JIE7
Catalog Fields
Clone ID/Product Name: JIE7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: lacZ
Ab Isotype: MIgG2c, kappa light chain
Gene Name:
Clonality: Monoclonal
Immunogen: E. coli beta-galactosidase
Clone:
Immunogen Sequence: Purified protein (Sigma cat # G5635)
Myeloma Strain: X63.Ag8.653
Epitope Mapped: No
Antigen Name: beta-galactosidase
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/1/1989
Antigen Molecular Weight: 119 kda
Depositor: Mason, T.L. / Partaledis, J.A.
Antigen Sequence:
Depositor Institution: University of Massachusetts Amherst
Antigen Species: E. coli
Depositor Notes: This antigen is also known as lactase or lacZ.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: E. coli
Additional Information: RRID:AB_528101
Recommended Applications:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JIE7 was deposited to the DSHB by Mason, T.L. / Partaledis, J.A. (DSHB Hybridoma Product JIE7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
17 References