anti-Spitz

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$40.00
SKU: anti-Spitz
View product citations for antibody anti-Spitz on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Spitz, extracellular domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Shilo, B.-Z.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Weizmann Institute of Science
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 26 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: spi
Immunogen: Recombinant Drosophila spitz (N-terminal to dibasic signal located between the EGF and transmembrane domains)/GST fusion protein.
Alternate Gene Names: CG10334, spitz
Alternate Antibody Name: mAb Rtsspi, anti-spi
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: N-terminal region, to dibasic signal located between the EGF and transmembrane domains.
Uniprot ID: Q01083 
Immunogen Sequence: Partial protein
Entrez Gene ID: 35253 
Additional Characterization:
Antibody Registry ID: AB_528474 
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Spitz was deposited to the DSHB by Shilo, B.-Z. (DSHB Hybridoma Product anti-Spitz)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Genes & development
    Shilo BZ
    Genes & development 9.12 (1995 Jun 15): 1518-29.

    IF References

    Developmental biology
    Mollereau B
    Developmental biology 402.2 (2015 Jun 15): 208-15.

    Genes & development
    Shilo BZ
    Genes & development 16.2 (2002 Jan 15): 222-34.

    Developmental cell
    Treisman JE
    Developmental cell 10.2 (2006 Feb): 167-76.

    Development (Cambridge, England)
    Moses K
    Development (Cambridge, England) 132.21 (2005 Nov): 4697-707.

    Scientific reports
    Franch-Marro X
    Scientific reports 5. (2015 Dec 3): 17806.

    WB References

    Genes & development
    Shilo BZ
    Genes & development 9.12 (1995 Jun 15): 1518-29.

    Genes & development
    Shilo BZ
    Genes & development 16.2 (2002 Jan 15): 222-34.

    Developmental cell
    Treisman JE
    Developmental cell 10.2 (2006 Feb): 167-76.

    Journal of cell science
    Treisman JE
    Journal of cell science 126.Pt 19 (2013 Oct 1): 4469-78.

    Epitope Map References

    Genes & development
    Shilo BZ
    Genes & development 9.12 (1995 Jun 15): 1518-29.

    All References

    Developmental biology
    Mollereau B
    Developmental biology 402.2 (2015 Jun 15): 208-15.

    Genes & development
    Shilo BZ
    Genes & development 16.2 (2002 Jan 15): 222-34.

    Developmental cell
    Treisman JE
    Developmental cell 10.2 (2006 Feb): 167-76.

    Development (Cambridge, England)
    Moses K
    Development (Cambridge, England) 132.21 (2005 Nov): 4697-707.

    Scientific reports
    Franch-Marro X
    Scientific reports 5. (2015 Dec 3): 17806.

    Genes & development
    Shilo BZ
    Genes & development 9.12 (1995 Jun 15): 1518-29.

    Journal of cell science
    Treisman JE
    Journal of cell science 126.Pt 19 (2013 Oct 1): 4469-78.

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