Broad-core (25E9.D7)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Broad-core (25E9.D7)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: br
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528104 
Uniprot ID: Q01295 
RRID:  
Entrez Gene ID: 44505 
Clonality: Monoclonal
Immunogen: Recombinant, His-tagged fusion protein derived from cDNA clone BRcore-Z2 corresponding to isoform 4 of BR-C [Uniprot ID Q01295-4]
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: Sp2/0-Ag14
Epitope Mapped: Yes
Antigen Name: Broad (core)
Epitope Location or Sequence: The CORE region common to all isoforms (aa 1-431)
Alternate Antigen Name:
Deposit Date: 10/15/2003
Antigen Molecular Weight: There are 6 reported isoforms. Predicted: 55, 70, 72, 74, 77, and 92 kDa; Apparent: 57, 64, 81, 91, and 118 kDa
Depositor: Guild, G.
Antigen Sequence:
Depositor Institution: University of Pennsylvania
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Binds to all 6 isoforms of the broad protein complex of transcription factors (BR-C).
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Broad-core (25E9.D7) was deposited to the DSHB by Guild, G. (DSHB Hybridoma Product Broad-core (25E9.D7))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

14 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis.
    Guild GM
    Development (Cambridge, England) 120.11 (1994 Nov): 3275-87.

    Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte.
    Nilson LA
    Development (Cambridge, England) 132.10 (2005 May): 2345-53.

    Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila.
    Segraves WA
    Development (Cambridge, England) 126.20 (1999 Oct): 4581-9.

    Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye.
    Moses K
    Development (Cambridge, England) 128.1 (2001 Jan): 1-11.

    Broad Complex isoforms have unique distributions during central nervous system metamorphosis in Drosophila melanogaster.
    Restifo LL
    The Journal of comparative neurology 517.1 (2009 Nov 1): 15-36.

    Broad relays hormone signals to regulate stem cell differentiation in Drosophila midgut during metamorphosis.
    Hou SX
    Development (Cambridge, England) 139.21 (2012 Nov): 3917-25.

    The ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs.
    Riddiford LM
    Development (Cambridge, England) 136.14 (2009 Jul): 2345-53.

    Coordinated regulation of niche and stem cell precursors by hormonal signaling.
    Gilboa L
    PLoS biology 9.11 (2011 Nov): e1001202.

    Wnt signaling cross-talks with JH signaling by suppressing Met and gce expression.
    Wang J
    PloS one 6.11 (2011): e26772.

    DPP-mediated TGFbeta signaling regulates juvenile hormone biosynthesis by activating the expression of juvenile hormone acid methyltransferase.
    Wang J
    Development (Cambridge, England) 138.11 (2011 Jun): 2283-91.

    Determination of EGFR Signaling Output by Opposing Gradients of BMP and JAK/STAT Activity.
    Nilson LA
    Current biology : CB 26.19 (2016 Oct 10): 2572-2582.

    The conserved microRNA miR-8-3p coordinates the expression of V-ATPase subunits to regulate ecdysone biosynthesis for Drosophila metamorphosis.
    Lee YS
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34.5 (2020 May): 6449-6465.

    The steroid-induced microRNA let-7 regulates developmental growth by targeting cdc7 in the Drosophila fat body.
    Liu SN
    Insect science 28.6 (2021 Dec): 1621-1632.

    Antagonistic role of the BTB-zinc finger transcription factors Chinmo and Broad-Complex in the juvenile/pupal transition and in growth control.
    Franch-Marro X
    eLife 12. (2023 Apr 28): .

    WB References
    Epitope Map References
    All References

    Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis.
    Guild GM
    Development (Cambridge, England) 120.11 (1994 Nov): 3275-87.

    Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte.
    Nilson LA
    Development (Cambridge, England) 132.10 (2005 May): 2345-53.

    Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila.
    Segraves WA
    Development (Cambridge, England) 126.20 (1999 Oct): 4581-9.

    Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye.
    Moses K
    Development (Cambridge, England) 128.1 (2001 Jan): 1-11.

    Broad Complex isoforms have unique distributions during central nervous system metamorphosis in Drosophila melanogaster.
    Restifo LL
    The Journal of comparative neurology 517.1 (2009 Nov 1): 15-36.

    Broad relays hormone signals to regulate stem cell differentiation in Drosophila midgut during metamorphosis.
    Hou SX
    Development (Cambridge, England) 139.21 (2012 Nov): 3917-25.

    The ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs.
    Riddiford LM
    Development (Cambridge, England) 136.14 (2009 Jul): 2345-53.

    Coordinated regulation of niche and stem cell precursors by hormonal signaling.
    Gilboa L
    PLoS biology 9.11 (2011 Nov): e1001202.

    Wnt signaling cross-talks with JH signaling by suppressing Met and gce expression.
    Wang J
    PloS one 6.11 (2011): e26772.

    DPP-mediated TGFbeta signaling regulates juvenile hormone biosynthesis by activating the expression of juvenile hormone acid methyltransferase.
    Wang J
    Development (Cambridge, England) 138.11 (2011 Jun): 2283-91.

    Determination of EGFR Signaling Output by Opposing Gradients of BMP and JAK/STAT Activity.
    Nilson LA
    Current biology : CB 26.19 (2016 Oct 10): 2572-2582.

    The conserved microRNA miR-8-3p coordinates the expression of V-ATPase subunits to regulate ecdysone biosynthesis for Drosophila metamorphosis.
    Lee YS
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34.5 (2020 May): 6449-6465.

    The steroid-induced microRNA let-7 regulates developmental growth by targeting cdc7 in the Drosophila fat body.
    Liu SN
    Insect science 28.6 (2021 Dec): 1621-1632.

    Antagonistic role of the BTB-zinc finger transcription factors Chinmo and Broad-Complex in the juvenile/pupal transition and in growth control.
    Franch-Marro X
    eLife 12. (2023 Apr 28): .

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