5F10

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$40.00
SKU: 5F10
View product citations for antibody 5F10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: allatostatin (Ast7)
Hybridoma Cells Available: Yes
Antigen Species: Cockroach
Depositor: Stay, B.
Isotype: MIgG1
Depositors Institution: University of Iowa
Host Species: mouse
Depositors Notes: Works on many insect species and many invertebrates. Some cross reaction with leu-callatostatin 1. Stains neural tissue, gut endocrine cells and ovary.
Positive Tested Species Reactivity: Cockroach, Copepod, Crab, Drosophila melanogaster, Earthworm, Insect, Invertebrate, Schmidtea mediterranea, Snail
Clonality: Monoclonal
Antigen Molecular Weight: Predicted: 39 kDa (propeptide), 1.3 kDa (ATS7)
Epitope Mapped: Yes
Immunogen: synthetic AST7 (APSGAQRLYGFGL), N-terminal coupled to BSA
Epitope Location or Sequence: likely YGFGL
Myeloma Strain: NS-1
Immunogen Sequence: peptide
Uniprot ID: P12764 
Additional Information: Stains type A allatostatins.
Antibody Registry ID: AB_528076 
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5F10 was deposited to the DSHB by Stay, B. (DSHB Hybridoma Product 5F10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Immunocytochemical localization of Diploptera punctata allatostatin-like peptide in Drosophila melanogaster.
    Stay B
    The Journal of comparative neurology 363.3 (1995 Dec 18): 475-88.

    Organization of the nervous system in the model planarian Schmidtea mediterranea: an immunocytochemical study.
    Cebrià F
    Neuroscience research 61.4 (2008 Aug): 375-84.

    Allatostatin in hemocytes of the cockroach Diploptera punctata.
    Stay B
    Cell and tissue research 290.1 (1997 Oct): 119-28.

    Generation of enteroendocrine cell diversity in midgut stem cell lineages.
    Micchelli CA
    Development (Cambridge, England) 142.4 (2015 Feb 15): 654-64.

    Evidence for a Phe-Gly-Leu-amide-like allatostatin in the beetle Tenebrio molitor.
    Stay B
    Peptides 31.3 (2010 Mar): 402-7.

    Context-dependent memory traces in the crab's mushroom bodies: Functional support for a common origin of high-order memory centers.
    Delorenzi A
    Proceedings of the National Academy of Sciences of the United States of America 113.49 (2016 Dec 6): E7957-E7965.

    Regeneration of neuronal cell types in Schmidtea mediterranea: an immunohistochemical and expression study.
    Cebrià F
    The International journal of developmental biology 56.1-3 (2012): 143-53.

    Morph-specific diurnal variation in allatostatin immunostaining in the corpora allata of Gryllus firmus: implications for the regulation of a morph-specific circadian rhythm for JH biosynthetic rate.
    Zera AJ
    Journal of insect physiology 56.3 (2010 Mar): 266-70.

    Distribution of C-type allatostatin (C-AST)-like immunoreactivity in the central nervous system of the copepod Calanus finmarchicus.
    Christie AE
    General and comparative endocrinology 167.2 (2010 Jun 1): 252-60.

    Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells.
    Xi R
    Nature cell biology 20.2 (2018 Feb): 152-161.

    IHC References

    Allatostatin-immunoreactive neurons projecting to the corpora allata of adult Diploptera punctata.
    Woodhead AP
    Cell and tissue research 270.1 (1992 Oct): 15-23.

    Immunocytochemical localization of Diploptera punctata allatostatin-like peptide in Drosophila melanogaster.
    Stay B
    The Journal of comparative neurology 363.3 (1995 Dec 18): 475-88.

    Allatostatin in hemocytes of the cockroach Diploptera punctata.
    Stay B
    Cell and tissue research 290.1 (1997 Oct): 119-28.

    Responsiveness of the adult cricket (Gryllus bimaculatus and Acheta domesticus) retrocerebral complex to allatostatin-1 from a cockroach, Diploptera punctata.
    Hoffmann KH
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 164.1 (1994): 23-31.

    ELISA References
    Epitope Map References
    All References

    Allatostatin-immunoreactive neurons projecting to the corpora allata of adult Diploptera punctata.
    Woodhead AP
    Cell and tissue research 270.1 (1992 Oct): 15-23.

    Immunocytochemical localization of Diploptera punctata allatostatin-like peptide in Drosophila melanogaster.
    Stay B
    The Journal of comparative neurology 363.3 (1995 Dec 18): 475-88.

    Allatostatin in hemocytes of the cockroach Diploptera punctata.
    Stay B
    Cell and tissue research 290.1 (1997 Oct): 119-28.

    Responsiveness of the adult cricket (Gryllus bimaculatus and Acheta domesticus) retrocerebral complex to allatostatin-1 from a cockroach, Diploptera punctata.
    Hoffmann KH
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 164.1 (1994): 23-31.

    Organization of the nervous system in the model planarian Schmidtea mediterranea: an immunocytochemical study.
    Cebrià F
    Neuroscience research 61.4 (2008 Aug): 375-84.

    Generation of enteroendocrine cell diversity in midgut stem cell lineages.
    Micchelli CA
    Development (Cambridge, England) 142.4 (2015 Feb 15): 654-64.

    Evidence for a Phe-Gly-Leu-amide-like allatostatin in the beetle Tenebrio molitor.
    Stay B
    Peptides 31.3 (2010 Mar): 402-7.

    Context-dependent memory traces in the crab's mushroom bodies: Functional support for a common origin of high-order memory centers.
    Delorenzi A
    Proceedings of the National Academy of Sciences of the United States of America 113.49 (2016 Dec 6): E7957-E7965.

    Regeneration of neuronal cell types in Schmidtea mediterranea: an immunohistochemical and expression study.
    Cebrià F
    The International journal of developmental biology 56.1-3 (2012): 143-53.

    Morph-specific diurnal variation in allatostatin immunostaining in the corpora allata of Gryllus firmus: implications for the regulation of a morph-specific circadian rhythm for JH biosynthetic rate.
    Zera AJ
    Journal of insect physiology 56.3 (2010 Mar): 266-70.

    Distribution of C-type allatostatin (C-AST)-like immunoreactivity in the central nervous system of the copepod Calanus finmarchicus.
    Christie AE
    General and comparative endocrinology 167.2 (2010 Jun 1): 252-60.

    Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells.
    Xi R
    Nature cell biology 20.2 (2018 Feb): 152-161.

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