3.1C12

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SKU: 3.1C12

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 3.1C12
Available to For-Profits: Yes
Alternate Antibody Name: 1C12
Gene Symbol: Cntn2
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2229763 
Uniprot ID: P22063 
RRID:  
Entrez Gene ID: 25356 
Clonality: Monoclonal
Immunogen: Rat (E14-E15) spinal cord membranes
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Contactin-2, neuronal marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/15/1992
Antigen Molecular Weight: Predicted: 104 kDa; Apparent: 120 kDa (nonreducing conditions), 135 kDa (reducing conditions)
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Antigen Sequence:
Depositor Institution: Columbia University
Antigen Species: rat
Depositor Notes: Stains motoneurons, commissural neurons and central afferents of the DRG. Works on western blots of purified TAG-1.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Rat
Additional Information: This antibody may work with some mouse strains.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3.1C12 was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 3.1C12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • All References
  • Initial Publication
    IF References

    Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons.
    Jessell TM
    Neuron 1.2 (1988 Apr): 105-16.

    Differential modulation of the juxtaparanodal complex in Multiple Sclerosis.
    Karagogeos D
    Molecular and cellular neurosciences 67. (2015 Jul): 93-103.

    Neuronal and glial expression of the adhesion molecule TAG-1 is regulated after peripheral nerve lesion or central neurodegeneration of adult nervous system.
    Nothias F
    The European journal of neuroscience 21.5 (2005 Mar): 1169-80.

    Early stages in the development of spinal motor neurons.
    Chiu AY
    The Journal of comparative neurology 320.3 (1992 Jun 15): 291-303.

    The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of tag-1 homozygous mutants in the CNS.
    Karagogeos D
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.42 (2010 Oct 20): 13943-54.

    Select Septate Junction Proteins Direct ROS-Mediated Paracrine Regulation of Drosophila Cardiac Function.
    Wang W
    Cell reports 28.6 (2019 Aug 6): 1455-1470.e4.

    WB References
    IHC References
    IP References
    FFPE References
    All References

    Expression of the cell adhesion proteins BEN/SC1/DM-GRASP and TAG-1 defines early steps of axonogenesis in the human spinal cord.
    Pourquié O
    The Journal of comparative neurology 379.3 (1997 Mar 17): 415-27.

    The gene for the axonal cell adhesion molecule TAX-1 is amplified and aberrantly expressed in malignant gliomas.
    Hanash SM
    Cancer research 61.5 (2001 Mar 1): 2162-8.

    Neuronal and glial expression of the adhesion molecule TAG-1 is regulated after peripheral nerve lesion or central neurodegeneration of adult nervous system.
    Nothias F
    The European journal of neuroscience 21.5 (2005 Mar): 1169-80.

    Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons.
    Jessell TM
    Neuron 1.2 (1988 Apr): 105-16.

    Differential modulation of the juxtaparanodal complex in Multiple Sclerosis.
    Karagogeos D
    Molecular and cellular neurosciences 67. (2015 Jul): 93-103.

    Early stages in the development of spinal motor neurons.
    Chiu AY
    The Journal of comparative neurology 320.3 (1992 Jun 15): 291-303.

    The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of tag-1 homozygous mutants in the CNS.
    Karagogeos D
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.42 (2010 Oct 20): 13943-54.

    Select Septate Junction Proteins Direct ROS-Mediated Paracrine Regulation of Drosophila Cardiac Function.
    Wang W
    Cell reports 28.6 (2019 Aug 6): 1455-1470.e4.

    Mutation of the BiP/GRP78 gene causes axon outgrowth and fasciculation defects in the thalamocortical connections of the mammalian forebrain.
    Dwyer ND
    The Journal of comparative neurology 521.3 (2013 Feb 15): 677-96.

    A TAG1-APP signalling pathway through Fe65 negatively modulates neurogenesis.
    Xiao ZC
    Nature cell biology 10.3 (2008 Mar): 283-94.

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