Broad-core (25E9.D7)
Catalog Fields
Clone ID/Product Name: Broad-core (25E9.D7)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: br
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 44505
Clonality: Monoclonal
Immunogen: Recombinant, His-tagged fusion protein derived from cDNA clone BRcore-Z2 corresponding to isoform 4 of BR-C [Uniprot ID Q01295-4]
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: Sp2/0-Ag14
Epitope Mapped: Yes
Antigen Name: Broad (core)
Epitope Location or Sequence: The CORE region common to all isoforms (aa 1-431)
Alternate Antigen Name:
Deposit Date: 10/15/2003
Antigen Molecular Weight: There are 6 reported isoforms. Predicted: 55, 70, 72, 74, 77, and 92 kDa; Apparent: 57, 64, 81, 91, and 118 kDa
Depositor: Guild, G.
Antigen Sequence:
Depositor Institution: University of Pennsylvania
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Binds to all 6 isoforms of the broad protein complex of transcription factors (BR-C).
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Broad-core (25E9.D7) was deposited to the DSHB by Guild, G. (DSHB Hybridoma Product Broad-core (25E9.D7))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
14 References
Initial Publication
Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis.
Guild GM
Development (Cambridge, England) 120.11 (1994 Nov): 3275-87.