HDC U33
Catalog Fields
Antigen: Headcase
Hybridoma Cells Available: Yes
Antigen Species: drosophila
Depositor: White, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Physiology, Development and Neuroscience, University of Cambridge, UK
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 71 kDa (short) and 117 kDa (full length); Apparent: 70 kDa (short) and 120 kDa (full length)
Predicted Species Reactivity:
Gene: hdc
Immunogen: Recombinant headcase protein (aa 44-462)
Alternate Gene Names: CG15532, psg12
Alternate Antibody Name: U33.7
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: aa 44-462
Uniprot ID: Q9N2M8
Immunogen Sequence: Partial protein
Entrez Gene ID: 43604
Antibody Registry ID: AB_10659722
Additional Information: U33 recognizes the short and full length forms of HDC, but will not recognize the 201 aa B isoform.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HDC U33 was deposited to the DSHB by White, R. (DSHB Hybridoma Product HDC U33)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
9 References
Initial Publication
headcase, an imaginal specific gene required for adult morphogenesis in Drosophila melanogaster.
White RA
Development (Cambridge, England) 121.12 (1995 Dec): 4149-60.