E4

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$40.00
SKU: E4
View product citations for antibody E4 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Reelin
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Goffinet, A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Louvain, Belgium
Positive Tested Species Reactivity: Mouse, Rodent
Depositors Notes: E4 was generated using the Edinburgh allele reelin mutant mouse. This antibody recognizes the same epitope as anti-reelin G10.
Antigen Molecular Weight: 387.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Reln
Immunogen: Protein H domain N-terminus
Alternate Gene Names: Rl
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: The E4 epitope lies between a.a. 164-245
Uniprot ID: Q60841 
Immunogen Sequence: Synthetic peptide-BSA conjugate
Entrez Gene ID: 19699 
Additional Characterization:  
Antibody Registry ID: AB_1157891 
Additional Information: RRID:AB_1157891
Recommended Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
E4 was deposited to the DSHB by Goffinet, A. (DSHB Hybridoma Product E4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    IF References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    WB References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    IHC References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    IP References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    ELISA References
    FB References

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Epitope Map References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    All References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Inhibition of the histone demethylase Kdm5b promotes neurogenesis and derepresses Reln (reelin) in neural stem cells from the adult subventricular zone of mice.
    Doughty ML
    Molecular biology of the cell 27.4 (2016 Feb 15): 627-39.

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