1G9
Catalog Fields
Product Name/ID: 1G9
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: peb
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Entrez Gene ID: 31391
Clonality: Monoclonal
Immunogen: GST-Hindsight fusion protein expressed in E. coli
Clone:
Immunogen Sequence: aa. 824-1125
Myeloma Strain: HL-1
Epitope Mapped: Yes
Antigen Name: hindsight: zinc-finger nuclear protein hindsight
Epitope Location or Sequence: aa 824 to 1125 of Peb/Hnt
Alternate Antigen Name: pebbled
Deposit Date: 12/2/1997
Antigen Molecular Weight: Predicted: 209kDa, Apparent: ~250kDa
Depositor: Lipshitz, H.D.
Antigen Sequence:
Depositor Institution: Hospital for Sick Children
Antigen Species: Drosophila
Depositor Notes: Does not recognize grasshopper hindsight protein
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1G9 was deposited to the DSHB by Lipshitz, H.D. (DSHB Hybridoma Product 1G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
34 References
Initial Publication