Nub 2D4

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SKU: Nub 2D4-s
View product citations for antibody Nub 2D4 on CiteAb

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Available: 170

DSHB Data Sheet

Catalog Fields

Antigen: nubbin (Pdm)
Hybridoma Cells Available: Yes
Antigen Species: Artemia
Depositor: Averof, Michalis
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institut de Génomique Fonctionnelle de Lyon
Positive Tested Species Reactivity: Artemia, Crayfish, Crustacean, Drosophila, Horseshoe crab, Spider
Depositors Notes:
Antigen Molecular Weight: 65.2 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: nub
Immunogen: Recombinant Artermia sequence
Alternate Gene Names: pdm1/OCT1/POU-19/TWN
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: Epitope lies within the homeodomain
Uniprot ID: P31368 
Immunogen Sequence:
Entrez Gene ID: 34669 
Additional Characterization:  
Antibody Registry ID: AB_2722119 
Additional Information: FBgn0085424 RRID:AB_2722119
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Nub 2D4 was deposited to the DSHB by Averof, Michalis (DSHB Hybridoma Product Nub 2D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Evolutionary origin of insect wings from ancestral gills.
    Cohen SM
    Nature 385.6617 (1997 Feb 13): 627-30.

    IF References
    IHC References
    Epitope Map References
    All References

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