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SKU: Abrupt
View product citations for antibody Abrupt on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Abrupt
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Crews, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of North Carolina - Chapel Hill
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Abrupt protein is also known as Protein Clueless.
Antigen Molecular Weight: 95 kDa (Predicted)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ab
Immunogen: Recombinant 6XHis -Abrupt fusion protein
Alternate Gene Names: clu
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3x63 Ag8 653
Epitope Location or Sequence:
Uniprot ID: Q24174 
Immunogen Sequence: a.a. 278-498
Entrez Gene ID: 34560 
Additional Characterization:  
Antibody Registry ID: AB_528062 
Additional Information: FBgn0264442. RRID: AB_528344
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Abrupt was deposited to the DSHB by Crews, S. (DSHB Hybridoma Product Abrupt)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

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