Connectin C1.427

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SKU: Connectin C1.427
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DSHB Data Sheet

Catalog Fields

Product Name/ID: Connectin C1.427
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Con
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_10660830 
Uniprot ID: Q01819 
RRID:  
Entrez Gene ID: 38590 
Clonality: Monoclonal
Immunogen: Recombinant aa 43-632 of the mature Connectin protein (missing the N-terminus 42 aa, and 2 aa at the C-terminus)
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: NSO
Epitope Mapped: Yes
Antigen Name: Connectin
Epitope Location or Sequence: aa 43-632
Alternate Antigen Name:
Deposit Date: 5/25/2010
Antigen Molecular Weight: Predicted: 71 kDa
Depositor: White, R.
Antigen Sequence:
Depositor Institution: University of Cambridge
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Connectin C1.427 was deposited to the DSHB by White, R. (DSHB Hybridoma Product Connectin C1.427)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Differential and redundant functions of gooseberry and gooseberry neuro in the central nervous system and segmentation of the Drosophila embryo.
    Noll M
    Developmental biology 382.1 (2013 Oct 1): 209-23.

    Cholinergic circuits integrate neighboring visual signals in a Drosophila motion detection pathway.
    Meinertzhagen IA
    Current biology : CB 21.24 (2011 Dec 20): 2077-84.

    Head region of unconventional myosin I family members is responsible for the organ-specificity of their roles in left-right polarity in Drosophila.
    Matsuno K
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3528-37.

    Mapping chromatic pathways in the Drosophila visual system.
    Lee CH
    The Journal of comparative neurology 524.2 (2016 Feb 1): 213-27.

    WB References
    IHC References
    FB References
    Epitope Map References
    All References

    The cell adhesion molecule, connectin, and the development of the Drosophila neuromuscular system.
    White RA
    Journal of cell science 107 ( Pt 1). (1994 Jan): 321-8.

    Dosage-sensitive and complementary functions of roundabout and commissureless control axon crossing of the CNS midline.
    Tear G
    Neuron 20.1 (1998 Jan): 25-33.

    Connectin mediates adhesion in Drosophila.
    White RA
    Neuron 18.6 (1997 Jun): 873-80.

    Receptor tyrosine phosphatases regulate axon guidance across the midline of the Drosophila embryo.
    Zinn K
    Development (Cambridge, England) 127.4 (2000 Feb): 801-12.

    Muscle founder cells regulate defasciculation and targeting of motor axons in the Drosophila embryo.
    Bate M
    Current biology : CB 9.11 (1999 Jun 3): 589-92.

    split ends encodes large nuclear proteins that regulate neuronal cell fate and axon extension in the Drosophila embryo.
    Kolodziej P
    Development (Cambridge, England) 127.7 (2000 Apr): 1517-29.

    Axon repulsion from the midline of the Drosophila CNS requires slit function.
    Jacobs JR
    Development (Cambridge, England) 126.11 (1999 Jun): 2475-81.

    Differential and redundant functions of gooseberry and gooseberry neuro in the central nervous system and segmentation of the Drosophila embryo.
    Noll M
    Developmental biology 382.1 (2013 Oct 1): 209-23.

    Cholinergic circuits integrate neighboring visual signals in a Drosophila motion detection pathway.
    Meinertzhagen IA
    Current biology : CB 21.24 (2011 Dec 20): 2077-84.

    Head region of unconventional myosin I family members is responsible for the organ-specificity of their roles in left-right polarity in Drosophila.
    Matsuno K
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3528-37.

    Mapping chromatic pathways in the Drosophila visual system.
    Lee CH
    The Journal of comparative neurology 524.2 (2016 Feb 1): 213-27.

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