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DSHB Data Sheet

Catalog Fields

Product Name/ID: NP1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: dap
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_10805540 
Uniprot ID: P91668 
Entrez Gene ID: 36001 
Clonality: Monoclonal
Immunogen: Recombinant Histidine-tagged Dap protein
Immunogen Sequence: Full length protein
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Dacapo
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/8/2010
Antigen Molecular Weight: Predicted: 27 kDa
Depositor: Hariharan, I.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Dacapo is a homolog of the cyclin-dependent kinase inhibitor p21/p27.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
NP1 was deposited to the DSHB by Hariharan, I. (DSHB Hybridoma Product NP1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Expression of the cyclin-dependent kinase inhibitor Dacapo is regulated by cyclin E.
    Hariharan IK
    Mechanisms of development 97.1-2 (2000 Oct): 73-83.

    IF References
    WB References

    RNA helicase Belle (DDX3) is essential for male germline stem cell maintenance and division in Drosophila.
    Olenina LV
    Biochimica et biophysica acta 1863.6 Pt A (2016 Jun): 1093-105.

    IHC References

    Expression of the cyclin-dependent kinase inhibitor Dacapo is regulated by cyclin E.
    Hariharan IK
    Mechanisms of development 97.1-2 (2000 Oct): 73-83.

    All References

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