anti-Spitz
Catalog Fields
Antigen: Spitz, extracellular domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Shilo, B.-Z.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Weizmann Institute of Science
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Predicted Species Reactivity:
Gene: spi
Immunogen: Recombinant Drosophila spitz (N-terminal to dibasic signal located between the EGF and transmembrane domains)/GST fusion protein.
Alternate Gene Names: CG10334, spitz
Alternate Antibody Name: mAb Rtsspi, anti-spi
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: N-terminal region, to dibasic signal located between the EGF and transmembrane domains.
Uniprot ID: Q01083
Immunogen Sequence: Partial protein
Entrez Gene ID: 35253
Antibody Registry ID: AB_528474
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Spitz was deposited to the DSHB by Shilo, B.-Z. (DSHB Hybridoma Product anti-Spitz)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
7 References
Initial Publication
Secreted Spitz triggers the DER signaling pathway and is a limiting component in embryonic ventral ectoderm determination.
Shilo BZ
Genes & development 9.12 (1995 Jun 15): 1518-29.