T4

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SKU: T4
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DSHB Data Sheet

Catalog Fields

Antigen: Na-K-Cl cotransporter
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Lytle, C. / Forbush III, B.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale University
Positive Tested Species Reactivity: Avian, Elasmobranch, Fish, Mackerel, Mammal, Rat, Sheep, Xenopus
Depositors Notes: Antigen retrieval (1% SDS for 5 min) required for immunostaining frozen sections. T4 recognizes NKCC1 and NKCC2 on western blots and by IF, but is weak for NKCC2 by IF (PMID: 8572179)
Antigen Molecular Weight: Predicted: 131.4kDa Apparent: 145-205kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SLC12A1, SLC12A2
Immunogen: Recombinant 38kDa peptide fragment of carboxy terminus encoded by cDNA from T84 (human) epithelial Na-K-Cl cotransport protein
Alternate Gene Names: NKCC1 and NKCC2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name: NKCC, Solute carrier family 12 members 1 and 3
Epitope Mapped: Yes
Myeloma Strain: P3x63-Ag8.653
Epitope Location or Sequence: a.a. 902-1212
Uniprot ID: P55011 Q13621 
Immunogen Sequence: human NKCC1 C-terminus (Met902-Ser1212)
Entrez Gene ID: 6557  6558 
Additional Characterization:  
Antibody Registry ID: AB_528406 
Additional Information: Paraffin embedment of ethanol fixed tissue.
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
T4 was deposited to the DSHB by Lytle, C. / Forbush III, B. (DSHB Hybridoma Product T4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • All References
  • Initial Publication

    Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies.
    Forbush B 3rd
    The American journal of physiology 269.6 Pt 1 (1995 Dec): C1496-505.

    IF References

    Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies.
    Forbush B 3rd
    The American journal of physiology 269.6 Pt 1 (1995 Dec): C1496-505.

    Na:K:2Cl cotransporter (NKCC) of intestinal epithelial cells. Surface expression in response to cAMP.
    Madara JL
    The Journal of biological chemistry 271.46 (1996 Nov 15): 28969-76.

    Immunocytochemical studies of the Na-K-Cl cotransporter of shark kidney.
    Forbush B 3rd
    The American journal of physiology 270.6 Pt 2 (1996 Jun): F927-36.

    Letter: Isolated ventricular inversion with situs solitus.
    Wilkinson JL
    British heart journal 37.11 (1975 Nov): 1202.

    Redistribution of immunofluorescence of CFTR anion channel and NKCC cotransporter in chloride cells during adaptation of the killifish Fundulus heteroclitus to sea water.
    Cozzi RR
    The Journal of experimental biology 205.Pt 9 (2002 May): 1265-73.

    Hypotonic shock mediation by p38 MAPK, JNK, PKC, FAK, OSR1 and SPAK in osmosensing chloride secreting cells of killifish opercular epithelium.
    Hoffmann EK
    The Journal of experimental biology 208.Pt 6 (2005 Mar): 1063-77.

    Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and CFTR anion channel.
    Kaneko T
    The Journal of experimental biology 208.Pt 11 (2005 Jun): 2023-36.

    Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater.
    Kaneko T
    The Journal of experimental biology 208.Pt 20 (2005 Oct): 3851-8.

    Variation in salinity tolerance, gill Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and mitochondria-rich cell distribution in three salmonids Salvelinus namaycush, Salvelinus fontinalis and Salmo salar.
    McCormick SD
    The Journal of experimental biology 210.Pt 6 (2007 Mar): 1015-24.

    Focal adhesion kinase and beta1 integrin regulation of Na+, K+, 2Cl- cotransporter in osmosensing ion transporting cells of killifish, Fundulus heteroclitus.
    Cozzi RR
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 150.3 (2008 Jul): 288-300.

    Phorbol 12-myristate 13-acetate-induced endocytosis of the Na-K-2Cl cotransporter in MDCK cells is associated with a clathrin-dependent pathway.
    Matthews JB
    American journal of physiology. Cell physiology 298.1 (2010 Jan): C85-97.

    Identification of a sea urchin Na(+)/K(+)/2Cl(-) cotransporter (NKCC): microfilament-dependent surface expression is mediated by hypotonic shock and cyclic AMP.
    Hofman P
    The Journal of experimental biology 204.Pt 1 (2001 Jan): 147-56.

    Exercise modulates chloride homeostasis after spinal cord injury.
    Houlé JD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.27 (2014 Jul 2): 8976-87.

    Differential expression of Na<sup>+</sup>/K<sup>+</sup>/Cl<sup>-</sup> cotransporter 1 in neurons and glial cells within the superficial spinal dorsal horn of rodents.
    Antal M
    Scientific reports 10.1 (2020 Jul 16): 11715.

    Immunohistochemical characterization and change in location of branchial ionocytes after transfer from freshwater to seawater in the euryhaline obscure puffer, Takifugu obscurus.
    Zhao Z
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.5 (2020 Sep): 585-596.

    WB References

    Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies.
    Forbush B 3rd
    The American journal of physiology 269.6 Pt 1 (1995 Dec): C1496-505.

    Corticosteroids modulate the secretory processes of the rat intrahepatic biliary epithelium.
    Benedetti A
    Gastroenterology 122.4 (2002 Apr): 1058-69.

    Exercise modulates chloride homeostasis after spinal cord injury.
    Houlé JD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.27 (2014 Jul 2): 8976-87.

    Dynamics of Na(+),K(+),2Cl(-) cotransporter and Na(+),K(+)-ATPase expression in the branchial epithelium of brown trout (Salmo trutta) and Atlantic salmon (Salmo salar).
    Cramb G
    The Journal of experimental zoology 293.2 (2002 Jul 1): 106-18.

    Redistribution of immunofluorescence of CFTR anion channel and NKCC cotransporter in chloride cells during adaptation of the killifish Fundulus heteroclitus to sea water.
    Cozzi RR
    The Journal of experimental biology 205.Pt 9 (2002 May): 1265-73.

    Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis).
    Borski RJ
    Journal of experimental zoology. Part A, Comparative experimental biology 301.12 (2004 Dec 1): 979-91.

    Variation in salinity tolerance, gill Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and mitochondria-rich cell distribution in three salmonids Salvelinus namaycush, Salvelinus fontinalis and Salmo salar.
    McCormick SD
    The Journal of experimental biology 210.Pt 6 (2007 Mar): 1015-24.

    Identification of a sea urchin Na(+)/K(+)/2Cl(-) cotransporter (NKCC): microfilament-dependent surface expression is mediated by hypotonic shock and cyclic AMP.
    Hofman P
    The Journal of experimental biology 204.Pt 1 (2001 Jan): 147-56.

    Immunohistochemical characterization and change in location of branchial ionocytes after transfer from freshwater to seawater in the euryhaline obscure puffer, Takifugu obscurus.
    Zhao Z
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.5 (2020 Sep): 585-596.

    Immunological characterization of two types of ionocytes in the inner ear epithelium of Pacific Chub Mackerel (Scomber japonicus).
    Tresguerres M
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.4 (2020 Jul): 419-431.

    IHC References

    IGF-I and branchial IGF receptor expression and localization during salinity acclimation in striped bass.
    Borski RJ
    American journal of physiology. Regulatory, integrative and comparative physiology 292.1 (2007 Jan): R535-43.

    Focal adhesion kinase and beta1 integrin regulation of Na+, K+, 2Cl- cotransporter in osmosensing ion transporting cells of killifish, Fundulus heteroclitus.
    Cozzi RR
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 150.3 (2008 Jul): 288-300.

    Prenatal Testosterone Exposure Alters GABAergic Synaptic Inputs to GnRH and KNDy Neurons in a Sheep Model of Polycystic Ovarian Syndrome.
    Lehman MN
    Endocrinology 160.11 (2019 Nov 1): 2529-2542.

    Immunological characterization of two types of ionocytes in the inner ear epithelium of Pacific Chub Mackerel (Scomber japonicus).
    Tresguerres M
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.4 (2020 Jul): 419-431.

    IP References
    FFPE References
    All References

    IGF-I and branchial IGF receptor expression and localization during salinity acclimation in striped bass.
    Borski RJ
    American journal of physiology. Regulatory, integrative and comparative physiology 292.1 (2007 Jan): R535-43.

    Focal adhesion kinase and beta1 integrin regulation of Na+, K+, 2Cl- cotransporter in osmosensing ion transporting cells of killifish, Fundulus heteroclitus.
    Cozzi RR
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 150.3 (2008 Jul): 288-300.

    Prenatal Testosterone Exposure Alters GABAergic Synaptic Inputs to GnRH and KNDy Neurons in a Sheep Model of Polycystic Ovarian Syndrome.
    Lehman MN
    Endocrinology 160.11 (2019 Nov 1): 2529-2542.

    Immunological characterization of two types of ionocytes in the inner ear epithelium of Pacific Chub Mackerel (Scomber japonicus).
    Tresguerres M
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.4 (2020 Jul): 419-431.

    Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies.
    Forbush B 3rd
    The American journal of physiology 269.6 Pt 1 (1995 Dec): C1496-505.

    Na:K:2Cl cotransporter (NKCC) of intestinal epithelial cells. Surface expression in response to cAMP.
    Madara JL
    The Journal of biological chemistry 271.46 (1996 Nov 15): 28969-76.

    Immunocytochemical studies of the Na-K-Cl cotransporter of shark kidney.
    Forbush B 3rd
    The American journal of physiology 270.6 Pt 2 (1996 Jun): F927-36.

    Letter: Isolated ventricular inversion with situs solitus.
    Wilkinson JL
    British heart journal 37.11 (1975 Nov): 1202.

    Redistribution of immunofluorescence of CFTR anion channel and NKCC cotransporter in chloride cells during adaptation of the killifish Fundulus heteroclitus to sea water.
    Cozzi RR
    The Journal of experimental biology 205.Pt 9 (2002 May): 1265-73.

    Hypotonic shock mediation by p38 MAPK, JNK, PKC, FAK, OSR1 and SPAK in osmosensing chloride secreting cells of killifish opercular epithelium.
    Hoffmann EK
    The Journal of experimental biology 208.Pt 6 (2005 Mar): 1063-77.

    Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and CFTR anion channel.
    Kaneko T
    The Journal of experimental biology 208.Pt 11 (2005 Jun): 2023-36.

    Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater.
    Kaneko T
    The Journal of experimental biology 208.Pt 20 (2005 Oct): 3851-8.

    Variation in salinity tolerance, gill Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and mitochondria-rich cell distribution in three salmonids Salvelinus namaycush, Salvelinus fontinalis and Salmo salar.
    McCormick SD
    The Journal of experimental biology 210.Pt 6 (2007 Mar): 1015-24.

    Phorbol 12-myristate 13-acetate-induced endocytosis of the Na-K-2Cl cotransporter in MDCK cells is associated with a clathrin-dependent pathway.
    Matthews JB
    American journal of physiology. Cell physiology 298.1 (2010 Jan): C85-97.

    Identification of a sea urchin Na(+)/K(+)/2Cl(-) cotransporter (NKCC): microfilament-dependent surface expression is mediated by hypotonic shock and cyclic AMP.
    Hofman P
    The Journal of experimental biology 204.Pt 1 (2001 Jan): 147-56.

    Exercise modulates chloride homeostasis after spinal cord injury.
    Houlé JD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.27 (2014 Jul 2): 8976-87.

    Differential expression of Na<sup>+</sup>/K<sup>+</sup>/Cl<sup>-</sup> cotransporter 1 in neurons and glial cells within the superficial spinal dorsal horn of rodents.
    Antal M
    Scientific reports 10.1 (2020 Jul 16): 11715.

    Immunohistochemical characterization and change in location of branchial ionocytes after transfer from freshwater to seawater in the euryhaline obscure puffer, Takifugu obscurus.
    Zhao Z
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 190.5 (2020 Sep): 585-596.

    Corticosteroids modulate the secretory processes of the rat intrahepatic biliary epithelium.
    Benedetti A
    Gastroenterology 122.4 (2002 Apr): 1058-69.

    Dynamics of Na(+),K(+),2Cl(-) cotransporter and Na(+),K(+)-ATPase expression in the branchial epithelium of brown trout (Salmo trutta) and Atlantic salmon (Salmo salar).
    Cramb G
    The Journal of experimental zoology 293.2 (2002 Jul 1): 106-18.

    Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis).
    Borski RJ
    Journal of experimental zoology. Part A, Comparative experimental biology 301.12 (2004 Dec 1): 979-91.

    Distinct Na+/K+/2Cl- cotransporter localization in kidneys and gills of two euryhaline species, rainbow trout and killifish.
    Goss GG
    Cell and tissue research 334.2 (2008 Nov): 265-81.

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