5B8

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$40.00
SKU: 5B8
View product citations for antibody 5B8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Neural Cell Adhesion molecular 1; NCAM
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Columbia University
Positive Tested Species Reactivity: Canine, Mouse, Rat
Depositors Notes: This antibody has also been used for isolation of neuronal and glial precursors by immunopanning (PMID: 16631155). 5B8 also recognizes human NCAM in IMR-32 cells (PMID: 12023285).
Antigen Molecular Weight: 140 kDa /180 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Ncam1
Immunogen: Embryonic spinal cord membranes
Alternate Gene Names: 94.6 kDa
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: Cytoplasmic domain
Uniprot ID: P13596 
Immunogen Sequence: Full length protein
Entrez Gene ID: 24586 
Additional Characterization:
Antibody Registry ID: AB_528393 
Additional Information: RRID: AB_528393
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5B8 was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 5B8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

29 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Afadin orients cell division to position the tubule lumen in developing renal tubules.
    Marciano DK
    Development (Cambridge, England) 144.19 (2017 Oct 1): 3511-3520.

    Functional Human Podocytes Generated in Organoids from Amniotic Fluid Stem Cells.
    Remuzzi G
    Journal of the American Society of Nephrology : JASN 27.5 (2016 May): 1400-11.

    Functional Human Podocytes Generated in Organoids from Amniotic Fluid Stem Cells.
    Remuzzi G
    Journal of the American Society of Nephrology : JASN 27.5 (2016 May): 1400-11.

    Direct reprogramming of human bone marrow stromal cells into functional renal cells using cell-free extracts.
    Benigni A
    Stem cell reports 4.4 (2015 Apr 14): 685-98.

    Renal primordia activate kidney regenerative events in a rat model of progressive renal disease.
    Morigi M
    PloS one 10.3 (2015): e0120235.

    The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
    Schachner M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.42 (2013 Oct 16): 16828-45.

    A novel model of surgical injury in adult rat kidney: a "pouch model".
    Singh AK
    Scientific reports 3. (2013 Oct 8): 2890.

    Inhibiting angiotensin-converting enzyme promotes renal repair by limiting progenitor cell proliferation and restoring the glomerular architecture.
    Remuzzi G
    The American journal of pathology 179.2 (2011 Aug): 628-38.

    A yeast display immunoprecipitation method for efficient isolation and characterization of antigens.
    Shusta EV
    Journal of immunological methods 341.1-2 (2009 Feb 28): 117-26.

    Patterned neuropathologic events occurring in hyh congenital hydrocephalic mutant mice.
    Pérez-Fígares JM
    Journal of neuropathology and experimental neurology 66.12 (2007 Dec): 1082-92.

    Math1 controls cerebellar granule cell differentiation by regulating multiple components of the Notch signaling pathway.
    Ben-Arie N
    Development (Cambridge, England) 131.4 (2004 Feb): 903-13.

    Neural cell adhesion molecule (NCAM) association with PKCbeta2 via betaI spectrin is implicated in NCAM-mediated neurite outgrowth.
    Schachner M
    The Journal of cell biology 161.3 (2003 May 12): 625-39.

    Evidence that Sry is expressed in pre-Sertoli cells and Sertoli and granulosa cells have a common precursor.
    Eicher EM
    Developmental biology 240.1 (2001 Dec 1): 92-107.

    Interactions of the chondroitin sulfate proteoglycan phosphacan, the extracellular domain of a receptor-type protein tyrosine phosphatase, with neurons, glia, and neural cell adhesion molecules.
    Margolis RU
    The Journal of cell biology 127.6 Pt 1 (1994 Dec): 1703-15.

    WB References

    The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
    Schachner M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.42 (2013 Oct 16): 16828-45.

    NCAM-induced neurite outgrowth depends on binding of calmodulin to NCAM and on nuclear import of NCAM and fak fragments.
    Schachner M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.32 (2010 Aug 11): 10784-98.

    A yeast display immunoprecipitation method for efficient isolation and characterization of antigens.
    Shusta EV
    Journal of immunological methods 341.1-2 (2009 Feb 28): 117-26.

    Inhibition of bFGF-receptor type 2 increases kidney damage and suppresses nephrogenic protein expression after ischemic acute renal failure.
    Vio CP
    American journal of physiology. Regulatory, integrative and comparative physiology 294.3 (2008 Mar): R819-28.

    Neural cell adhesion molecule (NCAM) association with PKCbeta2 via betaI spectrin is implicated in NCAM-mediated neurite outgrowth.
    Schachner M
    The Journal of cell biology 161.3 (2003 May 12): 625-39.

    Dynamic change of neural cell adhesion molecule polysialylation on human neuroblastoma (IMR-32) and rat pheochromocytoma (PC-12) cells during growth and differentiation.
    Inoue Y
    The Journal of biological chemistry 277.31 (2002 Aug 2): 28200-11.

    Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons.
    Jessell TM
    Neuron 1.2 (1988 Apr): 105-16.

    IHC References

    Sall1 in renal stromal progenitors non-cell autonomously restricts the excessive expansion of nephron progenitors.
    Nishinakamura R
    Scientific reports 5. (2015 Oct 29): 15676.

    Direct reprogramming of human bone marrow stromal cells into functional renal cells using cell-free extracts.
    Benigni A
    Stem cell reports 4.4 (2015 Apr 14): 685-98.

    A novel model of surgical injury in adult rat kidney: a "pouch model".
    Singh AK
    Scientific reports 3. (2013 Oct 8): 2890.

    De novo lumen formation and elongation in the developing nephron: a central role for afadin in apical polarity.
    Marciano DK
    Development (Cambridge, England) 140.8 (2013 Apr): 1774-84.

    Disorganized olfactory bulb lamination in mice deficient for transcription factor AP-2epsilon.
    Williams T
    Molecular and cellular neurosciences 42.3 (2009 Nov): 161-71.

    The Na(+)/Ca(2+) exchanger NCKX4 governs termination and adaptation of the mammalian olfactory response.
    Zhao H
    Nature neuroscience 15.1 (2011 Nov 6): 131-7.

    A targeted constitutive mutation in the APC tumor suppressor gene underlies mammary but not intestinal tumorigenesis.
    Fodde R
    PLoS genetics 5.7 (2009 Jul): e1000547.

    Inhibition of bFGF-receptor type 2 increases kidney damage and suppresses nephrogenic protein expression after ischemic acute renal failure.
    Vio CP
    American journal of physiology. Regulatory, integrative and comparative physiology 294.3 (2008 Mar): R819-28.

    Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts.
    Schachner M
    The Journal of cell biology 159.4 (2002 Nov 25): 649-61.

    Evidence that Sry is expressed in pre-Sertoli cells and Sertoli and granulosa cells have a common precursor.
    Eicher EM
    Developmental biology 240.1 (2001 Dec 1): 92-107.

    p75NTR and Trk receptors are expressed in reciprocal patterns in a wide variety of non-neural tissues during rat embryonic development, indicating independent receptor functions.
    Vazquez L
    The Journal of comparative neurology 391.4 (1998 Feb 22): 407-28.

    An immunochemical, ultrastructural, and developmental characterization of the horizontal basal cells of rat olfactory epithelium.
    Schwob JE
    The Journal of comparative neurology 363.1 (1995 Dec 4): 129-46.

    Interactions of the chondroitin sulfate proteoglycan phosphacan, the extracellular domain of a receptor-type protein tyrosine phosphatase, with neurons, glia, and neural cell adhesion molecules.
    Margolis RU
    The Journal of cell biology 127.6 Pt 1 (1994 Dec): 1703-15.

    Dynamic expression patterns of tenascin, proteoglycans, and cell adhesion molecules during human hair follicle morphogenesis.
    Holbrook KA
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 141-55.

    Spatiotemporal patterns of expression of NGF and the low-affinity NGF receptor in rat embryos suggest functional roles in tissue morphogenesis and myogenesis.
    Bothwell M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 12.3 (1992 Mar): 930-45.

    Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons.
    Jessell TM
    Neuron 1.2 (1988 Apr): 105-16.

    IP References

    The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
    Schachner M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.42 (2013 Oct 16): 16828-45.

    NCAM-induced neurite outgrowth depends on binding of calmodulin to NCAM and on nuclear import of NCAM and fak fragments.
    Schachner M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.32 (2010 Aug 11): 10784-98.

    ELISA References

    HMGB4 is expressed by neuronal cells and affects the expression of genes involved in neural differentiation.
    Rauvala H
    Scientific reports 6. (2016 Sep 9): 32960.

    NCAM-induced neurite outgrowth depends on binding of calmodulin to NCAM and on nuclear import of NCAM and fak fragments.
    Schachner M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.32 (2010 Aug 11): 10784-98.

    FFPE References
    Epitope Map References
    All References

    Sall1 in renal stromal progenitors non-cell autonomously restricts the excessive expansion of nephron progenitors.
    Nishinakamura R
    Scientific reports 5. (2015 Oct 29): 15676.

    Direct reprogramming of human bone marrow stromal cells into functional renal cells using cell-free extracts.
    Benigni A
    Stem cell reports 4.4 (2015 Apr 14): 685-98.

    A novel model of surgical injury in adult rat kidney: a "pouch model".
    Singh AK
    Scientific reports 3. (2013 Oct 8): 2890.

    De novo lumen formation and elongation in the developing nephron: a central role for afadin in apical polarity.
    Marciano DK
    Development (Cambridge, England) 140.8 (2013 Apr): 1774-84.

    Disorganized olfactory bulb lamination in mice deficient for transcription factor AP-2epsilon.
    Williams T
    Molecular and cellular neurosciences 42.3 (2009 Nov): 161-71.

    The Na(+)/Ca(2+) exchanger NCKX4 governs termination and adaptation of the mammalian olfactory response.
    Zhao H
    Nature neuroscience 15.1 (2011 Nov 6): 131-7.

    A targeted constitutive mutation in the APC tumor suppressor gene underlies mammary but not intestinal tumorigenesis.
    Fodde R
    PLoS genetics 5.7 (2009 Jul): e1000547.

    Inhibition of bFGF-receptor type 2 increases kidney damage and suppresses nephrogenic protein expression after ischemic acute renal failure.
    Vio CP
    American journal of physiology. Regulatory, integrative and comparative physiology 294.3 (2008 Mar): R819-28.

    Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts.
    Schachner M
    The Journal of cell biology 159.4 (2002 Nov 25): 649-61.

    Evidence that Sry is expressed in pre-Sertoli cells and Sertoli and granulosa cells have a common precursor.
    Eicher EM
    Developmental biology 240.1 (2001 Dec 1): 92-107.

    p75NTR and Trk receptors are expressed in reciprocal patterns in a wide variety of non-neural tissues during rat embryonic development, indicating independent receptor functions.
    Vazquez L
    The Journal of comparative neurology 391.4 (1998 Feb 22): 407-28.

    An immunochemical, ultrastructural, and developmental characterization of the horizontal basal cells of rat olfactory epithelium.
    Schwob JE
    The Journal of comparative neurology 363.1 (1995 Dec 4): 129-46.

    Interactions of the chondroitin sulfate proteoglycan phosphacan, the extracellular domain of a receptor-type protein tyrosine phosphatase, with neurons, glia, and neural cell adhesion molecules.
    Margolis RU
    The Journal of cell biology 127.6 Pt 1 (1994 Dec): 1703-15.

    Dynamic expression patterns of tenascin, proteoglycans, and cell adhesion molecules during human hair follicle morphogenesis.
    Holbrook KA
    Developmental dynamics : an official publication of the American Association of Anatomists 199.2 (1994 Feb): 141-55.

    Spatiotemporal patterns of expression of NGF and the low-affinity NGF receptor in rat embryos suggest functional roles in tissue morphogenesis and myogenesis.
    Bothwell M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 12.3 (1992 Mar): 930-45.

    Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons.
    Jessell TM
    Neuron 1.2 (1988 Apr): 105-16.

    Afadin orients cell division to position the tubule lumen in developing renal tubules.
    Marciano DK
    Development (Cambridge, England) 144.19 (2017 Oct 1): 3511-3520.

    Functional Human Podocytes Generated in Organoids from Amniotic Fluid Stem Cells.
    Remuzzi G
    Journal of the American Society of Nephrology : JASN 27.5 (2016 May): 1400-11.

    Renal primordia activate kidney regenerative events in a rat model of progressive renal disease.
    Morigi M
    PloS one 10.3 (2015): e0120235.

    The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
    Schachner M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.42 (2013 Oct 16): 16828-45.

    Inhibiting angiotensin-converting enzyme promotes renal repair by limiting progenitor cell proliferation and restoring the glomerular architecture.
    Remuzzi G
    The American journal of pathology 179.2 (2011 Aug): 628-38.

    A yeast display immunoprecipitation method for efficient isolation and characterization of antigens.
    Shusta EV
    Journal of immunological methods 341.1-2 (2009 Feb 28): 117-26.

    Patterned neuropathologic events occurring in hyh congenital hydrocephalic mutant mice.
    Pérez-Fígares JM
    Journal of neuropathology and experimental neurology 66.12 (2007 Dec): 1082-92.

    Math1 controls cerebellar granule cell differentiation by regulating multiple components of the Notch signaling pathway.
    Ben-Arie N
    Development (Cambridge, England) 131.4 (2004 Feb): 903-13.

    Neural cell adhesion molecule (NCAM) association with PKCbeta2 via betaI spectrin is implicated in NCAM-mediated neurite outgrowth.
    Schachner M
    The Journal of cell biology 161.3 (2003 May 12): 625-39.

    NCAM-induced neurite outgrowth depends on binding of calmodulin to NCAM and on nuclear import of NCAM and fak fragments.
    Schachner M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.32 (2010 Aug 11): 10784-98.

    Dynamic change of neural cell adhesion molecule polysialylation on human neuroblastoma (IMR-32) and rat pheochromocytoma (PC-12) cells during growth and differentiation.
    Inoue Y
    The Journal of biological chemistry 277.31 (2002 Aug 2): 28200-11.

    HMGB4 is expressed by neuronal cells and affects the expression of genes involved in neural differentiation.
    Rauvala H
    Scientific reports 6. (2016 Sep 9): 32960.

    Basic fibroblast growth factor reduces functional and structural damage in chronic kidney disease.
    Vio CP
    American journal of physiology. Renal physiology 306.4 (2014 Feb 15): F430-41.

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