BP 106 anti-Neurotactin
Catalog Fields
Product Name/ID: BP 106 anti-Neurotactin
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Nrt
Ab Isotype: MIgG2a
Gene Symbol:
Entrez Gene ID: 39873
Clonality: Monoclonal
Immunogen: Membrane proteins from cultured embryonic Drosophila neuronal cells.
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: neurotactin (Drosophila)
Epitope Location or Sequence: Amino-terminal cytoplasmic domain; aa 1-280
Alternate Antigen Name:
Deposit Date: 2/1/1994
Antigen Molecular Weight: Predicted: 93 kDa; Apparent; 135 kDa
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis. The antibody stains restricted regions during the blastoderm stage and intensely stains cells at the ventral furrow. Expression expands to include almost all cells by the end of gastrulation, but later becomes restricted to, and very strongly expressed in, the developing nervous system. It is especially useful for looking at the pattern of neuroblasts. The antibody cannot be used to stain dissections without detergent. The antibody works well on Westerns, but not well for immunoprecipitations or affinity purifications. The corresponding gene has been isolated by expression cloning.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BP 106 anti-Neurotactin was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product BP 106 anti-Neurotactin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
20 References
Initial Publication
Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis.
Goodman CS
Development (Cambridge, England) 110.4 (1990 Dec): 1327-40.