anti-Spitz
Catalog Fields
Antigen: Spitz, extracellular domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Shilo, B.-Z.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Weizmann Institute of Science
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 26 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: spi
Immunogen: Recombinant Drosophila spitz (N-terminal to dibasic signal located between the EGF and transmembrane domains)/GST fusion protein.
Alternate Gene Names: CG10334, spitz
Alternate Antibody Name: mAb Rtsspi, anti-spi
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: N-terminal region, to dibasic signal located between the EGF and transmembrane domains.
Uniprot ID: Q01083
Immunogen Sequence: Partial protein
Entrez Gene ID: 35253
Additional Characterization:
Antibody Registry ID: AB_528474
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Spitz was deposited to the DSHB by Shilo, B.-Z. (DSHB Hybridoma Product anti-Spitz)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
7 References
Initial Publication
Secreted Spitz triggers the DER signaling pathway and is a limiting component in embryonic ventral ectoderm determination.
Shilo BZ
Genes and development 9.12 (1995 Jun 15): 1518-29.