JIE7

(0) No Reviews yet
$40.00
SKU: JIE7
View product citations for antibody JIE7 on CiteAb

In Stock

Available: 115

DSHB Data Sheet

Catalog Fields

Antigen: beta-galactosidase
Hybridoma Cells Available: Yes
Antigen Species: E. coli
Depositor: Mason, T.L. / Partaledis, J.A.
Isotype: MIgG2c, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Massachusetts Amherst
Positive Tested Species Reactivity: E. coli
Depositors Notes: This antigen is also known as lactase or lacZ.
Antigen Molecular Weight: 119 kda
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: lacZ
Immunogen: E. coli beta-galactosidase
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: X63.Ag8.653
Epitope Location or Sequence:
Uniprot ID: P00722 
Immunogen Sequence: Purified protein (Sigma cat # G5635)
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528101 
Additional Information: RRID:AB_528101
Recommended Applications:  
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JIE7 was deposited to the DSHB by Mason, T.L. / Partaledis, J.A. (DSHB Hybridoma Product JIE7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • IF References

    Identification of the lacZ insertion site and beta-galactosidase expression in transgenic chickens.
    Petitte JN
    Cell and tissue research 324.1 (2006 Apr): 41-53.

    The depletion of skeletal muscle satellite cells with age is concomitant with reduced capacity of single progenitors to produce reserve progeny.
    Yablonka-Reuveni Z
    Developmental biology 340.2 (2010 Apr 15): 330-43.

    Myogenic reprogramming of retina-derived cells following their spontaneous fusion with myotubes.
    Yablonka-Reuveni Z
    Developmental biology 311.2 (2007 Nov 15): 449-63.

    Daughterless homodimer synergizes with Eyeless to induce Atonal expression and retinal neuron differentiation.
    Du W
    Developmental biology 392.2 (2014 Aug 15): 256-65.

    Frizzled10 mediates WNT1 and WNT3A signaling in the dorsal spinal cord of the developing chick embryo.
    Burrus LW
    Developmental dynamics : an official publication of the American Association of Anatomists 243.6 (2014 Jun): 833-843.

    WB References
    IHC References

    Identification of the lacZ insertion site and beta-galactosidase expression in transgenic chickens.
    Petitte JN
    Cell and tissue research 324.1 (2006 Apr): 41-53.

    The PTK7-related transmembrane proteins off-track and off-track 2 are co-receptors for Drosophila Wnt2 required for male fertility.
    Wodarz A
    PLoS genetics 10.7 (2014 Jul): e1004443.

    Apical-basal polarity in Drosophila neuroblasts is independent of vesicular trafficking.
    Wodarz A
    Molecular biology of the cell 22.22 (2011 Nov): 4373-9.

    A novel Pzg-NURF complex regulates Notch target gene activity.
    Nagel AC
    Molecular biology of the cell 21.19 (2010 Oct 1): 3443-8.

    The Sno oncogene antagonizes Wingless signaling during wing development in Drosophila.
    Newfeld SJ
    PloS one 5.7 (2010 Jul 16): e11619.

    The glypican Dally is required in the niche for the maintenance of germline stem cells and short-range BMP signaling in the Drosophila ovary.
    Wang Z
    Development (Cambridge, England) 136.21 (2009 Nov): 3627-35.

    Regulation of apoptosis of rbf mutant cells during Drosophila development.
    Du W
    Developmental biology 326.2 (2009 Feb 15): 347-56.

    Mutation of the Apc1 homologue shattered disrupts normal eye development by disrupting G1 cell cycle arrest and progression through mitosis.
    Du W
    Developmental biology 309.2 (2007 Sep 15): 222-35.

    Nef induces apoptosis by activating JNK signaling pathway and inhibits NF-kappaB-dependent immune responses in Drosophila.
    Chung J
    Journal of cell science 118.Pt 9 (2005 May 1): 1851-9.

    Dynamic patterns of retinoic acid synthesis and response in the developing mammalian heart.
    Rosenthal N
    Developmental biology 199.1 (1998 Jul 1): 55-71.

    Drosophila Cyclin G Is a Regulator of the Notch Signalling Pathway during Wing Development.
    Preiss A
    PloS one 11.3 (2016): e0151477.

    Apical-basal polarity in Drosophila neuroblasts is independent of vesicular trafficking.
    Wodarz A
    Molecular biology of the cell 22.22 (2011 Nov): 4373-9.

    FFPE References
    All References

    Identification of the lacZ insertion site and beta-galactosidase expression in transgenic chickens.
    Petitte JN
    Cell and tissue research 324.1 (2006 Apr): 41-53.

    The PTK7-related transmembrane proteins off-track and off-track 2 are co-receptors for Drosophila Wnt2 required for male fertility.
    Wodarz A
    PLoS genetics 10.7 (2014 Jul): e1004443.

    Apical-basal polarity in Drosophila neuroblasts is independent of vesicular trafficking.
    Wodarz A
    Molecular biology of the cell 22.22 (2011 Nov): 4373-9.

    A novel Pzg-NURF complex regulates Notch target gene activity.
    Nagel AC
    Molecular biology of the cell 21.19 (2010 Oct 1): 3443-8.

    The Sno oncogene antagonizes Wingless signaling during wing development in Drosophila.
    Newfeld SJ
    PloS one 5.7 (2010 Jul 16): e11619.

    The glypican Dally is required in the niche for the maintenance of germline stem cells and short-range BMP signaling in the Drosophila ovary.
    Wang Z
    Development (Cambridge, England) 136.21 (2009 Nov): 3627-35.

    Regulation of apoptosis of rbf mutant cells during Drosophila development.
    Du W
    Developmental biology 326.2 (2009 Feb 15): 347-56.

    Mutation of the Apc1 homologue shattered disrupts normal eye development by disrupting G1 cell cycle arrest and progression through mitosis.
    Du W
    Developmental biology 309.2 (2007 Sep 15): 222-35.

    Nef induces apoptosis by activating JNK signaling pathway and inhibits NF-kappaB-dependent immune responses in Drosophila.
    Chung J
    Journal of cell science 118.Pt 9 (2005 May 1): 1851-9.

    Dynamic patterns of retinoic acid synthesis and response in the developing mammalian heart.
    Rosenthal N
    Developmental biology 199.1 (1998 Jul 1): 55-71.

    Drosophila Cyclin G Is a Regulator of the Notch Signalling Pathway during Wing Development.
    Preiss A
    PloS one 11.3 (2016): e0151477.

    The depletion of skeletal muscle satellite cells with age is concomitant with reduced capacity of single progenitors to produce reserve progeny.
    Yablonka-Reuveni Z
    Developmental biology 340.2 (2010 Apr 15): 330-43.

    Myogenic reprogramming of retina-derived cells following their spontaneous fusion with myotubes.
    Yablonka-Reuveni Z
    Developmental biology 311.2 (2007 Nov 15): 449-63.

    Daughterless homodimer synergizes with Eyeless to induce Atonal expression and retinal neuron differentiation.
    Du W
    Developmental biology 392.2 (2014 Aug 15): 256-65.

    Frizzled10 mediates WNT1 and WNT3A signaling in the dorsal spinal cord of the developing chick embryo.
    Burrus LW
    Developmental dynamics : an official publication of the American Association of Anatomists 243.6 (2014 Jun): 833-843.

    Calpains mediate integrin attachment complex maintenance of adult muscle in Caenorhabditis elegans.
    Szewczyk NJ
    PLoS genetics 8.1 (2012 Jan): e1002471.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review