P4D11

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$45.00 to $425.00
SKU: P4D11
View product citations for antibody P4D11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Disabled protein
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Giniger, E.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: NIH (NINDS)
Antigen Molecular Weight: Predicted: 227 and 243 kDa (at least two isoforms); Apparent: around 280 kDa
Depositors Notes: P4D11 is recommended for Immunoprecipitation, and P6E11 is recommended for Immunohistochemistry and Immunoblotting.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant Drosophila Disabled protein (N-terminal 2/3)/His(6)-tagged protein
Gene: Dab
Alternate Antibody Name:
Alternate Gene Names: CG9695
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: Yes
Uniprot ID: P98081 
Epitope Location or Sequence: N-terminal 2/3 of the protein
Entrez Gene ID: 39866 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528200 
Additional Characterization:  
Recommended Applications: ELISA, Immunoprecipitation
Additional Information: P4D11 gives some nonspecific background by Immunohistochemistry (P6E11 gives less background) [PMID 18062953].
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4D11 was deposited to the DSHB by Giniger, E. (DSHB Hybridoma Product P4D11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    IP References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

    ELISA References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    Epitope Map References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    All References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

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