Nub 2D4

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SKU: Nub 2D4-s
View product citations for antibody Nub 2D4 on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: nubbin (Pdm)
Hybridoma Cells Available: Yes
Antigen Species: Artemia
Depositor: Averof, Michalis
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institut de Génomique Fonctionnelle de Lyon
Positive Tested Species Reactivity: Artemia, Crayfish, Crustacean, Drosophila, Horseshoe crab, Spider
Depositors Notes:
Antigen Molecular Weight: 65.2 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: nub
Immunogen: Recombinant Artermia sequence
Alternate Gene Names: pdm1/OCT1/POU-19/TWN
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: Epitope lies within the homeodomain
Uniprot ID: P31368 
Immunogen Sequence:
Entrez Gene ID: 34669 
Additional Characterization:
Antibody Registry ID: AB_2722119 
Additional Information: FBgn0085424 RRID:AB_2722119
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Nub 2D4 was deposited to the DSHB by Averof, Michalis (DSHB Hybridoma Product Nub 2D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
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