Nub 2D4

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SKU: Nub 2D4

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Nub 2D4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: nub
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2722119 
Uniprot ID: P31368 
Entrez Gene ID: 34669 
Clonality: Monoclonal
Immunogen: Recombinant Artermia sequence
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: nubbin (Pdm)
Epitope Location or Sequence: Epitope lies within the homeodomain
Alternate Antigen Name:
Deposit Date: 4/27/2017
Antigen Molecular Weight: 65.2 kDa
Depositor: Averof, Michalis
Antigen Sequence:
Depositor Institution: Institut de Genomique Fonctionnelle de Lyon
Antigen Species: Artemia
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Artemia, Crayfish, Crustacean, Drosophila, Horseshoe crab, Spider
Additional Information: FBgn0085424 RRID:AB_2722119
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Nub 2D4 was deposited to the DSHB by Averof, Michalis (DSHB Hybridoma Product Nub 2D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Evolutionary origin of insect wings from ancestral gills.
    Cohen SM
    Nature 385.6617 (1997 Feb 13): 627-30.

    IF References
    IHC References
    Epitope Map References
    All References

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