Clone ID / Name: T4
Anti - Na-K-Cl cotransporter
Immunofluorescence image of COS7 cells labeled with DSHB antibody T4 against Na-K-Cl cotransporter (red). PMID 26351455, Fig. 5A.
Catalog Fields
Identity
Clone ID / Name: T4
Alternate Antibody Name: —
Antigen Name: Na-K-Cl cotransporter
Alternate Antigen Name: NKCC, Solute carrier family 12 members 1 and 3
Gene Symbol: —
Gene Name: SLC12A1, SLC12A2
Antigen Sequence: —
Antigen Species: Human
Antigen Mol. Weight: Predicted: 131.4kDa Apparent: 145-205kDa
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: P3x63-Ag8.653
Antibody Registry / RRID: AB_528406
Available to For-Profits: No
Hybridoma Available: No
Immunogen & Epitope
Immunogen: Recombinant 38kDa peptide fragment of carboxy terminus encoded by cDNA from T84 (human) epithelial Na-K-Cl cotransport protein
Immunogen Sequence: human NKCC1 C-terminus (Met902-Ser1212)
Epitope Mapped: Yes
Epitope Location / Seq: a.a. 902-1212
Clone: —
Additional Info: Paraffin embedment of ethanol fixed tissue.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Avian, Elasmobranch, Fish, Mackerel, Mammal, Rat, Sheep, Xenopus
Predicted Reactivity: —
Depositor
Depositor Name: C. Lytle / B. Forbush III
Institution: Yale University
Deposit Date: February 2, 1996
Depositor Notes: Antigen retrieval (1% SDS for 5 min) required for immunostaining frozen sections. T4 recognizes NKCC1 and NKCC2 on western blots and by IF, but is weak for NKCC2 by IF (PMID: 8572179)
Recommended Applications
FFPE
IF
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →