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View product citations for antibody HDC U33 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Headcase
Hybridoma Cells Available: Yes
Antigen Species: drosophila
Depositor: White, R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Cambridge
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 71 kDa (short) and 117 kDa (full length); Apparent: 70 kDa (short) and 120 kDa (full length)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: hdc
Immunogen: Recombinant headcase protein (aa 44-462)
Alternate Gene Names: CG15532, psg12
Alternate Antibody Name: U33.7
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NSO
Epitope Location or Sequence: aa 44-462
Uniprot ID: Q9N2M8 
Immunogen Sequence: Partial protein
Entrez Gene ID: 43604 
Additional Characterization:  
Antibody Registry ID: AB_10659722 
Additional Information: U33 recognizes the short and full length forms of HDC, but will not recognize the 201 aa B isoform.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HDC U33 was deposited to the DSHB by White, R. (DSHB Hybridoma Product HDC U33)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    headcase, an imaginal specific gene required for adult morphogenesis in Drosophila melanogaster.
    White RA
    Development (Cambridge, England) 121.12 (1995 Dec): 4149-60.

    IF References

    An interaction screen identifies headcase as a regulator of large-scale pruning.
    Williams DW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.48 (2012 Nov 28): 17086-96.

    Specification of differentiated adult progenitors via inhibition of endocycle entry in the Drosophila trachea.
    Casanova J
    Cell reports 9.3 (2014 Nov 6): 859-65.

    Dpp and Notch specify the fusion cell fate in the dorsal branches of the Drosophila trachea.
    Samakovlis C
    Mechanisms of development 87.1-2 (1999 Sep): 153-63.

    Unkempt is negatively regulated by mTOR and uncouples neuronal differentiation from growth control.
    Bateman JM
    PLoS genetics 10.9 (2014 Sep): e1004624.

    members only encodes a Drosophila nucleoporin required for rel protein import and immune response activation.
    Samakovlis C
    Genes & development 14.15 (2000 Aug 1): 1945-57.

    WB References
    IHC References
    Epitope Map References

    headcase, an imaginal specific gene required for adult morphogenesis in Drosophila melanogaster.
    White RA
    Development (Cambridge, England) 121.12 (1995 Dec): 4149-60.

    All References

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