AA12.1
Catalog Fields
Product Name/ID: AA12.1
Available to For-Profits: Yes
Alternate Antibody Name: AA-12.1
Gene Name: TBB
Ab Isotype: MIgM, kappa light chain
Gene Symbol:
Entrez Gene ID: 8863860
Clonality: Monoclonal
Immunogen: flagellar axoneme
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: P3-X63Ag8
Epitope Mapped: No
Antigen Name: tubulin (beta-)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/20/2005
Antigen Molecular Weight: Predicted: 50 kDa; Apparent: 55 kDa
Depositor: Walsh, C.
Antigen Sequence:
Depositor Institution: University of Pittsburgh
Antigen Species: Naegleria gruberi
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Broad species, C. elegans, Drosophila melanogaster, Human, Mammal, Mouse, Protist, Rat
Additional Information: This antibody may recognize more than one beta-tubulin isoform. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AA12.1 was deposited to the DSHB by Walsh, C. (DSHB Hybridoma Product AA12.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Synthesis and assembly of the cytoskeleton of Naegleria gruberi flagellates.
Walsh C
The Journal of cell biology 98.2 (1984 Feb): 449-56.
mRNAs for alpha- and beta-tubulin and flagellar calmodulin are among those coordinately regulated when Naegleria gruberi amebae differentiate into flagellates.
Walsh CJ
The Journal of cell biology 105.3 (1987 Sep): 1303-9.