1G9

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$40.00
SKU: 1G9
View product citations for antibody 1G9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: hindsight: zinc-finger nuclear protein hindsight
Hybridoma Cells Available:
Antigen Species: Drosophila
Depositor: Lipshitz, H.D.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Hospital for Sick Children
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Does not recognize grasshopper hindsight protein
Antigen Molecular Weight: Predicted: 209kDa, Apparent: ~250kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: peb
Immunogen: GST-Hindsight fusion protein expressed in E. coli
Alternate Gene Names: Zinc-finger nuclear protein hindsight, CG12212, pebbledanon-4Cg; CG12212; Dmel\CG12212; EG:66A1.1; EP55; Hin; Hind; hnd; hnt; Hnt; HNT; Peb; PEB
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name: pebbled
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: aa 824 to 1125 of Peb/Hnt
Uniprot ID: o46205 
Immunogen Sequence: aa. 824-1125
Entrez Gene ID: 31391 
Additional Characterization:
Antibody Registry ID: AB_528278 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1G9 was deposited to the DSHB by Lipshitz, H.D. (DSHB Hybridoma Product 1G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

32 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication

    Control of germ-band retraction in Drosophila by the zinc-finger protein HINDSIGHT.
    Lipshitz HD
    Development (Cambridge, England) 124.11 (1997 Jun): 2129-41.

    IF References
    IHC References

    Control of germ-band retraction in Drosophila by the zinc-finger protein HINDSIGHT.
    Lipshitz HD
    Development (Cambridge, England) 124.11 (1997 Jun): 2129-41.

    New gene evolution in the bonus-TIF1-γ/TRIM33 family impacted the architecture of the vertebrate dorsal-ventral patterning network.
    Newfeld SJ
    Molecular biology and evolution 31.9 (2014 Sep): 2309-21.

    Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23.
    Hsu T
    BMC biology 12. (2014 Feb 14): 12.

    Starvation induces FoxO-dependent mitotic-to-endocycle switch pausing during Drosophila oogenesis.
    Noselli S
    Development (Cambridge, England) 141.15 (2014 Aug): 3013-21.

    Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling.
    Schüpbach T
    Development (Cambridge, England) 138.9 (2011 May): 1697-703.

    Notch signaling targets the Wingless responsiveness of a Ubx visceral mesoderm enhancer in Drosophila.
    Arias AM
    Current biology : CB 11.6 (2001 Mar 20): 375-85.

    Fat facets deubiquitylation of Medea/Smad4 modulates interpretation of a Dpp morphogen gradient.
    Newfeld SJ
    Development (Cambridge, England) 139.15 (2012 Aug): 2721-9.

    Kibra is a regulator of the Salvador/Warts/Hippo signaling network.
    Tapon N
    Developmental cell 18.2 (2010 Feb 16): 300-8.

    CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster.
    Schüpbach T
    Journal of cell science 125.Pt 2 (2012 Jan 15): 399-410.

    Regulation of somatic myosin activity by protein phosphatase 1β controls Drosophila oocyte polarization.
    Schüpbach T
    Development (Cambridge, England) 138.10 (2011 May): 1991-2001.

    Embryonic multipotent progenitors remodel the Drosophila airways during metamorphosis.
    Perrimon N
    Development (Cambridge, England) 137.21 (2010 Nov): 3615-24.

    Hindsight mediates the role of notch in suppressing hedgehog signaling and cell proliferation.
    Deng WM
    Developmental cell 12.3 (2007 Mar): 431-42.

    Genetic programs activated by proneural proteins in the developing Drosophila PNS.
    Posakony JW
    Developmental cell 8.3 (2005 Mar): 413-25.

    Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis.
    Li M
    BMC developmental biology 9. (2009 Dec 1): 60.

    The Arp2/3 complex and WASp are required for apical trafficking of Delta into microvilli during cell fate specification of sensory organ precursors.
    Bellen HJ
    Nature cell biology 11.7 (2009 Jul): 815-24.

    Genes encoding novel secreted and transmembrane proteins are temporally and spatially regulated during Drosophila melanogaster embryogenesis.
    Cambiazo V
    BMC biology 7. (2009 Sep 22): 61.

    Migration of the Drosophila primordial midgut cells requires coordination of diverse PS integrin functions.
    Brown NH
    Development (Cambridge, England) 126.22 (1999 Nov): 5161-9.

    Cross-regulatory interactions among tracheal genes support a co-operative model for the induction of tracheal fates in the Drosophila embryo.
    Casanova J
    Mechanisms of development 91.1-2 (2000 Mar 1): 271-8.

    Shaping BMP morphogen gradients through enzyme-substrate interactions.
    Serpe M
    Developmental cell 21.2 (2011 Aug 16): 375-83.

    Hindsight modulates Delta expression during Drosophila cone cell induction.
    Lipshitz HD
    Development (Cambridge, England) 136.6 (2009 Mar): 975-82.

    Drosophila Hindsight and mammalian RREB-1 are evolutionarily conserved DNA-binding transcriptional attenuators.
    Lipshitz HD
    Differentiation; research in biological diversity 86.4-5 (2013 Nov-Dec): 159-70.

    HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient.
    Cohen SM
    Developmental cell 2.5 (2002 May): 667-76.

    [Letter: Idiopathic cyclic edema syndrome].
    Dollinger A
    La Nouvelle presse medicale 4.17 (1975 Apr 26): 1292.

    Abi induces ectopic sensory organ formation by stimulating EGFR signaling.
    Bogdan S
    Mechanisms of development 125.3-4 (2008 Mar-Apr): 183-95.

    Control of photoreceptor cell morphology, planar polarity and epithelial integrity during Drosophila eye development.
    Lipshitz HD
    Development (Cambridge, England) 129.9 (2002 May): 2247-58.

    Downregulation of Jun kinase signaling in the amnioserosa is essential for dorsal closure of the Drosophila embryo.
    Lipshitz HD
    Current biology : CB 11.14 (2001 Jul 24): 1098-108.

    Regulation of the endocycle/gene amplification switch by Notch and ecdysone signaling.
    Deng WM
    The Journal of cell biology 182.5 (2008 Sep 8): 885-96.

    The T-box-encoding Dorsocross genes function in amnioserosa development and the patterning of the dorsolateral germ band downstream of Dpp.
    Frasch M
    Development (Cambridge, England) 130.14 (2003 Jul): 3187-204.

    The microRNA miR-7 regulates Tramtrack69 in a developmental switch in Drosophila follicle cells.
    Deng WM
    Development (Cambridge, England) 140.4 (2013 Feb): 897-905.

    The transcriptional corepressor SMRTER influences both Notch and ecdysone signaling during Drosophila development.
    Tsai CC
    Biology open 1.3 (2012 Mar 15): 182-96.

    Dpp signalling orchestrates dorsal closure by regulating cell shape changes both in the amnioserosa and in the epidermis.
    Jacinto A
    Mechanisms of development 124.11-12 (2007 Nov-Dec): 884-97.

    Nucleo-cytoplasmic shuttling of Drosophila Hairless/Su(H) heterodimer as a means of regulating Notch dependent transcription.
    Maier D
    Biochimica et biophysica acta. Molecular cell research 1866.10 (2019 Oct): 1520-1532.

    Epitope Map References

    Control of germ-band retraction in Drosophila by the zinc-finger protein HINDSIGHT.
    Lipshitz HD
    Development (Cambridge, England) 124.11 (1997 Jun): 2129-41.

    Gel Supershift References

    Drosophila Hindsight and mammalian RREB-1 are evolutionarily conserved DNA-binding transcriptional attenuators.
    Lipshitz HD
    Differentiation; research in biological diversity 86.4-5 (2013 Nov-Dec): 159-70.

    All References

    Control of germ-band retraction in Drosophila by the zinc-finger protein HINDSIGHT.
    Lipshitz HD
    Development (Cambridge, England) 124.11 (1997 Jun): 2129-41.

    New gene evolution in the bonus-TIF1-γ/TRIM33 family impacted the architecture of the vertebrate dorsal-ventral patterning network.
    Newfeld SJ
    Molecular biology and evolution 31.9 (2014 Sep): 2309-21.

    Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23.
    Hsu T
    BMC biology 12. (2014 Feb 14): 12.

    Starvation induces FoxO-dependent mitotic-to-endocycle switch pausing during Drosophila oogenesis.
    Noselli S
    Development (Cambridge, England) 141.15 (2014 Aug): 3013-21.

    Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling.
    Schüpbach T
    Development (Cambridge, England) 138.9 (2011 May): 1697-703.

    Notch signaling targets the Wingless responsiveness of a Ubx visceral mesoderm enhancer in Drosophila.
    Arias AM
    Current biology : CB 11.6 (2001 Mar 20): 375-85.

    Fat facets deubiquitylation of Medea/Smad4 modulates interpretation of a Dpp morphogen gradient.
    Newfeld SJ
    Development (Cambridge, England) 139.15 (2012 Aug): 2721-9.

    Kibra is a regulator of the Salvador/Warts/Hippo signaling network.
    Tapon N
    Developmental cell 18.2 (2010 Feb 16): 300-8.

    CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster.
    Schüpbach T
    Journal of cell science 125.Pt 2 (2012 Jan 15): 399-410.

    Regulation of somatic myosin activity by protein phosphatase 1β controls Drosophila oocyte polarization.
    Schüpbach T
    Development (Cambridge, England) 138.10 (2011 May): 1991-2001.

    Embryonic multipotent progenitors remodel the Drosophila airways during metamorphosis.
    Perrimon N
    Development (Cambridge, England) 137.21 (2010 Nov): 3615-24.

    Hindsight mediates the role of notch in suppressing hedgehog signaling and cell proliferation.
    Deng WM
    Developmental cell 12.3 (2007 Mar): 431-42.

    Genetic programs activated by proneural proteins in the developing Drosophila PNS.
    Posakony JW
    Developmental cell 8.3 (2005 Mar): 413-25.

    Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis.
    Li M
    BMC developmental biology 9. (2009 Dec 1): 60.

    The Arp2/3 complex and WASp are required for apical trafficking of Delta into microvilli during cell fate specification of sensory organ precursors.
    Bellen HJ
    Nature cell biology 11.7 (2009 Jul): 815-24.

    Genes encoding novel secreted and transmembrane proteins are temporally and spatially regulated during Drosophila melanogaster embryogenesis.
    Cambiazo V
    BMC biology 7. (2009 Sep 22): 61.

    Migration of the Drosophila primordial midgut cells requires coordination of diverse PS integrin functions.
    Brown NH
    Development (Cambridge, England) 126.22 (1999 Nov): 5161-9.

    Cross-regulatory interactions among tracheal genes support a co-operative model for the induction of tracheal fates in the Drosophila embryo.
    Casanova J
    Mechanisms of development 91.1-2 (2000 Mar 1): 271-8.

    Shaping BMP morphogen gradients through enzyme-substrate interactions.
    Serpe M
    Developmental cell 21.2 (2011 Aug 16): 375-83.

    Hindsight modulates Delta expression during Drosophila cone cell induction.
    Lipshitz HD
    Development (Cambridge, England) 136.6 (2009 Mar): 975-82.

    Drosophila Hindsight and mammalian RREB-1 are evolutionarily conserved DNA-binding transcriptional attenuators.
    Lipshitz HD
    Differentiation; research in biological diversity 86.4-5 (2013 Nov-Dec): 159-70.

    HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient.
    Cohen SM
    Developmental cell 2.5 (2002 May): 667-76.

    [Letter: Idiopathic cyclic edema syndrome].
    Dollinger A
    La Nouvelle presse medicale 4.17 (1975 Apr 26): 1292.

    Abi induces ectopic sensory organ formation by stimulating EGFR signaling.
    Bogdan S
    Mechanisms of development 125.3-4 (2008 Mar-Apr): 183-95.

    Control of photoreceptor cell morphology, planar polarity and epithelial integrity during Drosophila eye development.
    Lipshitz HD
    Development (Cambridge, England) 129.9 (2002 May): 2247-58.

    Downregulation of Jun kinase signaling in the amnioserosa is essential for dorsal closure of the Drosophila embryo.
    Lipshitz HD
    Current biology : CB 11.14 (2001 Jul 24): 1098-108.

    Regulation of the endocycle/gene amplification switch by Notch and ecdysone signaling.
    Deng WM
    The Journal of cell biology 182.5 (2008 Sep 8): 885-96.

    The T-box-encoding Dorsocross genes function in amnioserosa development and the patterning of the dorsolateral germ band downstream of Dpp.
    Frasch M
    Development (Cambridge, England) 130.14 (2003 Jul): 3187-204.

    The microRNA miR-7 regulates Tramtrack69 in a developmental switch in Drosophila follicle cells.
    Deng WM
    Development (Cambridge, England) 140.4 (2013 Feb): 897-905.

    The transcriptional corepressor SMRTER influences both Notch and ecdysone signaling during Drosophila development.
    Tsai CC
    Biology open 1.3 (2012 Mar 15): 182-96.

    Dpp signalling orchestrates dorsal closure by regulating cell shape changes both in the amnioserosa and in the epidermis.
    Jacinto A
    Mechanisms of development 124.11-12 (2007 Nov-Dec): 884-97.

    Nucleo-cytoplasmic shuttling of Drosophila Hairless/Su(H) heterodimer as a means of regulating Notch dependent transcription.
    Maier D
    Biochimica et biophysica acta. Molecular cell research 1866.10 (2019 Oct): 1520-1532.

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