E4

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SKU: E4

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: E4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Reln
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_1157891 
Uniprot ID: Q60841 
RRID:  
Entrez Gene ID: 19699 
Clonality: Monoclonal
Immunogen: Protein H domain N-terminus
Clone:
Immunogen Sequence: Synthetic peptide-BSA conjugate
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Reelin
Epitope Location or Sequence: The E4 epitope lies between a.a. 164-245
Alternate Antigen Name:
Deposit Date: 7/2/2008
Antigen Molecular Weight: 387.5 kDa
Depositor: Goffinet, A.
Antigen Sequence:
Depositor Institution: University of Louvain, Belgium
Antigen Species: Mouse
Depositor Notes: E4 was generated using the Edinburgh allele reelin mutant mouse. This antibody recognizes the same epitope as anti-reelin G10.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse, Rodent
Additional Information: RRID:AB_1157891
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
E4 was deposited to the DSHB by Goffinet, A. (DSHB Hybridoma Product E4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    IF References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    WB References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    IHC References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    IP References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    ELISA References
    FB References

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Epitope Map References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    All References

    A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice.
    Lambert de Rouvroit C
    Journal of neuroscience methods 82.1 (1998 Jul 1): 17-24.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Inhibition of the histone demethylase Kdm5b promotes neurogenesis and derepresses Reln (reelin) in neural stem cells from the adult subventricular zone of mice.
    Doughty ML
    Molecular biology of the cell 27.4 (2016 Feb 15): 627-39.

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