3H1D8F5 anti-gigas

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SKU: 3H1D8F5 anti-gigas
View product citations for antibody 3H1D8F5 anti-gigas on CiteAb

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Available: 35

DSHB Data Sheet

Catalog Fields

Antigen: Gigas/homolog of human TSC2
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California at Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The antigen Gigas is the Drosophila homolog of tuberous sclerosis gene product-2.
Antigen Molecular Weight: 204 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: gig
Immunogen: GST-Drosophila TSC2 fusion protein
Alternate Gene Names: gigas, Dmel_CG6975
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3XAg8.653
Epitope Location or Sequence:
Uniprot ID: Q9U9B0 
Immunogen Sequence: a.a. 1203-1531
Entrez Gene ID: 40201 
Additional Characterization:
Antibody Registry ID: AB_528267 
Additional Information: RRID: AB_528267. FBgn0005198.
Recommended Applications: Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H1D8F5 anti-gigas was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 3H1D8F5 anti-gigas)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IP References
  • All References
  • Initial Publication
    IF References
    IP References

    Akt regulates growth by directly phosphorylating Tsc2.
    Xu T
    Nature cell biology 4.9 (2002 Sep): 658-65.

    All References

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