F5H7 anti-Fasciclin I

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$40.00
SKU: F5H7 anti-Fasciclin I
View product citations for antibody F5H7 anti-Fasciclin I on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: fasciclin I (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: It is important to use a low concentration of saponin (0.02%) instead of triton for immunohisto- chemistry.
Antigen Molecular Weight: Prediced: 73 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Fas1
Immunogen: recombinant, protein A fusion protein
Alternate Gene Names: FasI; CG6588
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P10674 
Immunogen Sequence: Total protein
Entrez Gene ID: 42025 
Additional Characterization:  
Antibody Registry ID: AB_528233 
Additional Information:
Recommended Applications: ELISA, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F5H7 anti-Fasciclin I was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product F5H7 anti-Fasciclin I)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • WB References
  • ELISA References
  • All References
  • Initial Publication
    WB References
    ELISA References
    All References

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