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DSHB Data Sheet

Catalog Fields

Antigen: Dacapo
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Hariharan, I.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 27 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: dap
Immunogen: Recombinant Histidine-tagged Dap protein
Alternate Gene Names: CG1772, Cdi4, p21, p27
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P91668 
Immunogen Sequence: Full length protein
Entrez Gene ID: 36001 
Additional Characterization:  
Antibody Registry ID: AB_10805540 
Additional Information: Dacapo is a homolog of the cyclin-dependent kinase inhibitor p21/p27.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
NP1 was deposited to the DSHB by Hariharan, I. (DSHB Hybridoma Product NP1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Expression of the cyclin-dependent kinase inhibitor Dacapo is regulated by cyclin E.
    Hariharan IK
    Mechanisms of development 97.1-2 (2000 Oct): 73-83.

    IF References
    WB References

    RNA helicase Belle (DDX3) is essential for male germline stem cell maintenance and division in Drosophila.
    Olenina LV
    Biochimica et biophysica acta 1863.6 Pt A (2016 Jun): 1093-105.

    IHC References

    Expression of the cyclin-dependent kinase inhibitor Dacapo is regulated by cyclin E.
    Hariharan IK
    Mechanisms of development 97.1-2 (2000 Oct): 73-83.

    All References

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