1G9

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$40.00
SKU: 1G9
View product citations for antibody 1G9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: hindsight: zinc-finger nuclear protein hindsight
Hybridoma Cells Available:
Antigen Species: Drosophila
Depositor: Lipshitz, H.D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Hospital for Sick Children
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Does not recognize grasshopper hindsight protein
Antigen Molecular Weight: Predicted: 209kDa, Apparent: ~250kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: peb
Immunogen: GST-Hindsight fusion protein expressed in E. coli
Alternate Gene Names: Zinc-finger nuclear protein hindsight, CG12212, pebbledanon-4Cg; CG12212; Dmel\CG12212; EG:66A1.1; EP55; Hin; Hind; hnd; hnt; Hnt; HNT; Peb; PEB
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name: pebbled
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: aa 824 to 1125 of Peb/Hnt
Uniprot ID: o46205 
Immunogen Sequence: aa. 824-1125
Entrez Gene ID: 31391 
Additional Characterization:
Antibody Registry ID: AB_528278 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1G9 was deposited to the DSHB by Lipshitz, H.D. (DSHB Hybridoma Product 1G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

32 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication

    Development (Cambridge, England)
    Lipshitz HD
    Development (Cambridge, England) 124.11 (1997 Jun): 2129-41.

    IF References

    Development (Cambridge, England)
    Schüpbach T
    Development (Cambridge, England) 138.9 (2011 May): 1697-703.

    Current biology : CB
    Arias AM
    Current biology : CB 11.6 (2001 Mar 20): 375-85.

    IHC References

    Development (Cambridge, England)
    Lipshitz HD
    Development (Cambridge, England) 124.11 (1997 Jun): 2129-41.

    Molecular biology and evolution
    Newfeld SJ
    Molecular biology and evolution 31.9 (2014 Sep): 2309-21.

    BMC biology
    Hsu T
    BMC biology 12. (2014 Feb 14): 12.

    Development (Cambridge, England)
    Noselli S
    Development (Cambridge, England) 141.15 (2014 Aug): 3013-21.

    Development (Cambridge, England)
    Schüpbach T
    Development (Cambridge, England) 138.9 (2011 May): 1697-703.

    Current biology : CB
    Arias AM
    Current biology : CB 11.6 (2001 Mar 20): 375-85.

    Development (Cambridge, England)
    Newfeld SJ
    Development (Cambridge, England) 139.15 (2012 Aug): 2721-9.

    Developmental cell
    Tapon N
    Developmental cell 18.2 (2010 Feb 16): 300-8.

    Journal of cell science
    Schüpbach T
    Journal of cell science 125.Pt 2 (2012 Jan 15): 399-410.

    Development (Cambridge, England)
    Schüpbach T
    Development (Cambridge, England) 138.10 (2011 May): 1991-2001.

    Development (Cambridge, England)
    Perrimon N
    Development (Cambridge, England) 137.21 (2010 Nov): 3615-24.

    Developmental cell
    Deng WM
    Developmental cell 12.3 (2007 Mar): 431-42.

    Developmental cell
    Posakony JW
    Developmental cell 8.3 (2005 Mar): 413-25.

    BMC developmental biology
    Li M
    BMC developmental biology 9. (2009 Dec 1): 60.

    Nature cell biology
    Bellen HJ
    Nature cell biology 11.7 (2009 Jul): 815-24.

    BMC biology
    Cambiazo V
    BMC biology 7. (2009 Sep 22): 61.

    Development (Cambridge, England)
    Brown NH
    Development (Cambridge, England) 126.22 (1999 Nov): 5161-9.

    Mechanisms of development
    Casanova J
    Mechanisms of development 91.1-2 (2000 Mar 1): 271-8.

    Developmental cell
    Serpe M
    Developmental cell 21.2 (2011 Aug 16): 375-83.

    Development (Cambridge, England)
    Lipshitz HD
    Development (Cambridge, England) 136.6 (2009 Mar): 975-82.

    Differentiation; research in biological diversity
    Lipshitz HD
    Differentiation; research in biological diversity 86.4-5 (2013 Nov-Dec): 159-70.

    Developmental cell
    Cohen SM
    Developmental cell 2.5 (2002 May): 667-76.

    La Nouvelle presse medicale
    Dollinger A
    La Nouvelle presse medicale 4.17 (1975 Apr 26): 1292.

    Mechanisms of development
    Bogdan S
    Mechanisms of development 125.3-4 (2008 Mar-Apr): 183-95.

    Development (Cambridge, England)
    Lipshitz HD
    Development (Cambridge, England) 129.9 (2002 May): 2247-58.

    Current biology : CB
    Lipshitz HD
    Current biology : CB 11.14 (2001 Jul 24): 1098-108.

    The Journal of cell biology
    Deng WM
    The Journal of cell biology 182.5 (2008 Sep 8): 885-96.

    Development (Cambridge, England)
    Frasch M
    Development (Cambridge, England) 130.14 (2003 Jul): 3187-204.

    Development (Cambridge, England)
    Deng WM
    Development (Cambridge, England) 140.4 (2013 Feb): 897-905.

    Biology open
    Tsai CC
    Biology open 1.3 (2012 Mar 15): 182-96.

    Mechanisms of development
    Jacinto A
    Mechanisms of development 124.11-12 (2007 Nov-Dec): 884-97.

    Biochimica et biophysica acta. Molecular cell research
    Maier D
    Biochimica et biophysica acta. Molecular cell research 1866.10 (2019 Oct): 1520-1532.

    Epitope Map References

    Development (Cambridge, England)
    Lipshitz HD
    Development (Cambridge, England) 124.11 (1997 Jun): 2129-41.

    Gel Supershift References

    Differentiation; research in biological diversity
    Lipshitz HD
    Differentiation; research in biological diversity 86.4-5 (2013 Nov-Dec): 159-70.

    All References

    Development (Cambridge, England)
    Lipshitz HD
    Development (Cambridge, England) 124.11 (1997 Jun): 2129-41.

    Molecular biology and evolution
    Newfeld SJ
    Molecular biology and evolution 31.9 (2014 Sep): 2309-21.

    BMC biology
    Hsu T
    BMC biology 12. (2014 Feb 14): 12.

    Development (Cambridge, England)
    Noselli S
    Development (Cambridge, England) 141.15 (2014 Aug): 3013-21.

    Development (Cambridge, England)
    Schüpbach T
    Development (Cambridge, England) 138.9 (2011 May): 1697-703.

    Current biology : CB
    Arias AM
    Current biology : CB 11.6 (2001 Mar 20): 375-85.

    Development (Cambridge, England)
    Newfeld SJ
    Development (Cambridge, England) 139.15 (2012 Aug): 2721-9.

    Developmental cell
    Tapon N
    Developmental cell 18.2 (2010 Feb 16): 300-8.

    Journal of cell science
    Schüpbach T
    Journal of cell science 125.Pt 2 (2012 Jan 15): 399-410.

    Development (Cambridge, England)
    Schüpbach T
    Development (Cambridge, England) 138.10 (2011 May): 1991-2001.

    Development (Cambridge, England)
    Perrimon N
    Development (Cambridge, England) 137.21 (2010 Nov): 3615-24.

    Developmental cell
    Deng WM
    Developmental cell 12.3 (2007 Mar): 431-42.

    Developmental cell
    Posakony JW
    Developmental cell 8.3 (2005 Mar): 413-25.

    BMC developmental biology
    Li M
    BMC developmental biology 9. (2009 Dec 1): 60.

    Nature cell biology
    Bellen HJ
    Nature cell biology 11.7 (2009 Jul): 815-24.

    BMC biology
    Cambiazo V
    BMC biology 7. (2009 Sep 22): 61.

    Development (Cambridge, England)
    Brown NH
    Development (Cambridge, England) 126.22 (1999 Nov): 5161-9.

    Mechanisms of development
    Casanova J
    Mechanisms of development 91.1-2 (2000 Mar 1): 271-8.

    Developmental cell
    Serpe M
    Developmental cell 21.2 (2011 Aug 16): 375-83.

    Development (Cambridge, England)
    Lipshitz HD
    Development (Cambridge, England) 136.6 (2009 Mar): 975-82.

    Differentiation; research in biological diversity
    Lipshitz HD
    Differentiation; research in biological diversity 86.4-5 (2013 Nov-Dec): 159-70.

    Developmental cell
    Cohen SM
    Developmental cell 2.5 (2002 May): 667-76.

    La Nouvelle presse medicale
    Dollinger A
    La Nouvelle presse medicale 4.17 (1975 Apr 26): 1292.

    Mechanisms of development
    Bogdan S
    Mechanisms of development 125.3-4 (2008 Mar-Apr): 183-95.

    Development (Cambridge, England)
    Lipshitz HD
    Development (Cambridge, England) 129.9 (2002 May): 2247-58.

    Current biology : CB
    Lipshitz HD
    Current biology : CB 11.14 (2001 Jul 24): 1098-108.

    The Journal of cell biology
    Deng WM
    The Journal of cell biology 182.5 (2008 Sep 8): 885-96.

    Development (Cambridge, England)
    Frasch M
    Development (Cambridge, England) 130.14 (2003 Jul): 3187-204.

    Development (Cambridge, England)
    Deng WM
    Development (Cambridge, England) 140.4 (2013 Feb): 897-905.

    Biology open
    Tsai CC
    Biology open 1.3 (2012 Mar 15): 182-96.

    Mechanisms of development
    Jacinto A
    Mechanisms of development 124.11-12 (2007 Nov-Dec): 884-97.

    Biochimica et biophysica acta. Molecular cell research
    Maier D
    Biochimica et biophysica acta. Molecular cell research 1866.10 (2019 Oct): 1520-1532.

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