P6E11

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$40.00
SKU: P6E11
View product citations for antibody P6E11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Disabled protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Giniger, E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: NIH (NINDS)
Positive Tested Species Reactivity: Drosophila
Depositors Notes: P4D11 is recommended for Immunoprecipitation, and P6E11 is recommended for Immunohistochemistry and Immunoblotting.
Antigen Molecular Weight: Predicted: 227 and 243 kDa (at least two isoforms); Apparent: around 280 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Dab
Immunogen: Recombinant Drosophila Disabled protein (N-terminal 2/3)/His(6)-tagged protein
Alternate Gene Names: CG9695
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: N-terminal 2/3 of the protein
Uniprot ID: P98081 
Immunogen Sequence: Partial protein
Entrez Gene ID: 39866 
Additional Characterization:  
Antibody Registry ID: AB_528201 
Additional Information: P6E11 gives some nonspecific background by Immunohistochemistry but is better than P4D11 [PMID 18062953]. PE611 has been reported to recognize both isoforms reported by Gertler et al (1993; [PMID 7680635]) [PMID 20940230].
Recommended Applications: ELISA, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P6E11 was deposited to the DSHB by Giniger, E. (DSHB Hybridoma Product P6E11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    WB References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

    IHC References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    ELISA References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    Epitope Map References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    All References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

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