(0) No Reviews yet
SKU: Abrupt
View product citations for antibody Abrupt on CiteAb

In Stock

Available: 27

DSHB Data Sheet

Catalog Fields

Antigen: Abrupt
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Crews, S.
Isotype: MIgG1
Depositors Institution: University of North Carolina - Chapel Hill
Host Species: mouse
Depositors Notes: Abrupt protein is also known as Protein Clueless.
Positive Tested Species Reactivity: Drosophila
Gene: ab
Antigen Molecular Weight: 95 kDa (Predicted)
Alternate Gene Names: clu
Immunogen: Recombinant 6XHis -Abrupt fusion protein
Clonality: Monoclonal
Myeloma Strain: P3x63 Ag8 653
Epitope Mapped: No
Uniprot ID: Q24174 
Immunogen Sequence: a.a. 278-498
Entrez Gene ID: 34560 
Additional Information: FBgn0264442. RRID: AB_528344
Antibody Registry ID: AB_528062 
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Abrupt was deposited to the DSHB by Crews, S. (DSHB Hybridoma Product Abrupt)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IHC References
  • All References
  • Ratings & Reviews

    No reviews available

    Be the first to Write a Review