AA12.1

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$40.00
SKU: AA12.1
View product citations for antibody AA12.1 on CiteAb

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Available: 140

DSHB Data Sheet

Catalog Fields

Antigen: tubulin (beta-)
Hybridoma Cells Available: Yes
Antigen Species: Naegleria gruberi
Depositor: Walsh, C.
Isotype: MIgM, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Pittsburgh
Positive Tested Species Reactivity: Broad species, Drosophila melanogaster, Human, Mammal, Mouse, Protist, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 50 kDa; Apparent: 55 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: TBB
Immunogen: flagellar axoneme
Alternate Gene Names:
Alternate Antibody Name: AA-12.1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3-X63Ag8
Epitope Location or Sequence:
Uniprot ID: P34108 
Immunogen Sequence: Total protein
Entrez Gene ID: 8863860 
Additional Characterization:
Antibody Registry ID: AB_579794 
Additional Information: This antibody may recognize more than one beta-tubulin isoform. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AA12.1 was deposited to the DSHB by Walsh, C. (DSHB Hybridoma Product AA12.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Organization of the ectodermal nervous structures in medusae: cubomedusae.
    Satterlie RA
    The Biological bulletin 226.1 (2014 Feb): 41-55.

    WB References

    mRNAs for alpha- and beta-tubulin and flagellar calmodulin are among those coordinately regulated when Naegleria gruberi amebae differentiate into flagellates.
    Walsh CJ
    The Journal of cell biology 105.3 (1987 Sep): 1303-9.

    A proteome map of axoglial specializations isolated and purified from human central nervous system.
    Colman DR
    Glia 58.16 (2010 Dec): 1949-60.

    Microtubules underlie dysfunction in duchenne muscular dystrophy.
    Ward CW
    Science signaling 5.236 (2012 Aug 7): ra56.

    Temperature-shock induction of multiple flagella induces additional synthesis of flagellum specific mRNAs and tubulin.
    Walsh CJ
    Experimental cell research 314.4 (2008 Feb 15): 896-902.

    Genetic silencing of Nrf2 enhances X-ROS in dysferlin-deficient muscle.
    Ward CW
    Frontiers in physiology 5. (2014): 57.

    Common genetic variants in the CLDN2 and PRSS1-PRSS2 loci alter risk for alcohol-related and sporadic pancreatitis.
    Devlin B
    Nature genetics 44.12 (2012 Dec): 1349-54.

    DPP-mediated TGFbeta signaling regulates juvenile hormone biosynthesis by activating the expression of juvenile hormone acid methyltransferase.
    Wang J
    Development (Cambridge, England) 138.11 (2011 Jun): 2283-91.

    Wnt signaling cross-talks with JH signaling by suppressing Met and gce expression.
    Wang J
    PloS one 6.11 (2011): e26772.

    NK Cell Maturation and Cytotoxicity Are Controlled by the Intramembrane Aspartyl Protease SPPL3.
    Pomerantz JL
    Journal of immunology (Baltimore, Md. : 1950) 196.6 (2016 Mar 15): 2614-26.

    A protease-independent function for SPPL3 in NFAT activation.
    Pomerantz JL
    Molecular and cellular biology 35.2 (2015 Jan): 451-67.

    ELISA References

    Synthesis and assembly of the cytoskeleton of Naegleria gruberi flagellates.
    Walsh C
    The Journal of cell biology 98.2 (1984 Feb): 449-56.

    All References

    Organization of the ectodermal nervous structures in medusae: cubomedusae.
    Satterlie RA
    The Biological bulletin 226.1 (2014 Feb): 41-55.

    mRNAs for alpha- and beta-tubulin and flagellar calmodulin are among those coordinately regulated when Naegleria gruberi amebae differentiate into flagellates.
    Walsh CJ
    The Journal of cell biology 105.3 (1987 Sep): 1303-9.

    A proteome map of axoglial specializations isolated and purified from human central nervous system.
    Colman DR
    Glia 58.16 (2010 Dec): 1949-60.

    Microtubules underlie dysfunction in duchenne muscular dystrophy.
    Ward CW
    Science signaling 5.236 (2012 Aug 7): ra56.

    Temperature-shock induction of multiple flagella induces additional synthesis of flagellum specific mRNAs and tubulin.
    Walsh CJ
    Experimental cell research 314.4 (2008 Feb 15): 896-902.

    Genetic silencing of Nrf2 enhances X-ROS in dysferlin-deficient muscle.
    Ward CW
    Frontiers in physiology 5. (2014): 57.

    Common genetic variants in the CLDN2 and PRSS1-PRSS2 loci alter risk for alcohol-related and sporadic pancreatitis.
    Devlin B
    Nature genetics 44.12 (2012 Dec): 1349-54.

    DPP-mediated TGFbeta signaling regulates juvenile hormone biosynthesis by activating the expression of juvenile hormone acid methyltransferase.
    Wang J
    Development (Cambridge, England) 138.11 (2011 Jun): 2283-91.

    Wnt signaling cross-talks with JH signaling by suppressing Met and gce expression.
    Wang J
    PloS one 6.11 (2011): e26772.

    NK Cell Maturation and Cytotoxicity Are Controlled by the Intramembrane Aspartyl Protease SPPL3.
    Pomerantz JL
    Journal of immunology (Baltimore, Md. : 1950) 196.6 (2016 Mar 15): 2614-26.

    A protease-independent function for SPPL3 in NFAT activation.
    Pomerantz JL
    Molecular and cellular biology 35.2 (2015 Jan): 451-67.

    Synthesis and assembly of the cytoskeleton of Naegleria gruberi flagellates.
    Walsh C
    The Journal of cell biology 98.2 (1984 Feb): 449-56.

    Drosophila clueless is highly expressed in larval neuroblasts, affects mitochondrial localization and suppresses mitochondrial oxidative damage.
    Cox RT
    PloS one 8.1 (2013): e54283.

    Mechanism and regulation of Cdc25/Twine protein destruction in embryonic cell-cycle remodeling.
    O'Farrell PH
    Current biology : CB 23.2 (2013 Jan 21): 118-26.

    Rapid centriole assembly in Naegleria reveals conserved roles for both de novo and mentored assembly.
    Fulton C
    Cytoskeleton (Hoboken, N.J.) 73.3 (2016 Mar): 109-16.

    Drosophila Xpd regulates Cdk7 localization, mitotic kinase activity, spindle dynamics, and chromosome segregation.
    Suter B
    PLoS genetics 6.3 (2010 Mar 12): e1000876.

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