34B3 anti-Fasciclin II

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$40.00
SKU: 34B3 anti-Fasciclin II
View product citations for antibody 34B3 anti-Fasciclin II on CiteAb

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Available: 86

DSHB Data Sheet

Catalog Fields

Antigen: fasciclin II (Drosophila, all isoforms)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Extracellular cell binding.
Antigen Molecular Weight: Predicted: three main isoforms of 97, 90, and 86 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Fas2
Immunogen: recombinant fusion protein, trpE fused to two different, nonoverlapping fragments of Drosophila fasII cDNA: a 1227 bp XhoI-HindIII fragment encopassing Ig domains 2-5 and a 496 bp SalI-ClaI fragment including fibronectin domain 2.
Alternate Gene Names: FasII; CG3665
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63.Ag8.653
Epitope Location or Sequence: extracellular domain of all four isoforms
Uniprot ID: P34082 
Immunogen Sequence: Partial protein
Entrez Gene ID: 31364 
Additional Characterization:  
Antibody Registry ID: AB_528236 
Additional Information: The initial publication and the immunogen information are only putative and may or may not be correct. The depositor didn't specify any initial publication and didn't indicate the immunogen used. The mAb described in [PMID 1913818] has the generic name: anti-fasciclin II Mab.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
34B3 anti-Fasciclin II was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 34B3 anti-Fasciclin II)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    A modular toolset for recombination transgenesis and neurogenetic analysis of Drosophila.
    McCabe BD
    PloS one 7.7 (2012): e42102.

    Gain-of-function screen identifies a role of the Src64 oncogene in Drosophila mushroom body development.
    Dura JM
    Journal of neurobiology 57.3 (2003 Dec): 291-302.

    The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules.
    Dow JA
    Nature communications 7. (2016 Apr 13): 11266.

    Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response.
    Chien CT
    PLoS genetics 12.10 (2016 Oct): e1006362.

    Glia-derived temporal signals orchestrate neurogenesis in the <i>Drosophila</i> mushroom body.
    Ho MS
    Proceedings of the National Academy of Sciences of the United States of America 118.23 (2021 Jun 8): .

    WB References

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules.
    Dow JA
    Nature communications 7. (2016 Apr 13): 11266.

    IHC References
    Epitope Map References

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    All References

    Genetic analysis of growth cone guidance in Drosophila: fasciclin II functions as a neuronal recognition molecule.
    Goodman CS
    Cell 67.1 (1991 Oct 4): 45-57.

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    A modular toolset for recombination transgenesis and neurogenetic analysis of Drosophila.
    McCabe BD
    PloS one 7.7 (2012): e42102.

    Gain-of-function screen identifies a role of the Src64 oncogene in Drosophila mushroom body development.
    Dura JM
    Journal of neurobiology 57.3 (2003 Dec): 291-302.

    The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules.
    Dow JA
    Nature communications 7. (2016 Apr 13): 11266.

    Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response.
    Chien CT
    PLoS genetics 12.10 (2016 Oct): e1006362.

    Glia-derived temporal signals orchestrate neurogenesis in the <i>Drosophila</i> mushroom body.
    Ho MS
    Proceedings of the National Academy of Sciences of the United States of America 118.23 (2021 Jun 8): .

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