P6E11

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SKU: P6E11

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DSHB Data Sheet

Catalog Fields

Product Name/ID: P6E11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Dab
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528201 
Uniprot ID: P98081 
RRID:  
Entrez Gene ID: 39866 
Clonality: Monoclonal
Immunogen: Recombinant Drosophila Disabled protein (N-terminal 2/3)/His(6)-tagged protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: Disabled protein
Epitope Location or Sequence: N-terminal 2/3 of the protein
Alternate Antigen Name:
Deposit Date: 3/12/2004
Antigen Molecular Weight: Predicted: 227 and 243 kDa (at least two isoforms); Apparent: around 280 kDa
Depositor: Giniger, E.
Antigen Sequence:
Depositor Institution: NIH (NINDS)
Antigen Species: Drosophila
Depositor Notes: P4D11 is recommended for Immunoprecipitation, and P6E11 is recommended for Immunohistochemistry and Immunoblotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: P6E11 gives some nonspecific background by Immunohistochemistry but is better than P4D11 [PMID 18062953]. PE611 has been reported to recognize both isoforms reported by Gertler et al (1993; [PMID 7680635]) [PMID 20940230].
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P6E11 was deposited to the DSHB by Giniger, E. (DSHB Hybridoma Product P6E11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    WB References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

    IHC References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    ELISA References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    Epitope Map References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    All References

    Molecular separation of two signaling pathways for the receptor, Notch.
    Giniger E
    Developmental biology 313.2 (2008 Jan 15): 556-67.

    Disabled is a bona fide component of the Abl signaling network.
    Giniger E
    Development (Cambridge, England) 137.21 (2010 Nov): 3719-27.

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