Broad-Z1 (Z1.3C11.OA1)

(0) No Reviews yet
$40.00
SKU: Broad-Z1 (Z1.3C11.OA1)
View product citations for antibody Broad-Z1 (Z1.3C11.OA1) on CiteAb

In Stock

Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Broad (Z1)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Guild, G.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Pennsylvania
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Threre are 3 reported isoforms for Z1. Predicted: 70, 72, and 77 kDa; Apparent: 81 and 91 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: br
Immunogen: Recombinant, His-tagged fusion protein derived from cDNA clone BRcore-Q-Z1 corresponding to isoform 2 of BR-C [Uniprot ID Q01295-2]
Alternate Gene Names: CG11509; BR-C; BR-C Z1; Br-C Z2; Br-C-Z3; npr1; PP1; PP2; rbp; rdp; rds; uq
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0-Ag14
Epitope Location or Sequence: aa 432-572 of isoform 2 [Uniprot ID Q01295-2] which is common to all three Z1 isoforms
Uniprot ID: Q01295 
Immunogen Sequence: Total protein
Entrez Gene ID: 44505 
Additional Characterization:  
Antibody Registry ID: AB_528105 
Additional Information: Specific for the Z1 isoforms of the broad protein complex of transcription factors. There are three reported Z1 BR-C isoforms.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Broad-Z1 (Z1.3C11.OA1) was deposited to the DSHB by Guild, G. (DSHB Hybridoma Product Broad-Z1 (Z1.3C11.OA1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Ecdysone pathway is required for furrow progression in the developing Drosophila eye.
    Moses K
    Development (Cambridge, England) 125.14 (1998 Jul): 2653-64.

    Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye.
    Moses K
    Development (Cambridge, England) 128.1 (2001 Jan): 1-11.

    Broad-complex transcription factors regulate thoracic muscle attachment in Drosophila.
    Restifo LL
    Developmental biology 181.2 (1997 Jan 15): 168-85.

    A comparative study of Desktop, Fishtank, and Cave systems for the exploration of volume rendered confocal data sets.
    Slater M
    IEEE transactions on visualization and computer graphics 14.3 (2008 May-Jun): 551-63.

    Translational control by the DEAD Box RNA helicase belle regulates ecdysone-triggered transcriptional cascades.
    Bashirullah A
    PLoS genetics 8.11 (2012): e1003085.

    The role of Dpp and its inhibitors during eggshell patterning in Drosophila.
    Roth S
    Development (Cambridge, England) 134.12 (2007 Jun): 2261-71.

    Genome wide analysis of transcript levels after perturbation of the EGFR pathway in the Drosophila ovary.
    Ruohola-Baker H
    Developmental dynamics : an official publication of the American Association of Anatomists 232.3 (2005 Mar): 709-24.

    The RXR ortholog USP suppresses early metamorphic processes in Drosophila in the absence of ecdysteroids.
    Truman JW
    Development (Cambridge, England) 127.6 (2000 Mar): 1151-9.

    Broad Complex isoforms have unique distributions during central nervous system metamorphosis in Drosophila melanogaster.
    Restifo LL
    The Journal of comparative neurology 517.1 (2009 Nov 1): 15-36.

    Coordinated regulation of niche and stem cell precursors by hormonal signaling.
    Gilboa L
    PLoS biology 9.11 (2011 Nov): e1001202.

    The COP9 signalosome converts temporal hormone signaling to spatial restriction on neural competence.
    Pi H
    PLoS genetics 10.11 (2014 Nov): e1004760.

    WB References
    IHC References

    Ecdysone pathway is required for furrow progression in the developing Drosophila eye.
    Moses K
    Development (Cambridge, England) 125.14 (1998 Jul): 2653-64.

    Epitope Map References
    All References

    Ecdysone pathway is required for furrow progression in the developing Drosophila eye.
    Moses K
    Development (Cambridge, England) 125.14 (1998 Jul): 2653-64.

    Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye.
    Moses K
    Development (Cambridge, England) 128.1 (2001 Jan): 1-11.

    Broad-complex transcription factors regulate thoracic muscle attachment in Drosophila.
    Restifo LL
    Developmental biology 181.2 (1997 Jan 15): 168-85.

    A comparative study of Desktop, Fishtank, and Cave systems for the exploration of volume rendered confocal data sets.
    Slater M
    IEEE transactions on visualization and computer graphics 14.3 (2008 May-Jun): 551-63.

    Translational control by the DEAD Box RNA helicase belle regulates ecdysone-triggered transcriptional cascades.
    Bashirullah A
    PLoS genetics 8.11 (2012): e1003085.

    The role of Dpp and its inhibitors during eggshell patterning in Drosophila.
    Roth S
    Development (Cambridge, England) 134.12 (2007 Jun): 2261-71.

    Genome wide analysis of transcript levels after perturbation of the EGFR pathway in the Drosophila ovary.
    Ruohola-Baker H
    Developmental dynamics : an official publication of the American Association of Anatomists 232.3 (2005 Mar): 709-24.

    The RXR ortholog USP suppresses early metamorphic processes in Drosophila in the absence of ecdysteroids.
    Truman JW
    Development (Cambridge, England) 127.6 (2000 Mar): 1151-9.

    Broad Complex isoforms have unique distributions during central nervous system metamorphosis in Drosophila melanogaster.
    Restifo LL
    The Journal of comparative neurology 517.1 (2009 Nov 1): 15-36.

    Coordinated regulation of niche and stem cell precursors by hormonal signaling.
    Gilboa L
    PLoS biology 9.11 (2011 Nov): e1001202.

    The COP9 signalosome converts temporal hormone signaling to spatial restriction on neural competence.
    Pi H
    PLoS genetics 10.11 (2014 Nov): e1004760.

    Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis.
    Guild GM
    Development (Cambridge, England) 120.11 (1994 Nov): 3275-87.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review