A12

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$40.00
SKU: A12
View product citations for antibody A12 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Cyclin A
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Lehner, C.F.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Bayreuth
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 56 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: CycA
Immunogen: Cyclin A (a.a 6-491); recombinant
Alternate Gene Names: Cyc A; cycA; CYCA; dCycA; DmcycA; Dmel\CG5940;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P14785 
Immunogen Sequence:
Entrez Gene ID: 39340 
Additional Characterization:
Antibody Registry ID: AB_528188 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A12 was deposited to the DSHB by Lehner, C.F. (DSHB Hybridoma Product A12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References

    Expression and function of Drosophila cyclin A during embryonic cell cycle progression.
    O'Farrell PH
    Cell 56.6 (1989 Mar 24): 957-68.

    Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3.
    Lehner CF
    The EMBO journal 14.19 (1995 Oct 2): 4827-38.

    Induction of endocycles represses apoptosis independently of differentiation and predisposes cells to genome instability.
    Calvi BR
    Development (Cambridge, England) 141.1 (2014 Jan): 112-23.

    Control of DNA replication and chromosome ploidy by geminin and cyclin A.
    Zhang H
    Molecular and cellular biology 22.6 (2002 Mar): 1868-80.

    Aberrant monomethylation of histone H4 lysine 20 activates the DNA damage checkpoint in Drosophila melanogaster.
    Steward R
    The Journal of cell biology 176.2 (2007 Jan 15): 155-62.

    Regulation of cyclin A localization downstream of Par-1 function is critical for the centrosome orientation checkpoint in Drosophila male germline stem cells.
    Yamashita YM
    Developmental biology 361.1 (2012 Jan 1): 57-67.

    Asunder is a critical regulator of dynein-dynactin localization during Drosophila spermatogenesis.
    Lee LA
    Molecular biology of the cell 20.11 (2009 Jun): 2709-21.

    Bruno inhibits the expression of mitotic cyclins during the prophase I meiotic arrest of Drosophila oocytes.
    Lilly MA
    Developmental cell 10.1 (2006 Jan): 127-35.

    PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigen.
    Megraw TL
    Oncogene 27.49 (2008 Oct 23): 6334-46.

    Hypoxia induces major effects on cell cycle kinetics and protein expression in Drosophila melanogaster embryos.
    Haddad GG
    American journal of physiology. Regulatory, integrative and comparative physiology 288.2 (2005 Feb): R511-21.

    dFMRP and Caprin, translational regulators of synaptic plasticity, control the cell cycle at the Drosophila mid-blastula transition.
    Sisson JC
    Development (Cambridge, England) 137.24 (2010 Dec): 4201-9.

    IHC References

    Expression and function of Drosophila cyclin A during embryonic cell cycle progression.
    O'Farrell PH
    Cell 56.6 (1989 Mar 24): 957-68.

    Polycomb group-dependent Cyclin A repression in Drosophila.
    Cavalli G
    Genes and development 20.4 (2006 Feb 15): 501-13.

    gigas, a Drosophila homolog of tuberous sclerosis gene product-2, regulates the cell cycle.
    Rubin GM
    Cell 96.4 (1999 Feb 19): 529-39.

    Regulation of cyclin A localization downstream of Par-1 function is critical for the centrosome orientation checkpoint in Drosophila male germline stem cells.
    Yamashita YM
    Developmental biology 361.1 (2012 Jan 1): 57-67.

    APC/CFzr/Cdh1 promotes cell cycle progression during the Drosophila endocycle.
    Lilly MA
    Development (Cambridge, England) 135.8 (2008 Apr): 1451-61.

    The Drosophila tumor suppressors Expanded and Merlin differentially regulate cell cycle exit, apoptosis, and Wingless signaling.
    Hariharan IK
    Developmental biology 304.1 (2007 Apr 1): 102-15.

    Bruno inhibits the expression of mitotic cyclins during the prophase I meiotic arrest of Drosophila oocytes.
    Lilly MA
    Developmental cell 10.1 (2006 Jan): 127-35.

    Barrier-to-autointegration factor plays crucial roles in cell cycle progression and nuclear organization in Drosophila.
    Nishida Y
    Journal of cell science 116.Pt 18 (2003 Sep 15): 3811-23.

    Smoothened, thickveins and the genetic control of cell cycle and cell fate in the developing Drosophila eye.
    Moses K
    Mechanisms of development 123.2 (2006 Feb): 151-65.

    Characterization of Drosophila mini-me, a gene required for cell proliferation and survival.
    Moses K
    Genetics 173.2 (2006 Jun): 793-808.

    The gang of four gene regulates growth and patterning of the developing Drosophila eye.
    Moberg K
    Fly 4.2 (2010 Apr-Jun): 104-16.

    IP References

    Control of DNA replication and chromosome ploidy by geminin and cyclin A.
    Zhang H
    Molecular and cellular biology 22.6 (2002 Mar): 1868-80.

    Polycomb group-dependent Cyclin A repression in Drosophila.
    Cavalli G
    Genes and development 20.4 (2006 Feb 15): 501-13.

    Six2 and Wnt regulate self-renewal and commitment of nephron progenitors through shared gene regulatory networks.
    McMahon AP
    Developmental cell 23.3 (2012 Sep 11): 637-51.

    All References

    Expression and function of Drosophila cyclin A during embryonic cell cycle progression.
    O'Farrell PH
    Cell 56.6 (1989 Mar 24): 957-68.

    Polycomb group-dependent Cyclin A repression in Drosophila.
    Cavalli G
    Genes and development 20.4 (2006 Feb 15): 501-13.

    gigas, a Drosophila homolog of tuberous sclerosis gene product-2, regulates the cell cycle.
    Rubin GM
    Cell 96.4 (1999 Feb 19): 529-39.

    Regulation of cyclin A localization downstream of Par-1 function is critical for the centrosome orientation checkpoint in Drosophila male germline stem cells.
    Yamashita YM
    Developmental biology 361.1 (2012 Jan 1): 57-67.

    APC/CFzr/Cdh1 promotes cell cycle progression during the Drosophila endocycle.
    Lilly MA
    Development (Cambridge, England) 135.8 (2008 Apr): 1451-61.

    The Drosophila tumor suppressors Expanded and Merlin differentially regulate cell cycle exit, apoptosis, and Wingless signaling.
    Hariharan IK
    Developmental biology 304.1 (2007 Apr 1): 102-15.

    Bruno inhibits the expression of mitotic cyclins during the prophase I meiotic arrest of Drosophila oocytes.
    Lilly MA
    Developmental cell 10.1 (2006 Jan): 127-35.

    Barrier-to-autointegration factor plays crucial roles in cell cycle progression and nuclear organization in Drosophila.
    Nishida Y
    Journal of cell science 116.Pt 18 (2003 Sep 15): 3811-23.

    Smoothened, thickveins and the genetic control of cell cycle and cell fate in the developing Drosophila eye.
    Moses K
    Mechanisms of development 123.2 (2006 Feb): 151-65.

    Characterization of Drosophila mini-me, a gene required for cell proliferation and survival.
    Moses K
    Genetics 173.2 (2006 Jun): 793-808.

    The gang of four gene regulates growth and patterning of the developing Drosophila eye.
    Moberg K
    Fly 4.2 (2010 Apr-Jun): 104-16.

    Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3.
    Lehner CF
    The EMBO journal 14.19 (1995 Oct 2): 4827-38.

    Asunder is a critical regulator of dynein-dynactin localization during Drosophila spermatogenesis.
    Lee LA
    Molecular biology of the cell 20.11 (2009 Jun): 2709-21.

    Zfrp8, the Drosophila ortholog of PDCD2, functions in lymph gland development and controls cell proliferation.
    Steward R
    Development (Cambridge, England) 134.13 (2007 Jul): 2387-96.

    Two components of the Myb complex, DMyb and Mip130, are specifically associated with euchromatin and degraded during prometaphase throughout development.
    Katzen AL
    Mechanisms of development 125.7 (2008 Jul): 646-61.

    Induction of endocycles represses apoptosis independently of differentiation and predisposes cells to genome instability.
    Calvi BR
    Development (Cambridge, England) 141.1 (2014 Jan): 112-23.

    Control of DNA replication and chromosome ploidy by geminin and cyclin A.
    Zhang H
    Molecular and cellular biology 22.6 (2002 Mar): 1868-80.

    Aberrant monomethylation of histone H4 lysine 20 activates the DNA damage checkpoint in Drosophila melanogaster.
    Steward R
    The Journal of cell biology 176.2 (2007 Jan 15): 155-62.

    PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigen.
    Megraw TL
    Oncogene 27.49 (2008 Oct 23): 6334-46.

    Hypoxia induces major effects on cell cycle kinetics and protein expression in Drosophila melanogaster embryos.
    Haddad GG
    American journal of physiology. Regulatory, integrative and comparative physiology 288.2 (2005 Feb): R511-21.

    dFMRP and Caprin, translational regulators of synaptic plasticity, control the cell cycle at the Drosophila mid-blastula transition.
    Sisson JC
    Development (Cambridge, England) 137.24 (2010 Dec): 4201-9.

    Six2 and Wnt regulate self-renewal and commitment of nephron progenitors through shared gene regulatory networks.
    McMahon AP
    Developmental cell 23.3 (2012 Sep 11): 637-51.

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