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BP 106 anti-Neurotactin

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$40.00
SKU: BP 106 anti-Neurotactin
View product citations for antibody BP 106 anti-Neurotactin on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neurotactin (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis. The antibody stains restricted regions during the blastoderm stage and intensely stains cells at the ventral furrow. Expression expands to include almost all cells by the end of gastrulation, but later becomes restricted to, and very strongly expressed in, the developing nervous system. It is especially useful for looking at the pattern of neuroblasts. The antibody cannot be used to stain dissections without detergent. The antibody works well on Westerns, but not well for immunoprecipitations or affinity purifications. The corresponding gene has been isolated by expression cloning.
Antigen Molecular Weight: Predicted: 93 kDa; Apparent; 135 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Nrt
Immunogen: Membrane proteins from cultured embryonic Drosophila neuronal cells.
Alternate Gene Names: CG9704; dab; neu; neur
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Amino-terminal cytoplasmic domain; aa 1-280
Uniprot ID: P23654 
Immunogen Sequence: Total protein
Entrez Gene ID: 39873 
Additional Characterization:  
Antibody Registry ID: AB_528404 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BP 106 anti-Neurotactin was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product BP 106 anti-Neurotactin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

20 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Developmental changes in expression, subcellular distribution, and function of Drosophila N-cadherin, guided by a cell-intrinsic program during neuronal differentiation.
    Suzuki E
    Developmental biology 366.2 (2012 Jun 15): 204-17.

    Multipotent neural stem cells generate glial cells of the central complex through transit amplifying intermediate progenitors in Drosophila brain development.
    Reichert H
    Developmental biology 356.2 (2011 Aug 15): 553-65.

    Concomitant requirement for Notch and Jak/Stat signaling during neuro-epithelial differentiation in the Drosophila optic lobe.
    Hartenstein V
    Developmental biology 346.2 (2010 Oct 15): 284-95.

    Neural lineages of the Drosophila brain: a three-dimensional digital atlas of the pattern of lineage location and projection at the late larval stage.
    Hartenstein V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.20 (2006 May 17): 5534-53.

    Neuroblast lineage identification and lineage-specific Hox gene action during postembryonic development of the subesophageal ganglion in the Drosophila central brain.
    Reichert H
    Developmental biology 390.2 (2014 Jun 15): 102-15.

    Roles of Drosophila Kruppel-homolog 1 in neuronal morphogenesis.
    Lee T
    Developmental neurobiology 67.12 (2007 Oct): 1614-26.

    Bazooka mediates secondary axon morphology in Drosophila brain lineages.
    Hartenstein V
    Neural development 6. (2011 Apr 27): 16.

    Tracheal development in the Drosophila brain is constrained by glial cells.
    Hartenstein V
    Developmental biology 302.1 (2007 Feb 1): 169-80.

    Differential positioning of adherens junctions is associated with initiation of epithelial folding.
    Wieschaus EF
    Nature 484.7394 (2012 Mar 28): 390-3.

    Direct association of Bazooka/PAR-3 with the lipid phosphatase PTEN reveals a link between the PAR/aPKC complex and phosphoinositide signaling.
    Wodarz A
    Development (Cambridge, England) 132.7 (2005 Apr): 1675-86.

    The proximal half of the Drosophila E-cadherin extracellular region is dispensable for many cadherin-dependent events but required for ventral furrow formation.
    Oda H
    Genes to cells : devoted to molecular & cellular mechanisms 15.3 (2010 Mar): 193-208.

    Developmental control of nuclear morphogenesis and anchoring by charleston, identified in a functional genomic screen of Drosophila cellularisation.
    Lecuit T
    Development (Cambridge, England) 133.4 (2006 Feb): 711-23.

    Drak Is Required for Actomyosin Organization During Drosophila Cellularization.
    Thomas JH
    G3 (Bethesda, Md.) 6.4 (2016 Apr 7): 819-28.

    Cell shape change and invagination of the cephalic furrow involves reorganization of F-actin.
    Thomas JH
    Developmental biology 402.2 (2015 Jun 15): 192-207.

    Structure and development of the subesophageal zone of the Drosophila brain. II. Sensory compartments.
    Hartenstein V
    The Journal of comparative neurology 526.1 (2018 Jan 1): 33-58.

    WB References
    IHC References
    Epitope Map References
    All References

    Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis.
    Goodman CS
    Development (Cambridge, England) 110.4 (1990 Dec): 1327-40.

    A Drosophila receptor tyrosine phosphatase expressed in the embryonic CNS and larval optic lobes is a member of the set of proteins bearing the "HRP" carbohydrate epitope.
    Zinn K
    The Journal of neuroscience : the official journal of the Society for Neuroscience 14.12 (1994 Dec): 7272-83.

    Genetic induction and mechanochemical propagation of a morphogenetic wave.
    Lecuit T
    Nature 572.7770 (2019 Aug): 467-473.

    Developmental changes in expression, subcellular distribution, and function of Drosophila N-cadherin, guided by a cell-intrinsic program during neuronal differentiation.
    Suzuki E
    Developmental biology 366.2 (2012 Jun 15): 204-17.

    Multipotent neural stem cells generate glial cells of the central complex through transit amplifying intermediate progenitors in Drosophila brain development.
    Reichert H
    Developmental biology 356.2 (2011 Aug 15): 553-65.

    Concomitant requirement for Notch and Jak/Stat signaling during neuro-epithelial differentiation in the Drosophila optic lobe.
    Hartenstein V
    Developmental biology 346.2 (2010 Oct 15): 284-95.

    Neural lineages of the Drosophila brain: a three-dimensional digital atlas of the pattern of lineage location and projection at the late larval stage.
    Hartenstein V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.20 (2006 May 17): 5534-53.

    Neuroblast lineage identification and lineage-specific Hox gene action during postembryonic development of the subesophageal ganglion in the Drosophila central brain.
    Reichert H
    Developmental biology 390.2 (2014 Jun 15): 102-15.

    Roles of Drosophila Kruppel-homolog 1 in neuronal morphogenesis.
    Lee T
    Developmental neurobiology 67.12 (2007 Oct): 1614-26.

    Bazooka mediates secondary axon morphology in Drosophila brain lineages.
    Hartenstein V
    Neural development 6. (2011 Apr 27): 16.

    Tracheal development in the Drosophila brain is constrained by glial cells.
    Hartenstein V
    Developmental biology 302.1 (2007 Feb 1): 169-80.

    Differential positioning of adherens junctions is associated with initiation of epithelial folding.
    Wieschaus EF
    Nature 484.7394 (2012 Mar 28): 390-3.

    Direct association of Bazooka/PAR-3 with the lipid phosphatase PTEN reveals a link between the PAR/aPKC complex and phosphoinositide signaling.
    Wodarz A
    Development (Cambridge, England) 132.7 (2005 Apr): 1675-86.

    The proximal half of the Drosophila E-cadherin extracellular region is dispensable for many cadherin-dependent events but required for ventral furrow formation.
    Oda H
    Genes to cells : devoted to molecular & cellular mechanisms 15.3 (2010 Mar): 193-208.

    Developmental control of nuclear morphogenesis and anchoring by charleston, identified in a functional genomic screen of Drosophila cellularisation.
    Lecuit T
    Development (Cambridge, England) 133.4 (2006 Feb): 711-23.

    Drak Is Required for Actomyosin Organization During Drosophila Cellularization.
    Thomas JH
    G3 (Bethesda, Md.) 6.4 (2016 Apr 7): 819-28.

    Cell shape change and invagination of the cephalic furrow involves reorganization of F-actin.
    Thomas JH
    Developmental biology 402.2 (2015 Jun 15): 192-207.

    Structure and development of the subesophageal zone of the Drosophila brain. II. Sensory compartments.
    Hartenstein V
    The Journal of comparative neurology 526.1 (2018 Jan 1): 33-58.

    Interactions between the secreted protein Amalgam, its transmembrane receptor Neurotactin and the Abelson tyrosine kinase affect axon pathfinding.
    Seeger MA
    Development (Cambridge, England) 130.14 (2003 Jul): 3217-26.

    Interaction between EGFR signaling and DE-cadherin during nervous system morphogenesis.
    Hartenstein V
    Development (Cambridge, England) 129.17 (2002 Sep): 3983-94.

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