3E8-3D3

(0) No Reviews yet
$40.00
SKU: 3E8-3D3
View product citations for antibody 3E8-3D3 on CiteAb

In Stock

Available: 124

DSHB Data Sheet

Catalog Fields

Antigen: Myosin
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Saide, J.D.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Boston University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 224.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Mhc
Immunogen: Purified myofibril Z-disks
Alternate Gene Names: Dmel_CG17927
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: S2 region of MHC
Uniprot ID: P05661 
Immunogen Sequence: Full length
Entrez Gene ID: 35007 
Additional Characterization:  
Antibody Registry ID: AB_2721944 
Additional Information: FBgn0264695 RRID:AB_2721944
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3E8-3D3 was deposited to the DSHB by Saide, J.D. (DSHB Hybridoma Product 3E8-3D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    Epitope Map References

    Flightin is essential for thick filament assembly and sarcomere stability in Drosophila flight muscles.
    Vigoreaux JO
    The Journal of cell biology 151.7 (2000 Dec 25): 1483-500.

    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review