3E8-3D3

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$40.00
SKU: 3E8-3D3
View product citations for antibody 3E8-3D3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Saide, J.D.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Boston University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 224.5 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Mhc
Immunogen: Purified myofibril Z-disks
Alternate Gene Names: Dmel_CG17927
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: S2 region of MHC
Uniprot ID: P05661 
Immunogen Sequence: Full length
Entrez Gene ID: 35007 
Additional Characterization:
Antibody Registry ID: AB_2721944 
Additional Information: FBgn0264695 RRID:AB_2721944
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3E8-3D3 was deposited to the DSHB by Saide, J.D. (DSHB Hybridoma Product 3E8-3D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    Epitope Map References

    Flightin is essential for thick filament assembly and sarcomere stability in Drosophila flight muscles.
    Vigoreaux JO
    The Journal of cell biology 151.7 (2000 Dec 25): 1483-500.

    All References

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