R4B

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$40.00
SKU: R4B
View product citations for antibody R4B on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Reelin
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Goffinet, A.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Louvain, Belgium
Positive Tested Species Reactivity: Human, Mouse, Rodent
Depositors Notes: R4B was generated in Reln-Orl mutant mice.
Antigen Molecular Weight: 387.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Reln
Immunogen: Reelin protein domain a.a. 1796-2115
Alternate Gene Names: Rl
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: Central region a.a. 1810-1825
Uniprot ID: Q60841 
Immunogen Sequence: Synthetic peptide-BSA conjugate
Entrez Gene ID: 19699 
Additional Characterization:  
Antibody Registry ID: AB_1157892 
Additional Information: RRID:AB_1157892
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
R4B was deposited to the DSHB by Goffinet, A. (DSHB Hybridoma Product R4B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    IF References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    WB References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Systematic evaluation of candidate ligands regulating ectodomain shedding of amyloid precursor protein.
    Selkoe DJ
    Biochemistry 52.19 (2013 May 14): 3264-77.

    Seizure-Induced Motility of Differentiated Dentate Granule Cells Is Prevented by the Central Reelin Fragment.
    Haas CA
    Frontiers in cellular neuroscience 10. (2016): 183.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Functional importance of covalent homodimer of reelin protein linked via its central region.
    Takagi J
    The Journal of biological chemistry 286.40 (2011 Oct 7): 35247-56.

    IHC References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Filamin a regulates neural progenitor proliferation and cortical size through Wee1-dependent Cdk1 phosphorylation.
    Sheen VL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.22 (2012 May 30): 7672-84.

    An outbreak of hand, foot, and mouth disease in Singapore.
    Chew SE
    Bulletin of the World Health Organization 60.6 (1982): 965-9.

    IP References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Systematic evaluation of candidate ligands regulating ectodomain shedding of amyloid precursor protein.
    Selkoe DJ
    Biochemistry 52.19 (2013 May 14): 3264-77.

    ELISA References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Inhibition of the histone demethylase Kdm5b promotes neurogenesis and derepresses Reln (reelin) in neural stem cells from the adult subventricular zone of mice.
    Doughty ML
    Molecular biology of the cell 27.4 (2016 Feb 15): 627-39.

    S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T cells.
    Garshasbi M
    Cellular and molecular biology (Noisy-le-Grand, France) 65.6 (2019 Jul 31): 12-16.

    FFPE References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Filamin a regulates neural progenitor proliferation and cortical size through Wee1-dependent Cdk1 phosphorylation.
    Sheen VL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.22 (2012 May 30): 7672-84.

    FB References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Epitope Map References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    All References

    Processing of Reelin by embryonic neurons is important for function in tissue but not in dissociated cultured neurons.
    Goffinet AM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.16 (2007 Apr 18): 4243-52.

    Filamin a regulates neural progenitor proliferation and cortical size through Wee1-dependent Cdk1 phosphorylation.
    Sheen VL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.22 (2012 May 30): 7672-84.

    An outbreak of hand, foot, and mouth disease in Singapore.
    Chew SE
    Bulletin of the World Health Organization 60.6 (1982): 965-9.

    Systematic evaluation of candidate ligands regulating ectodomain shedding of amyloid precursor protein.
    Selkoe DJ
    Biochemistry 52.19 (2013 May 14): 3264-77.

    Seizure-Induced Motility of Differentiated Dentate Granule Cells Is Prevented by the Central Reelin Fragment.
    Haas CA
    Frontiers in cellular neuroscience 10. (2016): 183.

    The N-terminal region of reelin regulates postnatal dendritic maturation of cortical pyramidal neurons.
    van Hooft JA
    Proceedings of the National Academy of Sciences of the United States of America 106.17 (2009 Apr 28): 7227-32.

    Functional importance of covalent homodimer of reelin protein linked via its central region.
    Takagi J
    The Journal of biological chemistry 286.40 (2011 Oct 7): 35247-56.

    Inhibition of the histone demethylase Kdm5b promotes neurogenesis and derepresses Reln (reelin) in neural stem cells from the adult subventricular zone of mice.
    Doughty ML
    Molecular biology of the cell 27.4 (2016 Feb 15): 627-39.

    S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T cells.
    Garshasbi M
    Cellular and molecular biology (Noisy-le-Grand, France) 65.6 (2019 Jul 31): 12-16.

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