T4

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SKU: T4-s

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Supernatant 1.0 ml

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DSHB Data Sheet

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Clone ID/Product Name: T4

Available to For-Profits: No

Alternate Antibody Name:

Gene Symbol: SLC12A1, SLC12A2

Ab Isotype: MIgG1

Gene Name:

Antibody Registry ID: AB_528406

Uniprot ID: P55011 Q13621

RRID:

Entrez Gene ID: 6557 6558

Clonality: Monoclonal

Immunogen: Recombinant 38kDa peptide fragment of carboxy terminus encoded by cDNA from T84 (human) epithelial Na-K-Cl cotransport protein

Clone:

Immunogen Sequence: human NKCC1 C-terminus (Met902-Ser1212)

Myeloma Strain: P3x63-Ag8.653

Epitope Mapped: Yes

Antigen Name: Na-K-Cl cotransporter

Epitope Location or Sequence: a.a. 902-1212

Alternate Antigen Name: NKCC, Solute carrier family 12 members 1 and 3

Deposit Date: 2/2/1996

Antigen Molecular Weight: Predicted: 131.4kDa Apparent: 145-205kDa

Depositor: Lytle, C. / Forbush III, B.

Antigen Sequence:

Depositor Institution: Yale University

Antigen Species: Human

Depositor Notes: Antigen retrieval (1% SDS for 5 min) required for immunostaining frozen sections. T4 recognizes NKCC1 and NKCC2 on western blots and by IF, but is weak for NKCC2 by IF (PMID: 8572179)

Host Species: mouse

Hybridoma Cells Available (Non-Profit): No

Confirmed Species Reactivity: Avian, Elasmobranch, Fish, Mackerel, Mammal, Rat, Sheep, Xenopus

Additional Information: Paraffin embedment of ethanol fixed tissue.

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

T4 was deposited to the DSHB by Lytle, C. / Forbush III, B. (DSHB Hybridoma Product T4)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

22 References

Initial Publication

IF References

WB References

IHC References

IP References

FFPE References

All References

Initial Publication

Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies.
Forbush B 3rd
The American journal of physiology 269.6 Pt 1 (1995 Dec): C1496-505.

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T4

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