R4B

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SKU: R4B

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DSHB Data Sheet

Catalog Fields

Product Name/ID: R4B
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Reln
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_1157892 
Uniprot ID: Q60841 
RRID:  
Entrez Gene ID: 19699 
Clonality: Monoclonal
Immunogen: Reelin protein domain a.a. 1796-2115
Clone:
Immunogen Sequence: Synthetic peptide-BSA conjugate
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Reelin
Epitope Location or Sequence: Central region a.a. 1810-1825
Alternate Antigen Name:
Deposit Date: 7/2/2008
Antigen Molecular Weight: 387.5 kDa
Depositor: Goffinet, A.
Antigen Sequence:
Depositor Institution: University of Louvain, Belgium
Antigen Species: Mouse
Depositor Notes: R4B was generated in Reln-Orl mutant mice.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rodent
Additional Information: RRID:AB_1157892
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
R4B was deposited to the DSHB by Goffinet, A. (DSHB Hybridoma Product R4B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    IF References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    WB References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Systematic evaluation of candidate ligands regulating ectodomain shedding of amyloid precursor protein.
    Selkoe DJ
    Biochemistry 52.19 (2013 May 14): 3264-77.

    Seizure-Induced Motility of Differentiated Dentate Granule Cells Is Prevented by the Central Reelin Fragment.
    Haas CA
    Frontiers in cellular neuroscience 10. (2016): 183.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Functional importance of covalent homodimer of reelin protein linked via its central region.
    Takagi J
    The Journal of biological chemistry 286.40 (2011 Oct 7): 35247-56.

    IHC References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Filamin a regulates neural progenitor proliferation and cortical size through Wee1-dependent Cdk1 phosphorylation.
    Sheen VL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.22 (2012 May 30): 7672-84.

    An outbreak of hand, foot, and mouth disease in Singapore.
    Chew SE
    Bulletin of the World Health Organization 60.6 (1982): 965-9.

    IP References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Systematic evaluation of candidate ligands regulating ectodomain shedding of amyloid precursor protein.
    Selkoe DJ
    Biochemistry 52.19 (2013 May 14): 3264-77.

    ELISA References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Inhibition of the histone demethylase Kdm5b promotes neurogenesis and derepresses Reln (reelin) in neural stem cells from the adult subventricular zone of mice.
    Doughty ML
    Molecular biology of the cell 27.4 (2016 Feb 15): 627-39.

    S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T cells.
    Garshasbi M
    Cellular and molecular biology (Noisy-le-Grand, France) 65.6 (2019 Jul 31): 12-16.

    FFPE References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Filamin a regulates neural progenitor proliferation and cortical size through Wee1-dependent Cdk1 phosphorylation.
    Sheen VL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.22 (2012 May 30): 7672-84.

    FB References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Epitope Map References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    All References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Filamin a regulates neural progenitor proliferation and cortical size through Wee1-dependent Cdk1 phosphorylation.
    Sheen VL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.22 (2012 May 30): 7672-84.

    An outbreak of hand, foot, and mouth disease in Singapore.
    Chew SE
    Bulletin of the World Health Organization 60.6 (1982): 965-9.

    Systematic evaluation of candidate ligands regulating ectodomain shedding of amyloid precursor protein.
    Selkoe DJ
    Biochemistry 52.19 (2013 May 14): 3264-77.

    Seizure-Induced Motility of Differentiated Dentate Granule Cells Is Prevented by the Central Reelin Fragment.
    Haas CA
    Frontiers in cellular neuroscience 10. (2016): 183.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Functional importance of covalent homodimer of reelin protein linked via its central region.
    Takagi J
    The Journal of biological chemistry 286.40 (2011 Oct 7): 35247-56.

    Inhibition of the histone demethylase Kdm5b promotes neurogenesis and derepresses Reln (reelin) in neural stem cells from the adult subventricular zone of mice.
    Doughty ML
    Molecular biology of the cell 27.4 (2016 Feb 15): 627-39.

    S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T cells.
    Garshasbi M
    Cellular and molecular biology (Noisy-le-Grand, France) 65.6 (2019 Jul 31): 12-16.

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