BP 106 anti-Neurotactin
Catalog Fields
Antigen: neurotactin (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis. The antibody stains restricted regions during the blastoderm stage and intensely stains cells at the ventral furrow. Expression expands to include almost all cells by the end of gastrulation, but later becomes restricted to, and very strongly expressed in, the developing nervous system. It is especially useful for looking at the pattern of neuroblasts. The antibody cannot be used to stain dissections without detergent. The antibody works well on Westerns, but not well for immunoprecipitations or affinity purifications. The corresponding gene has been isolated by expression cloning.
Predicted Species Reactivity:
Gene: Nrt
Immunogen: Membrane proteins from cultured embryonic Drosophila neuronal cells.
Alternate Gene Names: CG9704; dab; neu; neur
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Amino-terminal cytoplasmic domain; aa 1-280
Uniprot ID: P23654
Immunogen Sequence: Total protein
Entrez Gene ID: 39873
Antibody Registry ID: AB_528404
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BP 106 anti-Neurotactin was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product BP 106 anti-Neurotactin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
20 References
Initial Publication
Drosophila neurotactin, a surface glycoprotein with homology to serine esterases, is dynamically expressed during embryogenesis.
Goodman CS
Development (Cambridge, England) 110.4 (1990 Dec): 1327-40.