Broad-Z1 (Z1.3C11.OA1)
Catalog Fields
Antigen: Broad (Z1)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Guild, G.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Pennsylvania
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Threre are 3 reported isoforms for Z1. Predicted: 70, 72, and 77 kDa; Apparent: 81 and 91 kDa
Predicted Species Reactivity:
Gene: br
Immunogen: Recombinant, His-tagged fusion protein derived from cDNA clone BRcore-Q-Z1 corresponding to isoform 2 of BR-C [Uniprot ID Q01295-2]
Alternate Gene Names: CG11509; BR-C; BR-C Z1; Br-C Z2; Br-C-Z3; npr1; PP1; PP2; rbp; rdp; rds; uq
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0-Ag14
Epitope Location or Sequence: aa 432-572 of isoform 2 [Uniprot ID Q01295-2] which is common to all three Z1 isoforms
Uniprot ID: Q01295
Immunogen Sequence: Total protein
Entrez Gene ID: 44505
Antibody Registry ID: AB_528105
Additional Information: Specific for the Z1 isoforms of the broad protein complex of transcription factors. There are three reported Z1 BR-C isoforms.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Broad-Z1 (Z1.3C11.OA1) was deposited to the DSHB by Guild, G. (DSHB Hybridoma Product Broad-Z1 (Z1.3C11.OA1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis.
Guild GM
Development (Cambridge, England) 120.11 (1994 Nov): 3275-87.