3H1D8F5 anti-gigas

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SKU: 3H1D8F5 anti-gigas
View product citations for antibody 3H1D8F5 anti-gigas on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Gigas/homolog of human TSC2
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California at Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The antigen Gigas is the Drosophila homolog of tuberous sclerosis gene product-2.
Antigen Molecular Weight: 204 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: gig
Immunogen: GST-Drosophila TSC2 fusion protein
Alternate Gene Names: gigas, Dmel_CG6975
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3XAg8.653
Epitope Location or Sequence:
Uniprot ID: Q9U9B0 
Immunogen Sequence: a.a. 1203-1531
Entrez Gene ID: 40201 
Additional Characterization:  
Antibody Registry ID: AB_528267 
Additional Information: RRID: AB_528267. FBgn0005198.
Recommended Applications: Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H1D8F5 anti-gigas was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 3H1D8F5 anti-gigas)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • All References
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