4C9H4 anti-peanut

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SKU: 4C9H4 anti-peanut

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 4C9H4 anti-peanut
Available to For-Profits: Yes
Alternate Antibody Name: 4C9
Gene Name: pnut
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528429 
Uniprot ID: P40797 
RRID:  
Entrez Gene ID: 35801 
Clonality: Monoclonal
Immunogen: GST-fusion protein containing the amino-terminal 116 aa of the peanut protein.
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: Yes
Antigen Name: peanut gene protein product
Epitope Location or Sequence: amino-terminal 116 aa
Alternate Antigen Name:
Deposit Date: 8/31/1994
Antigen Molecular Weight: Predicted: 60 kDa; Apparent: 60 kDa
Depositor: Rubin, G.M.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: 4C9H4 is an excellent marker for cytokinesis, and labels the cleavage furrow of dividing embryonic and imaginal disc cells. It recognizes the product of the peanut gene, which encodes a protein similar to the bud neck filament proteins of S. cerevisiae (CDC3, CDC10, CDC11 and CDC12).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Peanut is a septin
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4C9H4 anti-peanut was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 4C9H4 anti-peanut)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    The Drosophila peanut gene is required for cytokinesis and encodes a protein similar to yeast putative bud neck filament proteins.
    Rubin GM
    Cell 77.3 (1994 May 6): 371-9.

    Drosophila polo kinase is required for cytokinesis.
    Glover DM
    The Journal of cell biology 143.3 (1998 Nov 2): 659-71.

    Evidence for functional differentiation among Drosophila septins in cytokinesis and cellularization.
    Peifer M
    Molecular biology of the cell 11.9 (2000 Sep): 3123-35.

    Localization and possible functions of Drosophila septins.
    Pringle JR
    Molecular biology of the cell 6.12 (1995 Dec): 1843-59.

    D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut.
    Johansen J
    Journal of neurobiology 64.2 (2005 Aug): 157-69.

    Identification of septin-interacting proteins and characterization of the Smt3/SUMO-conjugation system in Drosophila.
    Peifer M
    Journal of cell science 115.Pt 6 (2002 Mar 15): 1259-71.

    A requirement for the Abnormal Spindle protein to organise microtubules of the central spindle for cytokinesis in Drosophila.
    Avides Mdo C
    Journal of cell science 115.Pt 5 (2002 Mar 1): 913-22.

    The novel zinc finger protein dASCIZ regulates mitosis in Drosophila via an essential role in dynein light-chain expression.
    Quinn LM
    Genetics 196.2 (2014 Feb): 443-53.

    Partial Functional Diversification of Drosophila melanogaster Septin Genes Sep2 and Sep5.
    Clark DV
    G3 (Bethesda, Md.) 6.7 (2016 Jul 7): 1947-57.

    Stabilization of the actomyosin ring enables spermatocyte cytokinesis in Drosophila.
    Brill JA
    Molecular biology of the cell 21.9 (2010 May 1): 1482-93.

    Drak Is Required for Actomyosin Organization During Drosophila Cellularization.
    Thomas JH
    G3 (Bethesda, Md.) 6.4 (2016 Apr 7): 819-28.

    Syndapin promotes pseudocleavage furrow formation by actin organization in the syncytial Drosophila embryo.
    Rikhy R
    Molecular biology of the cell 27.13 (2016 Jul 1): 2064-79.

    WB References
    IHC References
    IP References

    Localization and possible functions of Drosophila septins.
    Pringle JR
    Molecular biology of the cell 6.12 (1995 Dec): 1843-59.

    D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut.
    Johansen J
    Journal of neurobiology 64.2 (2005 Aug): 157-69.

    Epitope Map References
    All References

    Atypical laminin spots and pull-generated microtubule-actin projections mediate Drosophila wing adhesion.
    Pastor-Pareja JC
    Cell reports 36.10 (2021 Sep 7): 109667.

    The Drosophila peanut gene is required for cytokinesis and encodes a protein similar to yeast putative bud neck filament proteins.
    Rubin GM
    Cell 77.3 (1994 May 6): 371-9.

    Drosophila polo kinase is required for cytokinesis.
    Glover DM
    The Journal of cell biology 143.3 (1998 Nov 2): 659-71.

    Evidence for functional differentiation among Drosophila septins in cytokinesis and cellularization.
    Peifer M
    Molecular biology of the cell 11.9 (2000 Sep): 3123-35.

    Localization and possible functions of Drosophila septins.
    Pringle JR
    Molecular biology of the cell 6.12 (1995 Dec): 1843-59.

    D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut.
    Johansen J
    Journal of neurobiology 64.2 (2005 Aug): 157-69.

    Identification of septin-interacting proteins and characterization of the Smt3/SUMO-conjugation system in Drosophila.
    Peifer M
    Journal of cell science 115.Pt 6 (2002 Mar 15): 1259-71.

    A requirement for the Abnormal Spindle protein to organise microtubules of the central spindle for cytokinesis in Drosophila.
    Avides Mdo C
    Journal of cell science 115.Pt 5 (2002 Mar 1): 913-22.

    The novel zinc finger protein dASCIZ regulates mitosis in Drosophila via an essential role in dynein light-chain expression.
    Quinn LM
    Genetics 196.2 (2014 Feb): 443-53.

    Partial Functional Diversification of Drosophila melanogaster Septin Genes Sep2 and Sep5.
    Clark DV
    G3 (Bethesda, Md.) 6.7 (2016 Jul 7): 1947-57.

    Stabilization of the actomyosin ring enables spermatocyte cytokinesis in Drosophila.
    Brill JA
    Molecular biology of the cell 21.9 (2010 May 1): 1482-93.

    Drak Is Required for Actomyosin Organization During Drosophila Cellularization.
    Thomas JH
    G3 (Bethesda, Md.) 6.4 (2016 Apr 7): 819-28.

    Syndapin promotes pseudocleavage furrow formation by actin organization in the syncytial Drosophila embryo.
    Rikhy R
    Molecular biology of the cell 27.13 (2016 Jul 1): 2064-79.

    A purified Drosophila septin complex forms filaments and exhibits GTPase activity.
    Mitchison TJ
    The Journal of cell biology 133.3 (1996 May): 605-16.

    Dual function of Src in the maintenance of adherens junctions during tracheal epithelial morphogenesis.
    Hayashi S
    Development (Cambridge, England) 135.7 (2008 Apr): 1355-64.

    Journal of the American Physical Therapy Association annual conference-1979.

    Physical therapy 59.5 (1979 May): 546-81.

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