Anti-Argos 85/2/16

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SKU: Anti-Argos 85/2/16
View product citations for antibody Anti-Argos 85/2/16 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: argos
Hybridoma Cells Available: Yes
Antigen Species: drosophila
Depositor: Freeman, M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UNITED KINGDOM
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody doesn't appear to work for immunocytology (staining not convincing).
Antigen Molecular Weight: Predicted: 47 kDa; Apparent: 40 and 55 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: aos
Immunogen: Recombinat argos (lacking seven N-terminal aa)
Alternate Gene Names: argos, gil, arg, rlt, sty, CG4531
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS/O
Epitope Location or Sequence:
Uniprot ID: Q00805 
Immunogen Sequence: Full length protein
Entrez Gene ID: 39833 
Additional Characterization:  
Antibody Registry ID: AB_528088 
Additional Information: Argos is also known as protein giant-lens. This antibody was initially reported not to work well for immunocytology by the Depositor. However, it has been used with success for this technique in a number of publications.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Anti-Argos 85/2/16 was deposited to the DSHB by Freeman, M. (DSHB Hybridoma Product Anti-Argos 85/2/16)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Misexpression of the Drosophila argos gene, a secreted regulator of cell determination.
    Freeman M
    Development (Cambridge, England) 120.8 (1994 Aug): 2297-304.

    IF References
    WB References

    Misexpression of the Drosophila argos gene, a secreted regulator of cell determination.
    Freeman M
    Development (Cambridge, England) 120.8 (1994 Aug): 2297-304.

    klumpfuss regulates cell death in the Drosophila retina.
    Cagan R
    Mechanisms of development 121.6 (2004 Jun): 537-46.

    All References

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