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$45.00 to $425.00
SKU: anti-Spitz
View product citations for antibody anti-Spitz on CiteAb

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DSHB Data Sheet

Catalog Fields

Product Name/ID: anti-Spitz
Available to For-Profits: Yes
Alternate Antibody Name: mAb Rtsspi, anti-spi
Gene Name: spi
Ab Isotype: RIgG2a
Gene Symbol:
Antibody Registry ID: AB_528474 
Uniprot ID: Q01083 
Entrez Gene ID: 35253 
Clonality: Monoclonal
Immunogen: Recombinant Drosophila spitz (N-terminal to dibasic signal located between the EGF and transmembrane domains)/GST fusion protein.
Immunogen Sequence: Partial protein
Myeloma Strain: NSO
Epitope Mapped: Yes
Antigen Name: Spitz, extracellular domain
Epitope Location or Sequence: N-terminal region, to dibasic signal located between the EGF and transmembrane domains.
Alternate Antigen Name:
Deposit Date: 7/13/2005
Antigen Molecular Weight: Predicted: 26 kDa
Depositor: Shilo, B.-Z.
Antigen Sequence:
Depositor Institution: Weizmann Institute of Science
Antigen Species: Drosophila
Depositor Notes:
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Spitz was deposited to the DSHB by Shilo, B.-Z. (DSHB Hybridoma Product anti-Spitz)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    Epitope Map References
    All References

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