Broad-core (25E9.D7)

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$40.00
SKU: Broad-core (25E9.D7)
View product citations for antibody Broad-core (25E9.D7) on CiteAb

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Available: 111

DSHB Data Sheet

Catalog Fields

Antigen: Broad (core)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Guild, G.
Isotype: MIgG1, kappa light chain
Depositors Institution: University of Pennsylvania
Host Species: mouse
Gene: br
Positive Tested Species Reactivity: Drosophila
Alternate Gene Names: CG11509; BR-C; BR-C Z1; Br-C Z2; Br-C-Z3; npr1; PP1; PP2; rbp; rdp; rds; uq
Antigen Molecular Weight: There are 6 reported isoforms. Predicted: 55, 70, 72, 74, 77, and 92 kDa; Apparent: 57, 64, 81, 91, and 118 kDa
Clonality: Monoclonal
Immunogen: Recombinant, His-tagged fusion protein derived from cDNA clone BRcore-Z2 corresponding to isoform 4 of BR-C [Uniprot ID Q01295-4]
Epitope Mapped: Yes
Myeloma Strain: Sp2/0-Ag14
Epitope Location or Sequence: The CORE region common to all isoforms (aa 1-431)
Uniprot ID: Q01295 
Immunogen Sequence: Total protein
Entrez Gene ID: 44505 
Additional Information: Binds to all 6 isoforms of the broad protein complex of transcription factors (BR-C).
Antibody Registry ID: AB_528104 
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Broad-core (25E9.D7) was deposited to the DSHB by Guild, G. (DSHB Hybridoma Product Broad-core (25E9.D7))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis.
    Guild GM
    Development (Cambridge, England) 120.11 (1994 Nov): 3275-87.

    Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte.
    Nilson LA
    Development (Cambridge, England) 132.10 (2005 May): 2345-53.

    Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila.
    Segraves WA
    Development (Cambridge, England) 126.20 (1999 Oct): 4581-9.

    Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye.
    Moses K
    Development (Cambridge, England) 128.1 (2001 Jan): 1-11.

    Broad Complex isoforms have unique distributions during central nervous system metamorphosis in Drosophila melanogaster.
    Restifo LL
    The Journal of comparative neurology 517.1 (2009 Nov 1): 15-36.

    Broad relays hormone signals to regulate stem cell differentiation in Drosophila midgut during metamorphosis.
    Hou SX
    Development (Cambridge, England) 139.21 (2012 Nov): 3917-25.

    The ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs.
    Riddiford LM
    Development (Cambridge, England) 136.14 (2009 Jul): 2345-53.

    Coordinated regulation of niche and stem cell precursors by hormonal signaling.
    Gilboa L
    PLoS biology 9.11 (2011 Nov): e1001202.

    Wnt signaling cross-talks with JH signaling by suppressing Met and gce expression.
    Wang J
    PloS one 6.11 (2011): e26772.

    DPP-mediated TGFbeta signaling regulates juvenile hormone biosynthesis by activating the expression of juvenile hormone acid methyltransferase.
    Wang J
    Development (Cambridge, England) 138.11 (2011 Jun): 2283-91.

    Determination of EGFR Signaling Output by Opposing Gradients of BMP and JAK/STAT Activity.
    Nilson LA
    Current biology : CB 26.19 (2016 Oct 10): 2572-2582.

    WB References
    Epitope Map References
    All References

    Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis.
    Guild GM
    Development (Cambridge, England) 120.11 (1994 Nov): 3275-87.

    Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte.
    Nilson LA
    Development (Cambridge, England) 132.10 (2005 May): 2345-53.

    Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila.
    Segraves WA
    Development (Cambridge, England) 126.20 (1999 Oct): 4581-9.

    Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye.
    Moses K
    Development (Cambridge, England) 128.1 (2001 Jan): 1-11.

    Broad Complex isoforms have unique distributions during central nervous system metamorphosis in Drosophila melanogaster.
    Restifo LL
    The Journal of comparative neurology 517.1 (2009 Nov 1): 15-36.

    Broad relays hormone signals to regulate stem cell differentiation in Drosophila midgut during metamorphosis.
    Hou SX
    Development (Cambridge, England) 139.21 (2012 Nov): 3917-25.

    The ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs.
    Riddiford LM
    Development (Cambridge, England) 136.14 (2009 Jul): 2345-53.

    Coordinated regulation of niche and stem cell precursors by hormonal signaling.
    Gilboa L
    PLoS biology 9.11 (2011 Nov): e1001202.

    Wnt signaling cross-talks with JH signaling by suppressing Met and gce expression.
    Wang J
    PloS one 6.11 (2011): e26772.

    DPP-mediated TGFbeta signaling regulates juvenile hormone biosynthesis by activating the expression of juvenile hormone acid methyltransferase.
    Wang J
    Development (Cambridge, England) 138.11 (2011 Jun): 2283-91.

    Determination of EGFR Signaling Output by Opposing Gradients of BMP and JAK/STAT Activity.
    Nilson LA
    Current biology : CB 26.19 (2016 Oct 10): 2572-2582.

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