Connectin C1.427
Catalog Fields
Antigen: Connectin
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: White, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Predicted Species Reactivity:
Gene: Con
Immunogen: Recombinant aa 43-632 of the mature Connectin protein (missing the N-terminus 42 aa, and 2 aa at the C-terminus)
Alternate Gene Names: CG7503, conn
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: aa 43-632
Uniprot ID: Q01819
Immunogen Sequence: Partial protein
Entrez Gene ID: 38590
Antibody Registry ID: AB_10660830
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Connectin C1.427 was deposited to the DSHB by White, R. (DSHB Hybridoma Product Connectin C1.427)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
The cell adhesion molecule, connectin, and the development of the Drosophila neuromuscular system.
White RA
Journal of cell science 107 ( Pt 1). (1994 Jan): 321-8.