to the DSHB by Thummel, C. / Hogness, D. (DSHB Hybridoma Product Ag10.2(EcR common))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

20 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms.
    Hogness DS
    Cell 73.7 (1993 Jul 2): 1323-37.

    Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila.
    Segraves WA
    Development (Cambridge, England) 126.20 (1999 Oct): 4581-9.

    Ras signaling modulates activity of the ecdysone receptor EcR during cell migration in the Drosophila ovary.
    Dobens L
    Developmental dynamics : an official publication of the American Association of Anatomists 236.5 (2007 May): 1213-26.

    An ecdysone-responsive nuclear receptor regulates circadian rhythms in Drosophila.
    Sehgal A
    Nature communications 5. (2014 Dec 16): 5697.

    The ecdysone receptor signalling regulates microvilli formation in follicular epithelial cells.
    Cavaliere V
    Cellular and molecular life sciences : CMLS 73.2 (2016 Jan): 409-25.

    Ecdysteroids affect Drosophila ovarian stem cell niche formation and early germline differentiation.
    Shcherbata HR
    The EMBO journal 30.8 (2011 Apr 20): 1549-62.

    Steroid signaling promotes stem cell maintenance in the Drosophila testis.
    Matunis EL
    Developmental biology 394.1 (2014 Oct 1): 129-41.

    Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function.
    Sokol NS
    Development (Cambridge, England) 139.10 (2012 May): 1788-97.

    EcR-B1 and Usp nuclear hormone receptors regulate expression of the VM32E eggshell gene during Drosophila oogenesis.
    Cavaliere V
    Developmental biology 328.2 (2009 Apr 15): 541-51.

    WB References
    IHC References
    IP References
    Epitope Map References
    Gel Supershift References
    All References

    Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms.
    Hogness DS
    Cell 73.7 (1993 Jul 2): 1323-37.

    Ecdysone receptor expression and activity in adult Drosophila melanogaster.
    Carney GE
    Journal of insect physiology 57.7 (2011 Jul): 899-907.

    Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila.
    Segraves WA
    Development (Cambridge, England) 126.20 (1999 Oct): 4581-9.

    Ras signaling modulates activity of the ecdysone receptor EcR during cell migration in the Drosophila ovary.
    Dobens L
    Developmental dynamics : an official publication of the American Association of Anatomists 236.5 (2007 May): 1213-26.

    An ecdysone-responsive nuclear receptor regulates circadian rhythms in Drosophila.
    Sehgal A
    Nature communications 5. (2014 Dec 16): 5697.

    The ecdysone receptor signalling regulates microvilli formation in follicular epithelial cells.
    Cavaliere V
    Cellular and molecular life sciences : CMLS 73.2 (2016 Jan): 409-25.

    Ecdysteroids affect Drosophila ovarian stem cell niche formation and early germline differentiation.
    Shcherbata HR
    The EMBO journal 30.8 (2011 Apr 20): 1549-62.

    Steroid signaling promotes stem cell maintenance in the Drosophila testis.
    Matunis EL
    Developmental biology 394.1 (2014 Oct 1): 129-41.

    Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function.
    Sokol NS
    Development (Cambridge, England) 139.10 (2012 May): 1788-97.

    EcR-B1 and Usp nuclear hormone receptors regulate expression of the VM32E eggshell gene during Drosophila oogenesis.
    Cavaliere V
    Developmental biology 328.2 (2009 Apr 15): 541-51.

    DNA binding properties of the ecdysteroid receptor in the salivary gland of the female ixodid tick, Amblyomma hebraeum.
    Kaufman WR
    Insect biochemistry and molecular biology 28.12 (1998 Dec): 947-57.

    Profile of the ecdysteroid hormone and its receptor in the salivary gland of the adult female tick, Amblyomma hebraeum.
    Kaufman WR
    Insect biochemistry and molecular biology 29.1 (1999 Jan): 33-42.

    Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes.
    Verrijzer CP
    Molecular and cellular biology 30.3 (2010 Feb): 736-44.

    Ecdysone-induced receptor tyrosine phosphatase PTP52F regulates Drosophila midgut histolysis by enhancement of autophagy and apoptosis.
    Meng TC
    Molecular and cellular biology 34.9 (2014 May): 1594-606.

    PKC-mediated USP phosphorylation at Ser35 modulates 20-hydroxyecdysone signaling in Drosophila.
    Li S
    Journal of proteome research 11.12 (2012 Dec 7): 6187-96.

    Sumoylation modulates 20-hydroxyecdysone signaling by maintaining USP protein levels in Drosophila.
    Li S
    Insect biochemistry and molecular biology 54. (2014 Nov): 80-8.

    Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster.
    Demmers JA
    Proteomics 15.4 (2015 Feb): 725-38.

    Molecular chaperones activate the Drosophila ecdysone receptor, an RXR heterodimer.
    Hogness DS
    Cell 101.1 (2000 Mar 31): 67-77.

    Direct repeats bind the EcR/USP receptor and mediate ecdysteroid responses in Drosophila melanogaster.
    Lepesant JA
    Molecular and cellular biology 16.6 (1996 Jun): 2977-86.

    DNA affects ligand binding of the ecdysone receptor of Drosophila melanogaster.
    Spindler-Barth M
    Molecular and cellular endocrinology 303.1-2 (2009 May 6): 91-9.

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