Anti-Argos 85/2/16

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SKU: Anti-Argos 85/2/16
View product citations for antibody Anti-Argos 85/2/16 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: argos
Available to For-Profits: Yes
Antigen Species: drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Freeman, M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UNITED KINGDOM
Antigen Molecular Weight: Predicted: 47 kDa; Apparent: 40 and 55 kDa
Depositors Notes: This antibody doesn't appear to work for immunocytology (staining not convincing).
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinat argos (lacking seven N-terminal aa)
Gene: aos
Alternate Antibody Name:
Alternate Gene Names: argos, gil, arg, rlt, sty, CG4531
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS/O
Epitope Mapped: No
Uniprot ID: Q00805 
Epitope Location or Sequence:
Entrez Gene ID: 39833 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528088 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: Argos is also known as protein giant-lens. This antibody was initially reported not to work well for immunocytology by the Depositor. However, it has been used with success for this technique in a number of publications.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Anti-Argos 85/2/16 was deposited to the DSHB by Freeman, M. (DSHB Hybridoma Product Anti-Argos 85/2/16)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Misexpression of the Drosophila argos gene, a secreted regulator of cell determination.
    Freeman M
    Development (Cambridge, England) 120.8 (1994 Aug): 2297-304.

    IF References
    WB References

    Misexpression of the Drosophila argos gene, a secreted regulator of cell determination.
    Freeman M
    Development (Cambridge, England) 120.8 (1994 Aug): 2297-304.

    klumpfuss regulates cell death in the Drosophila retina.
    Cagan R
    Mechanisms of development 121.6 (2004 Jun): 537-46.

    All References

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